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molecular characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

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Biomedical and Environmental Sciences 2014年第4期27卷 231-239页

作者： LI Ming Hua FU Shi Hong CHEN Wei Xin WANG Huan Yu CAO Yu Xi LIANG Guo Dong State Key Laboratory for Infectious Disease Prevention and Control Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease

Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus （JEV） genotype V. Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis. Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% （G I, KV1899） to 79.7% （G III, JaGAr01）, for the nucleotide sequences, and from 90.0%（G I, KV1899） to 91.8%（G III, JaGAr01） for the amino acid sequences. The open reading frame （ORF） of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides （encoded with a serine residue） were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3’-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V. Conclusion The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.

关键词： Japanese encephalitis virus Genotype V molecular characterization

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molecular characterization of polar heteroatom species in oilsands bitumen-derived vacuum residue fractions by Fourier transform ion cyclotron resonance mass spectrometry

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Petroleum Science 2019年第5期16卷 1196-1207页

作者： Fang Zheng Warren Chung Eusebio Palmisano Dahai Dong Quan Shi Zhiming Xu Keng H.Chung State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing 102249China Well Resources Inc EdmontonCanada Cenovus Energy Inc CalgaryCanada Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of SciencesQingdao 266101China

A Canadian in situ oilsands bitumen-derived vacuum residue(VR)was subjected to supercritical fluid extraction and fractionation(SFEF)into 13 extractable fractions and an unextractable end-cut and characterized by positive-and negative-ion electrospray ionization(ESI)Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS).The results of negative-ion ESI FT-ICR MS showed that the N1 class species was the most abundant and the multifunctional group compounds,such as N1 O1,N1 O2,N1 S1,N1 S2,and N2 class species became abundant as the SFEF fraction became *** positive-ion ESI mode,the relative abundance of N1 class species decreased gradually in the heavy SFEF fractions while that of multifunctional group compounds *** relative abundance of N4 V1 O1 increased dramatically in heavy fractions and the *** distributions of polar heteroatom species of VR derived from oilsands bitumen were similar with those of VR derived from the Venezuela Orinoco extra heavy oil.

关键词： molecular characterization Canadian oilsands bitumen SFEF ESI FT-ICR MS

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molecular characterization, tissue and developmental expression profiles of cryptochrome genes in wing dimorphic brown planthoppers, Nilaparvata lugens

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Insect Science 2016年第6期23卷 805-818页

作者： Jing-Jing Xu Gui-Jun Wan Ding-Bang Hu Juan He Fa-Jun Chen Xian-Hui Wang Hong-Xia Hua Wei-Dong Pan Beijing Key Laboratory of Bioelectromagnetics Institute of Electrical Engineering Chinese Academy of Sciences Beijing University of Chinese Academy of Sciences Beijing Department of Entomology College of Plant Protection Nanjing Agricultural University Nanjing Collge of Plant Science & Technology Huazhong Agricultural University Wuhan State College of Plant Protection Northwest Agriculture and Forest University Yangfing Shaanxi State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

Cryptochromes （CRYs） are blue and UV light photoreceptors, known to play key roles in circadian rhythms and in the light-dependent magnetosensitivity of insects. Two novel cryptochrome genes were cloned from the brown planthopper, and were given the designations of Nlcryl and Nlcry2, with the accession numbers KM108578 and KM108579 in GenBank. The complementary DNA sequences ofNlcryl andNlcry2 are 1935 bp and 2463 bp in length, and they contain an open reading frame of 1629 bp and 1872 bp, encoding amino acids of 542 and 623, with a predicted molecular weight of 62.53 kDa and 70.60 kDa, respectively. Well-conserved motifs such as DNA-photolyase and FAD-binding-7 domains were observed in Nlcry1 and Nlcry2. Phylogenetic analysis demonstrated the proteins of Nlcry1 and Nlcry2 to be clustered into the insect＇s cryptochrome 1 and cryptochrome 2, respectively. Quantitative polymerase chain reaction showed that the daily oscillations of messenger RNA （mRNA） expression in the head of the brown planthopper were mild for Nlcryl, and modest for Nlcry2. Throughout all developmental stages, Nlcryl and Nlcry2 exhibited extreme fluctuations and distinctive expression profiles. Cryptochrome mRNA expression peaked immediately after adult emergence and then decreased subsequently. The tissue expression profiles of newly emerged brown planthopper adults showed higher expression levels of CRYs in the head than in the thorax or abdomen, as well as significantly higher levels of CRYs in the heads of the macropterous strain than in the heads of the brachypterous strain. Taken together, the results of our study suggest that the two cryptochrome genes characterized in the brown planthopper might be associated with developmental physiology and migration.

