Detection and Molecular Characterization of <i>Cronobacter sakazakii</i>Isolated from Powdered Infant Formula (PIF) from North Central Region, Nigeria
Detection and Molecular Characterization of <i>Cronobacter sakazakii</i>Isolated from Powdered Infant Formula (PIF) from North Central Region, Nigeria作者机构：Department of Veterinary Public Health and Preventive Medicine University of Abuja Abuja Nigeria Departments of Veterinary Medicine Faculty of Veterinary Medicine University of Abuja Abuja Nigeria
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2020年第10卷第7期
页 面：307-317页
学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学]
主 题：Detection Molecular Characterization Cronobacter sakazakii Powdered Infant Formula (PIF)
摘 要：Cronobacter sakazakii is an emerging ubiquitous and opportunistic pathogen that currently contaminates a wide spectrum of foods including powdered milk and poses a lethal threat to neonates, the elderly and persons with immune deficiencies. They cause life threatening neonatal meningitis, septicemia, and necrotizing enterocolitis. A total of 360 samples of powdered infant formula were collected from postnatal hospital attendees reconstituting the PIF for their children in the North Central region of Nigeria where cases of infant mortality are very high and presenting as enterocolitis and diarrhea. Pre-enriched samples were cultured in chromogenic Cronobacter broth and were then further sub-cultured into a chromogenic Cronobacter sakazakii agar. They were positive, exhibiting yellowish cultures typical of Cronobacter sakazakii. Biochemical tests of the isolates were also carried out and indicated the presence of Cronobacter sakazakii. The isolates were then characterized molecularly using specie specific PCR detection of Cronobacter sakazakii. The targeted genes of interest were ompA gene and CPA gene. The isolates tested showed bands for
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