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Detection of k-ras gene point mutation in fine needle aspiration and pancreatic juice by sequence special primer method and its clinical significance

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World Journal of Gastroenterology 2000年第6期6卷 917-919页

作者： Xun Liang Liu Cun Cai Dai Yi Miao Jing Hui Du Zhao Song Zhang Shu Zhen Chen Department of General Surgery the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210024Jiangsu ProvinceChina The Molecular Biology Research Center of Nanjing Medical University Nanjing 210029Jiangsu ProvinceChina

INTRODUCTIONThe point mutation rate of k-ras gene at codon 12 inpancreatic adenocarcinoma is reported to be as highas 90%,and with no mutations in normalpancreas tissues or other pancreatic disorders.Wehave detected t... 详细信息

关键词： pancreatic neoplasms/diagnosis polymerase chain reaction biopsy needle genes ras pancreatic diseases pancreatic juice gene amplification cytodiagnosis

Effects of tomato juice on the pharmacokinetics of CYP3A4-substrate drugs

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Asian Journal of Pharmaceutical Sciences 2017年第5期12卷 464-469页

作者： Atsuko Ohkubo Tomomi Chida Hidetomo Kikuchi Tadashi Tsuda Katsuyoshi Sunaga Laboratory of Pharmacotherapy Department of Clinical Dietetics and Human Nutrition Faculty of Pharmaceutical Sciences Josai University

We previously demonstrated that tomato juice(TJ) contains potent mechanism-based inhibitor(s) of CYP3A4. In this study, we investigated the effects of TJ and grapefruit juice(GFJ)on the pharmacokinetics of the CYP3A4-substrate drugs, nifedipine(NFP) and midazolam(MDZ), in male Wistar rats. Oral administration of GFJ 90 min before the intraduodenal administration of NFP or MDZ increased the area under the concentration–time curves(AUCs)of NFP and MDZ by 32.4% and 89.4%, respectively. TJ increased MDZ blood concentrations and AUC after intraduodenal MDZ administration;however, it had no effect on NFP. When MDZ and NFP were intravenously administered, GFJ significantly increased the AUC of MDZ,but only slightly increased that of NFP. In contrast, TJ only slightly increased the AUC of MDZ.These results suggest that, similar to GFJ, TJ influences the pharmacokinetics of CYP3A4-substrate drugs;however, it may be a drug-dependent partial effect.

关键词： Food–drug interactions Tomato juice Grapefruit juice Nifedipine Midazolam Pharmacokinetic interactions

A novel aeration strategy in repeated-batch fermentation for efficient ethanol production from sweet sorghum juice

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Chinese Journal of Chemical Engineering 2019年第7期27卷 1651-1658页

作者： Niphaphat Phukoetphim Naulchan Khongsay Pattana Laopaiboon Lakkana Laopaiboon Department of Biotechnology Faculty of TechnologyKhon Kaen UniversityKhon Kaen 40002Thailand Graduate School Khon Kaen UniversityKhon Kaen 40002Thailand Fermentation Research Center for Value Added Agricultural Products Khon Kaen UniversityKhon Kaen 40002Thailand

To improve the efficiency of ethanol production in a batch fermentation from sweet sorghum juice under a very high gravity(VHG)condition(~290 g/L of total sugar)by Saccharomyces cerevisiae NP01,repeatedbatch fermentation under an aerated condition(2.5 vvm for the first 4 h during every cycle)was done in a5-L fermenter.The average ethanol concentration(P),productivity(Qp)and yield(Yp/s)for five successive cycles were 112.31 g/L,1.55 g/L·h^-1 and 0.44,respectively with 80.97%sugar consumption.To complete sugar consumption,the total sugar of the juice was reduced to a high gravity(HG)level(~240 g/L).The results showed that yeast extract was not necessary for ethanol production,and aeration during every other cycle i.e.,alternating cycles,was sufficient to promote both yeast growth and ethanol production.The average P,Qpand Yp/svalues for eight successive cycles with aeration during alternating cycles were97.58 g/L,1.98 g/Láh and 0.41,respectively with 91.21%sugar consumption.The total fatty acids in the yeast cells under the aerated condition were^50%higher than without aeration,irrespective the initial sugar concentration,whereas the ergosterol contents under aeration condition were^29%to 49%higher than those without aeration.

