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Pressure control strategies in biosafety level-3 large-scale production facilities for animal vaccines

Biosafety and Health 2020年第3期2卷 124-129页

作者： Lei Liang Xin Feng Yi Li China Academy of Building Research Beijing 100013China

This paper summarized the pressure control methods used in 16 large-scale production facilities in China for vaccines against foot-and-mouth disease virus and highly pathogenic avian influenza *** presented the pressure control modes,with their characteristics,of the"constant supply"mode-variable exhaust air volume system and the"constant supply"mode-constant exhaust air volume *** paper analyzed the influence of common problems(e.g.,pressure reference zero drift,positive pressure reversal and failure to restore the original normal working conditions after working condition conversion)on system stability during pressure control in BSL-3 large-scale production facilities and their solutions,providing references for the design and implementation of similar construction projects under the new situation.

关键词： BSL-3 facility large scale production Working condition conversion Negative pressure Animal vaccine

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Study on Propagation of Chicken Infectious Bursal Disease Virus on Vero Cells Using Microcarriers in Fermentor

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Agricultural Sciences in China 2002年第6期1卷 684-689页

作者： SHI Gang, WANG Hong-jun and SUN Hui-ling( Institute of Animal Husbandry and Veterinary Sciences , Beijing Academy of Agricultural and Forestry Sciences ,Beijing 100089 , P. R. China Institute of Radiation Medicine, Academy of MilitaryMedical Sciences, Beijing 100850 , P. R. China) Institute of Animal Husbandry and Veterinary Sciences Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 P. R. China Institute of Radiation Medicine Academy of Military Medical Sciences Beijing 100850 P. R. China Institute of Animal Husbandry and Veterinary Sciences Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 P. R. China

It was in flask optimization tests proved that 2% serum, pH 7.0, 5:10 000 inoculation concentration of infectious bursal disease virus (IBDV) and 108 hours cultivation for IBDV harvest after its inoculation were the optimal conditions when IBDV was propagated on Vero cells. 250 ml self-made spinner bottle and 5 L stirring fermentor tests proved that IBDV could maintain higher liters for a long time and the highest liters of IBDV in a spinner bottle and a fermentor were 8.875 and 8.58 ( - lgTCID50/0.1 ml) respectively when IBDV was proliferated on Vero cells using 2 g/L microcarriers in a spinner bottle and a fermentor and was cultivated under the optimum conditions obtained from flask tests after Vero cells had developed a confluent monolayer on microcarriers, which were at least one titer higher than the highest titer in the traditional rolling bottle. All these results suggested that this technology could be applied to large scale production for IBDV.

关键词： Infectious bursal disease virus (IBDV) Fermentor Vero cell Titer large scale production

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large-scale Synthesis of Efficient CO2 Reduction Membrane Catalyst for Industrial Applications

Large-scale Synthesis of Efficient CO2 Reduction Membrane Ca...

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中国化学会·第一届全国二氧化碳资源化利用学术会议

作者： Changming Zhao Yuen Wu Department of Chemistry iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) University of Science and Technology of China

Single atom catalysts show great promise for achieving high efficiency and selectivity in the CO RR process. Herein, we found for the first time that single Ni sites exhibited excellent activity and selectivity for the CO RR. We successfully synthesize a hierarchical, self-supported, flexible, and programmable catalyst through a solid-state diffusion strategy. This catalyst can be directly used as a binder-free electrode and exhibits superb catalytic CO electroreduction performance. Directly synthesized by bulk metal foil, this catalyst is scalable to meet the industrial demand. In our work, Ni SAs/N-C has been successfully synthesized by ionic exchange of Zn nodes and adsorbed Ni salts. Compared with Ni NPs and Ni foam, Ni SAs are capable of selectively reducing CO with excellent current density and FE. Then, we prepared a hierarchical, self-supported, flexible, and programmable H-CPs catalyst containing abundant single Ni sites by a solid-state diffusion between the loose N-doped carbon phase and a compact bulk Ni metal. This approach shed light to the construction of efficient and stable catalyst directly from the non-precious bulk materials and show great promise for industrial-scale production.

关键词： CO2 electroreduction Ni single atom large scale production

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Extraction of Polysaccharides from Spirulina with Boiling Water

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Agricultural Science & Technology 2014年第6期15卷 1043-1045页

作者：唐玉邦虞利俊裴勤徐磊王恒义江苏省农业科学院农业设施与装备研究所江苏南京210014

[Objective] The aim of this study was to develop a simple and effective method for the polysaccharide extraction from Spirulina. [Method] The polysaccha- rides were directly extracted from the fresh algae mat or dry powder of Spirulina in boiling water. The contents and quality of the extracted polysaccharides were measured. [Result] On average, 236.06 g of polysaccharide was extracted from 25 kg of fresh Spirulina mat, with a yield rate of 0.94%, while 191.95 g of polysac- charide was extracted from 2.5 kg of dry powder of Spirulina with a yield rate of 0.77%. The polysaccharide content in the extract of fresh Spirulina mat was 12.56% （according to glucose content）, while that of the dry powder was 12.38% （according to glucose content）; the glucose was produced during the hydrolysis of Spirulina polysaccharide. [Conclusion] Extraction polysaccharide from Spirulina with boiling wa- ter greatly reduces the use of ethanol, and the possibility of pollution from the ex- ogenous non-food chemical reagents, so that the extracted polysaccharides can be used as food materials. This method makes it possible to establish the production line for Spirulina polysaccharide.

关键词： Spirulina polysaccharide large scale production Extraction by boilingwater Identification

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应用新兴生物技术推进林木种苗生产现代化——杂交鹅掌楸体细胞胚胎发生大规模生产优质种苗的成功例子

应用新兴生物技术推进林木种苗生产现代化——杂交鹅掌楸体细胞胚...

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首届国际生物经济高层论坛

作者：施季森陈金慧陈志国家林业局和江苏省林木遗传和基因工程重点实验室南京林业大学

杂交鹅掌楸是南京林业大学在20世纪六十年代创造的属内种间杂交种,由于在生长,材质,树型,抗性等方面存在十分明显的杂种优势,受到业界的广泛瞩目,尤其是在中国南部已成为园林景观树种和林业工业界用材树种的首选树种之一。

关键词： Liriodendron Hybrids Somatic embryogenesis Cytobiology Ultra structure Plantlet regeneration large scale production Bioreactor

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