关键词： cryptochrome migration molecular characterization Nilaparvata lugens wing dimorphism

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Origin and molecular characterization of the human-infecting H6N1 infl uenza virus in Taiwan

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Protein & Cell 2013年第11期4卷 846-853页

作者： Weifeng Shi Yi Shi Ying Wu Di Liu George F.Gao School of Medical Sciences Taishan Medical CollegeTaian 271016China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Beijing Institutes of Life Sciences Chinese Academy of SciencesBeijing 100101China Network Information Center Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Offi ce of Director-General Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China

In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in ***,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus *** the present report,we performed phylogenetic and coalescent analy-ses of this virus and compared its molecular profi le/char-acteristics with other closely related *** characterization of H6N1 revealed that it is a typical avian infl uenza virus of low pathogenicity,which might not rep-licate and propagate well in the upper airway in *** analysis revealed that the virus clusters with A/chicken/Taiwan/A2837/2013(H6N1)in seven genes,except *** the PB1 gene,A/Taiwan/2/2013 was clus-tered with a different H6N1 lineage from A/chicken/Taiwan/A2837/*** a previous study demonstrated that the PB2,PA,and M genes of A/Taiwan/2/2013 might be derived from the H5N2 viruses,coalescent analyses revealed that these H5N2 viruses were derived from more recent strains than that of the ancestor of A/Taiwan/2/***,we propose that A/Taiwan/2/2013 is a reassor-tant from different H6N1 lineages circulating in chickens in ***,compared to avian isolates,a sin-gle P186L(H3 numbering)substitution in the hemaggluti-nin H6 of the human isolate might increase the mammali-an receptor binding and,hence,this strain’s pathogenicity in ***,human infection with this virus seems an accidental event and is unlikely to cause an infl uenza ***,its co-circulation and potential reas-sortment with other infl uenza subtypes are still worthy of attention.

关键词： molecular characterization phylogenetic analysis coalescent analysis H6N1 infl uenza virus Taiwan

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Detection and molecular characterization of Cronobacter sakazakiiIsolated from Powdered Infant Formula (PIF) from North Central Region, Nigeria

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Advances in Microbiology 2020年第7期10卷 307-317页

作者： Simon I. Enem Chidozie O. Ogbu Chinwe E. Okoli Ene Godwin Gabriel K. Omeiza Pascal U. Umeakuana Wesley D. Nafarnda Department of Veterinary Public Health and Preventive Medicine University of Abuja Abuja Nigeria Departments of Veterinary Medicine Faculty of Veterinary Medicine University of Abuja Abuja Nigeria

Cronobacter sakazakii is an emerging ubiquitous and opportunistic pathogen that currently contaminates a wide spectrum of foods including powdered milk and poses a lethal threat to neonates, the elderly and persons with immune deficiencies. They cause life threatening neonatal meningitis, septicemia, and necrotizing enterocolitis. A total of 360 samples of powdered infant formula were collected from postnatal hospital attendees reconstituting the PIF for their children in the North Central region of Nigeria where cases of infant mortality are very high and presenting as enterocolitis and diarrhea. Pre-enriched samples were cultured in chromogenic Cronobacter broth and were then further sub-cultured into a chromogenic Cronobacter sakazakii agar. They were positive, exhibiting yellowish cultures typical of Cronobacter sakazakii. Biochemical tests of the isolates were also carried out and indicated the presence of Cronobacter sakazakii. The isolates were then characterized molecularly using specie specific PCR detection of Cronobacter sakazakii. The targeted genes of interest were ompA gene and CPA gene. The isolates tested showed bands for <

关键词： Detection molecular characterization Cronobacter sakazakii Powdered Infant Formula (PIF)

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Preliminary Phenotypic and SNP-Based molecular characterization of Maize (Zea maysL.)-Mexicana (Zea maysSSP. Mexicana) Introgression Lines under Inbred Background of 48-2

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American Journal of Plant Sciences 2021年第7期12卷 1073-1089页