关键词： Aeration Nitrogen supplement Repeated-batch fermentation Saccharomyces cerevisiae Sweet sorghum juice

Impact of continuous local lavage on pancreatic juice-related postoperative complications: Three case reports

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World Journal of Clinical Cases 2019年第17期7卷 2526-2535页

作者： Tomohide Hori Kohei Ogawa Hidekazu Yamamoto Hideki Harada Kazuyoshi Matsumura Michihiro Yamamoto Masahiro Yamada Takefumi Yazawa Katsutoshi Kuriyama Masaki Tani Daiki Yasukawa Yasuyuki Kamada Yuki Aisu Ryotaro Tani Ryuhei Aoyama Shinnosuke Nakayama Yudai Sasaki Koki Nishimoto Masazumi Zaima Department of Surgery Shiga General HospitalMoriyama 524-8524Japan Department of Hepatobiliary Pancreatic and Breast Surgery Ehime UniversityToon 791-0295Japan Department of Gastroenterology and Hepatology Shiga General HospitalMoriyama 524- 8524Japan Department of Surgery Shiga University of Medical ScienceOtsu 520-2192Japan Department of Surgery Kyoto University Graduate School of MedicineKyoto 606- 8507Japan

BACKGROUND Postoperative pancreatic leakage readily results in intractable pancreatic fistula and subsequent intraperitoneal abscess.This refractory complication can be fatal;therefore,intensive treatment is important.Continuous local lavage (CLL) has recently been reevaluated as effective treatment for severe infected pancreatitis,and we report three patients with postoperative intractable pancreatic fistula successfully treated by CLL.We also discuss our institutional protocol for CLL for postoperative pancreatic fistula.CASE SUMMARY The first patient underwent subtotal stomach-preserving pancreaticoduodenectomy,and pancreatic leakage was observed postoperatively.Intractable pancreatic fistula led to intraperitoneal abscess,and CLL near the pancreaticojejunostomy site was instituted from postoperative day (POD) 8.The abscess resolved after 7 d of CLL.The second patient underwent distal pancreatectomy.Pancreatic leakage was observed,and intractable pancreatic fistula led to intraperitoneal abscess near the pancreatic stump.CLL was instituted from POD 9,and the abscess resolved after 4 d of CLL.The third patient underwent aneurysmectomy and splenectomy with wide exposure of the pancreatic parenchyma.Endoscopic retrograde pancreatic drainage was performed on POD 15 to treat pancreatic fistula;however,intraperitoneal abscess was detected on POD 59.We performed CLL endoscopically via the transgastric route because the percutaneous approach was difficult.CLL was instituted from POD 63,and the abscess resolved after 1 wk of CLL.CONCLUSION CLL has therapeutic potential for postoperative pancreatic fistula.

关键词： Surgery Pancreas Pancreatic fistula Pancreatic juice Postoperative complications Case report

Potential prognostic biomarkers in pancreatic juice of resectable pancreatic ductal adenocarcinoma

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World Journal of Clinical Oncology 2017年第3期8卷 255-260页

作者： Shefali Agrawal Hepatobiliary and Pancreatic Surgery Department of Surgical OncologyIndraprastha Apollo Hospitals

Despite potentially curative surgery pancreatic cancer has a dismal prognosis.Serum cancer antigen 19-9(CA 19-9) correlates with tumor burden,resectability and survival in patients with pancreatic ductal adenocarcinoma.Identification of novel biomarkers may facilitate early diagnosis of pancreatic cancer and improve survival.Pancreatic juice is a rich source of cancer-specific proteins rendering it a promising tool for identifying biomarkers.Recent proteomic and micro RNA expression analyses have identified several biomarkers of potential diagnostic and prognostic value.Tumor markers CA 19-9 and carcinoembryonic antigen(CEA) are widely used in the characterization of premalignant and malignant lesions of the pancreas.Elevated level of CEA in bile is a marker for malignancy and a predictor of hepatic recurrence.The potential value of CA 19-9,CEA and lactate dehydrogenase as prognostic biomarkers in pancreatic juice and bile is unknown.Specimens of pancreatic juice and bile can be readily collected during surgical resection of the tumor.Profiling of pancreatic juice and bile to identify novel prognostic biomarkers may improve selection of patients for adjuvant therapy with a favorable impact on overall survival in patients diagnosed with pancreatic cancer.

