Study on Propagation of Chicken Infectious Bursal Disease Virus on Vero Cells Using Microcarriers in Fermentor

作     者：SHI Gang, WANG Hong-jun and SUN Hui-ling( Institute of Animal Husbandry and Veterinary Sciences , Beijing Academy of Agricultural and Forestry Sciences ,Beijing 100089 , P. R. China Institute of Radiation Medicine, Academy of MilitaryMedical Sciences, Beijing 100850 , P. R. China) 

作者机构：Institute of Animal Husbandry and Veterinary Sciences Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 P. R. China Institute of Radiation Medicine Academy of Military Medical Sciences Beijing 100850 P. R. China Institute of Animal Husbandry and Veterinary Sciences Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 P. R. China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2002年第1卷第6期

页      面：684-689页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

主　　题：Infectious bursal disease virus (IBDV) Fermentor Vero cell Titer Large scale production 

摘      要：It was in flask optimization tests proved that 2% serum, pH 7.0, 5:10 000 inoculation concentration of infectious bursal disease virus (IBDV) and 108 hours cultivation for IBDV harvest after its inoculation were the optimal conditions when IBDV was propagated on Vero cells. 250 ml self-made spinner bottle and 5 L stirring fermentor tests proved that IBDV could maintain higher liters for a long time and the highest liters of IBDV in a spinner bottle and a fermentor were 8.875 and 8.58 ( - lgTCID50/0.1 ml) respectively when IBDV was proliferated on Vero cells using 2 g/L microcarriers in a spinner bottle and a fermentor and was cultivated under the optimum conditions obtained from flask tests after Vero cells had developed a confluent monolayer on microcarriers, which were at least one titer higher than the highest titer in the traditional rolling bottle. All these results suggested that this technology could be applied to large scale production for IBDV.