作者： Abdoul Raouf Sayadi Maazou Ju Qiu Ling Ai Mohammad G. Arabzai Mengya Qian Hameed Gul Xuemei Du Fangyu Xing Jiarui Yan Keyi Zhang Ziqi Zhang Qiannan Ma Tianhui Huang Hong Duan Qianlin Xiao Zhizhai Liu College of Agronomy and Biotechnology Southwest University Chongqing China Pan African University Life and Earth Sciences Institute (Including Health and Agriculture) University of Ibadan Ibadan Nigeria International Institute of Tropical Agriculture (IITA) Ibadan Nigeria Paktia University Gardiz Afghanistan

Wild relatives possess potential genetic diversity for maize (Zea mays L.) improvement. characterization of maize-mexicana introgression lines (ILs) is of great value to diversify the genetic base and improve the maiz... 详细信息

Wild relatives possess potential genetic diversity for maize (Zea mays L.) improvement. Characterization of maize-mexicana introgression lines (ILs) is of great value to diversify the genetic base and improve the maize germplasm. Four maize-mexicana IL generations, i.e. BC1, BC2, BC3, and RIL, were constructed under the elite inbred background of 48-2, elite inbred line that is widely used in maize breeding in Southwestern China, and were phenotyped in different years and genotyped with 56110 SNPs. The results indicated that 48-2 had higher phenotypic performances than all the characterized ILs on most of the agronomic traits. Compared with other ILs, BC2 individuals exhibited more similar performance to 48-2 on most traits and possessed the highest kernel ratio (66.5%). Population structure and principal component analysis indicated that BC3 individuals gathered closer to 48-2 and exhibited the lowest mexicana-introgression frequency (0.50%), while BC2 (29.06%) and RIL (18.52%) showed higher introgression frequency. The high level of genetic diversity observed in the maize-mexicana ILs demonstrated that Z. mays ssp. mexicana can serve as a potential source for the enri

关键词： Maize (Zea mays L.) Mexicana (Zea mays SSP. Mexicana) Introgression Lines Phenotypic characterization molecular characterization

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Tissue tropism and molecular characterization of a Japanese encephalitis virus strain isolated from pigs in southwest China

Tissue tropism and molecular characterization of a Japanese ...

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第七届中国畜牧科技论坛

作者： Yuan lei Wu rui Liu hanyang Wen xintian Huang xiaobo yiping Wen Ma xiaoping Yan qigui Huang yong Zhao qin Cao sanjie Research Center of Swine Disease College of Veterinary MedicineSichuan Agricultural University Laboratory of Zoonosis College of Veterinary MedicineSichuan Agricultural University

Since September 2012,an epidemic has been spreading among swine in a pig farm located in Sichuan province,southwest China,which has resulted in abortion,stillbirth,and fetal *** brains of stillborn pigs were collected and a previously unknown Japanese encephalitis virus(JEV),namely SCYA201201,was *** to the results of agarose gel diffusion precipitation,indirect immunofluorescence analysis,neutralization testing,reverse transcription PCR(RT-PCR) amplification,and physical and chemical testing,the virus was conformed to have the characteristics of *** virus titer in BHK-21 cells was 10 PFU/ml and the median lethal dose(LD50) to 3-week-old and 7-dayold mice was 1.99 loglO and 1.02 loglO PFU/LD50,*** results of tissue tropism for mice showed that the viral load in the brain was significantly higher than other organs,indicating that the isolate was strongly ***,the complete genome sequence of the isolate was determined and compared with other JEV *** analysis showed that the isolate belongs to genotype 1 and may be an imported *** isolate had88.4%nucleotide identity with the Chinese vaccine strain ***,there were 69 amino acid substitutions compared with the strain *** substitutions indicated that SCYA201201 was highly neurovirulent and infective,in accordance with the results of animal testing.

关键词： Japanese encephalitis virus isolate tissue tropism molecular characterization

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Distribution and molecular characterization of Citrus yellow vein clearing virus in Yunnan Province of China

Distribution and Molecular Characterization of Citrus yellow...