关键词： Prognostic biomarkers Pancreatic juice Bile Pancreatic adenocarcinoma Surgery

Chemical composition, antioxidant activity and GC-MS analysis of juice and peel oil of grapefruit varieties cultivated in India

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Journal of Integrative Agriculture 2019年第7期18卷 1634-1642页

作者： Shahnawaz Ahmed H S Rattanpal Khalid Gul Rouf Ahmad Dar Akash Sharma Department of Fruit Science Punjab Agricultural UniversityLudhiana 141004India Department of Processing and Food Engineering Punjab Agricultural UniversityLudhiana 141004India Food Process Engineering Laboratory School of Applied Life SciencesGyeongsang National UniversityJinju 660701Republic of Korea Department of Microbiology Punjab Agricultural UniversityLudhiana 141004India Department of Fruit Science Sher-e-Kashmir University of Agricultural Sciences and TechnologyJammu 180009India

Citrus family especially Grapefruit,has attained considerable attention due to the presence of a number of essential components that have cardiovascular and anti-hypertensive properties.The juice and essential oil extracted from eight recently released grapefruit cultivars were used to study physicochemical and antioxidant properties.The total soluble solids(TSS),titratable acidity(TA) and pH of juice samples extracted from various grapefruit varieties differed significantly.The refractive index,specific gravity and optical rotation values for the oil varied from 1.473 to 1.396,0.863 to 0.847 and +93 to +86,respectively.The percent 2,2-diphenyl-1-picrylhydrazyl radical activity(% DPPH activity) and ferric reducing antioxidant power(FRAP) values for grapefruit juice and peel oil varied from 24.06 to 18.79,2.91 to 1.44 mmol g^–1 and 84.87 to 74.73,7.76 to 5.73 mmol g^-1,respectively.There were significant differences in physicochemical,antioxidant properties and volatile profiles of extracted juice and oil.The oil exhibited higher DPPH and FRAP values than the juice.Among different components identified which accounted for over 99% of the volatile fraction,limonene,myrcene,and benzopyran were major components in all oil samples.

关键词： grapefruit juice essential oil physicochemical properties antioxidant properties GC-MS analysis

Exposure to gastric juice may not cause adenocarcinogenesis of the esophagus

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World Journal of Gastroenterology 2013年第15期19卷 2419-2424页

作者： Peng Cheng Jian-Sheng Li Lian-Feng Zhang Yong-Zhong Chen Jun Gong Department of Gastroenterology the First Hospital of Zhengzhou University Department of Gastroenterology the Second Hospital of Xi'an Jiao Tong University

AIM: To determine the effects of gastric juice on the development of esophageal adenocarcinoma (EAC). METHODS: A animal model of duodenogastroesophageal reflux was established in Sprague-Dawley rats undergoing esophagoduodenostomy. The development of EAC and forestomach adenocarcinoma was investigated 40 wk after the treatment. Intraluminal pH and bile of the forestomach were measured. RESULTS: There were no significant differences in pH (t=0.117, P=0.925) or bile (χ2=0.036, P=0.85) in the forestomach before and 40 wk after esophagoduodenostomy. There were also no significant differences between the model and controls during esophagoduodenostomy or 40 wk after esophagoduodenostomy. The incidence of intestinal metaplasia (88%) and intestinal metaplasia with dysplasia and adenocarcinoma (28%) in the esophagus in the model was higher than in the controls 40 wk after surgery (χ2=43.06, P juice exposure may not cause intestinal metaplasia with dysplasia or adenocarcinoma of the forestomach and may not be related to EAC.

