Detection of k-ras gene point mutation in fine needle aspiration and pancreatic juice by sequence special primer method and its clinical significance

作     者：Xun Liang Liu Cun Cai Dai Yi Miao Jing Hui Du Zhao Song Zhang Shu Zhen Chen 

作者机构：Department of General Surgerythe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210024Jiangsu ProvinceChina The Molecular Biology Research Center of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2000年第6卷第6期

页      面：917-919页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Natural Science Foundation of Jiangsu Scientific Committee 

主　　题：pancreatic neoplasms/diagnosis polymerase chain reaction biopsy needle genes ras pancreatic diseases pancreatic juice gene amplification cytodiagnosis 

摘      要：INTRODUCTIONThe point mutation rate of k-ras gene at codon 12 inpancreatic adenocarcinoma is reported to be as highas 90%,and with no mutations in normalpancreas tissues or other pancreatic *** detected the presence of k-ras gene