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中国植物病理学会2019年学术年会

作者： Wang Qin Wang Yingli Yang Zhen He Shaoguo Wu Qiang Li Jifen Li Zhengwen Zhou Yan National Citrus Engineering Research Center Citrus Research InstituteSouthwest University Anyue Lemon Science and Technology Institute Xinping Yi-Dai Autonomous County Agricultural Bureau

In 2009,a new citrus viral disease caused by Citrus yellow vein clearing virus（CYVCV） was first discovered in Yunnan province of *** this study,a survey was conducted in27 orchards from Yunnan province from April 2017 to September *** all,45 of a total of 513 citrus samples were tested positive for CYVCV by ***,the complete genome sequences of 6 CYVCV isolates from different hosts were *** of the whole genome sequences of these6 CYVCV isolates as well as 34 isolates previously reported from around the world revealed the sequence identity ranged from 96.9%-99.8% at nucleotide level,indicating that there is a very low level of sequence heterogeneity among CYVCV isolates of different hosts in Yunnan province.

关键词： Citrus yellow vein clearing virus distribution molecular characterization

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Chromosome location, molecular characterization, polymorphisms and expression of G protein-coupled receptor 54 (GPR54) as a candidate gene for puberty in pigs

Chromosome location, molecular characterization, polymorphis...

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湖北省遗传学会、江西省遗传学会2006年学术年会暨学术讨论会

作者： S.-J. LI, J. REN. L.-S. HUANG Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nangchang, 330045, P. R. China

G-protein coupled receptor (GPR54) is a member of the rhodopsin family and plays a key role in activating the pulsatile release of hypothalamic gonadotropin-releasing hormone, which is crucial for puberty onset. The aim of this study was to characterize GPR54 and provide a starting point for evaluating the association of the variants in this gene with puberty in pigs. GPR54 was assigned to SSC2q21-24 by radiation hybrid mapping, and the most significantly linked marker is SW395 at a

关键词： GPR54 Chromosome location as a candidate gene for puberty in pigs molecular characterization polymorphisms and expression of G protein-coupled receptor 54

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Inheritance and molecular characterization of a novel acetohydroxyacid synthase(AHAS)gene responsible for resistance to AHAS herbicides in rapeseed(Brassica napus L.)

Inheritance and molecular characterization of a novel acetoh...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： HUANG Qian-xin Lyn Jin-yang SUN Yan-yan WANG Hong-mei GUO Yuan QU Gao-ping HU Sheng-wu State Key Laboratory of Crop Stress Biology in Arid Areas College of AgronomyNorthwest A&F University

Utilization of post-emergence herbicides is an effective and economical way to control weeds in rapeseed ***,the availability of selective herbicides for oilseed rape is quite limited due to the shortage of herbicide-resistant cultivars in *** point mutation in acetohydroxyacid synthase(AHAS) gene could be achieved by non-GMO(non-genetically modified organism) way and lead to AHAS-inhibition herbicides-resistance in many *** present study,a tribennuron-methyl(TBM) resistant mutant K5 was screened from ethyl methyl sulfonate mutagenic rapeseed(Brassica napus L.) cultivar Zhongshuang 9(ZS9).The inheritance analysis indicated that TBM resistance was controlled by one dominant nuclear *** sequences analysis revealed that a novel substitution of C with T at position 544 from the translation start site in BnAHAS1 were identified mutant K5,which leading to the alteration of Pro with Ser at position 197 in BnAHAS1(refer to Arabidopsis thaliala sequence).Herbicides-spraying experiments revealed that ZS9 was killed with the treatment of 2.0 mg/L TBM whereas TBM resistance of K5 was almost 100 times than that of control ZS9,and K5 also showed a certain cross-resistance to bensulfuron-methyl and ***1 transgenic Arabidopsis plants exhibited a much higher TBM resistance than its wild-type at 5-6 leaf stage,which confirmed that the single point substitution in BnAHASl was responsible for herbicides-resistance of K5.A CAPS(cleaved amplified polymorphic sequence) marker,which can quickly distinguish both heterozygous and homozygous mutated alleles in BnAHAS1,was developed based on restriction sites of endonuclease Ava ***,a fast way to screen novel TBM-resistant plants in candidate library at their cotyledon stage by treated with 0.5 mg/L TBM in greenhouse was *** general,our research lay a foundation for developing herbicide-resistance rapeseed cultivars with independent intellectual property rights,and confi

关键词： rapeseed(Brassica napus L.) herbicide resistance tribennuron-methyl acetohydroxyacid synthase inheritance molecular characterization

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