关键词： Intestinal metaplasia Gastric juice Pathogenesis Esophageal adenocarcinoma Gastroesophageal reflux

juice, Ethanol, and Grain Yield Potential of Five Sweet Sorghum (<i>Sorghum bicolor</i>[L.] Moench) Cultivars

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Journal of Sustainable Bioenergy Systems 2013年第2期3卷 113-118页

作者： Laban K. Rutto Yixiang Xu Michael Brandt Shuxin Ren Maru K. Kering Agriculture Research Station Virginia State University Petersburg USA

Sweet sorghum (Sorghum bicolor [L.] Moench) accumulates fermentable sugars in the stem and is increasingly being studied as a potential source of feedstock for bioethanol production. The objective of this study was to evaluate biomass and grain yield in five sweet sorghum cultivars (Dale, M81E, Sugar Drip, Della and Keller) and to determine quality of extractable juice and grain. Randomized complete block experiments were performed in the summer of 2009, 2010, and 2011. Leaf dry weight varied with year and cultivar and averaged 6177 kg·ha-1. Fresh stem weight ranged from 21 to 54 Mg·ha-1 with a mean across years and cultivars of 32.9 Mg·ha-1. Variations in stem weight were correlated with extractable juice volumes that ranged from 10 to 24 m3·ha-1. juice Brix values fell within a narrow range (14% - 19%) across years and cultivars with an average of 15.6%. In all production years, theoretical sugar and ethanol yield were always numerically higher for Keller and M81E. Grain yield was lowest in Keller (90 kg·ha-1), but ranged from 400 to 1300 kg·ha-1 in other cultivars with a mean of 584 kg·ha-1 across years. However, Keller had the highest starch content with a lower proportion of resistant starch in the grain. Except for Keller, the cultivars tested are potential sources of both fermentable sugars and grain.

关键词： Sweet Sorghum Cultivar Biomass juice and Grain Yield Ethanol Yield Potential

Optimization of Nutrients in Fermentative Lactic Acid Production Using Oil Palm Trunk juice as Substrate

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Advances in Bioscience and Biotechnology 2014年第12期5卷 957-965页

作者： Nisa Saelee Klanarong Sriroth Department of Biotechnology Faculty of Agro-Industry Kasetsart University Bangkok Thailand Department of Biotechnology Institute of Agricultural Technology Walailak University Nakhon Si Thammarat Thailand

Complex nutrients e.g. carbon, nitrogen, peptides, vitamin and salts significantly play important roles in lactic acid production efficiency depending on types of microorganism and sources of raw material. In this study oil palm trunk juice and Lactobacillus rhamnosus TISTR 108 (ATTC 10863) were utilized for lactic acid production. Additional nutrients including peptone, yeast extract and mixed salts were tested. Response surface methodology involving Box Behkhen Design (BBD) was applied to examine the optimal condition. Prediction of optimization was performed using full quadratic regression equation. The predicted maximum lactic acid concentration was obtained at 64.05 g·l-1 within a period of 48 h under an optimal condition of 10 g·l-1 peptone with mixed salts containing 0.4 g·l-1 MgSO4·7H2O, 0.1 g·l-1 MnSO4·4H2O, 3 g·l-1 K2HPO4, 3 g·l-1 KH2PO4 and 3 g·l-1 CH3COONa·3H2O in 250 ml shake flask using CaCO3 as a titrant. Verification of optimization condition was performed in 2 l fermenter using Ca(OH)2 as neutralizing agent. Increase in lactic acid fermentation was achieved at 92.81 g·l-1 at 48 h cultivation. The lactic acid yield and volumetric productivity were 0.94 g·g-1 and 1.91 g l-1·h-1, respectively. This suggests that OPT juice is potentially used as carbon and nutrient sources for lactic acid production.

关键词： Lactic Acid Oil Palm Oil Palm Trunk juice OPT Lactobacillus rhamnosus Box Behkhen Design BBD