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Mesenchymal-epithelial transition factor amplification correlates with adverse pathological features and poor clinical outcome in colorectal cancer

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WORLD JOURNAL OF GASTROINTESTINAL SURGERY 2024年第5期16卷 1395-1406页

作者： Yu, Qiu-Xiao Fu, Ping-Ying Zhang, Chi Li, Li Huang, Wen-Ting Canc Hosp Chinese Acad Med Sci & Peking Union Med Coll Natl Canc Ctr Natl Clin Res Ctr CancDept Pathol 113 Baohe Ave Shenzhen 518116 Guangdong Peoples R China Shenzhen Hosp Chinese Acad Med Sci & Peking Union Med Coll 113 Baohe Ave Shenzhen 518116 Guangdong Peoples R China

BACKGROUND Colorectal cancer (CRC) is the third most common cancer and the second most common cause of cancer-related mortality worldwide. Mesenchymal-epithelial transition factor (MET) gene participates in multiple tumor biology and shows clinical potential for pharmacological manipulation in tumor treatment. MET amplification has been reported in CRC, but data are very limited. Investigating pathological values of MET in CRC may provide new therapeutic and genetic screening options in future clinical practice. AIM To determine the pathological significance of MET amplification in CRC and to propose a feasible screening strategy. METHODS A number of 205 newly diagnosed CRC patients undergoing surgical resection without any preoperative therapy at Shenzhen Cancer Hospital of Chinese Academy of Medical Sciences were recruited. All patients were without RAS/RAF mutation or microsatellite instability-high. MET amplification and c-MET protein expression were analyzed using fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), respectively. Correlations between MET aberration and pathological features were detected using the chi-squared test. Progression free survival (PFS) during the two-year follow-up was detected using the Kaplan-Meier method and log rank test. The results of MET FISH and IHC were compared using one-way ANOVA. RESULTS Polysomy-induced MET amplification was observed in 14.4% of cases, and focal MET amplification was not detected. Polysomy-induced MET amplification was associated with a higher frequency of lymph node metastasis (LNM) (P < 0.001) and higher tumor budding grade (P = 0.02). In the survival analysis, significant difference was detected between patients with amplified- and non-amplified MET in a two-year follow-up after the first diagnosis (P = 0.001). C-MET scores of 0, 1+, 2+, and 3+ were observed in 1.4%, 24.9%, 54.7%, and 19.0% of tumors, respectively. C-MET overexpression correlated with higher frequency of LNM (P = 0.002), but no significa

关键词： Colorectal cancer MET amplification Pathological features Prognosis Fluorescence in situ hybridization CELL LUNG-CANCER C-MET ADENOCARCINOMA MUTATIONS PROGNOSIS INVASION CRITERIA

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Diagnosis of the accurate genotype of HKαα carriers in patients with thalassemia using multiplex ligation-dependent probe amplification combined with nested polymerase chain reaction

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Chinese Medical Journal 2020年第10期133卷 1175-1181页

作者： Dong-Mei Chen Shi Ma Xiang-Lan Tang Ji-Yun Yang Zheng-Lin Yang Clinical Medical School Southwest Medical UniversityLuzhouSichuan 646000China The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical SciencesChengduSichuan 610072China Natural Products Research Center Institute of Chengdu BiologySichuan Translational Medicine HospitalChinese Academy of SciencesChengduSichuan 610072China.

Background:Patients carrying the HongKongαα(HKαα)allele and-α3.7/αααanti-4.2 could be misdiagnosed as-α3.7/ααby the current conventional thalassemia detection methods,leading to inaccurate genetic counseling and an incorrect prenatal diagnosis.This study was aimed to accurately analyze the genotypes of HKααcarriers and-α3.7/αααanti-4.2.Methods::Samples were collected in our hospital from July 2017 to October 2019.Twenty-four common types of Chinese thalassemia were screened by gap-polymerase chain reaction(Gap-PCR)and reverse dot blot(RDB).Anti-4.2 multiplex-PCR was used to confirm carriers of theαααanti-4.2 duplication with-α3.7 deletion.Two-round nested PCR and multiplex ligation-dependent probe amplification(MLPA)were applied to accurately identify and confirm their genotypes.For data analysis,we used descriptive statistics and Fisher’s exact tests.Results::Two thousand five hundred and forty-four cases were identified as thalassemia in 5488 peripheral blood samples.The results showed thatα,β,andαβcompound thalassemia were identified in 1190(46.78%),1286(50.55%),and 68(2.67%)cases,respectively.A total of 227 samples from thalassemia patients were identified as-α3.7/ααby Gap-PCR,and the genotypes of two samples were uncertain.There was a difference between Gap-PCR and combined groups(Gap-PCR combined with nested PCR and MLPA)in detecting HKαα(P<0.05).Among the 229 patients,20 patients were identified as HKααcarriers and one was identified as-α3.7/ααα anti-4.2 by two-round nested PCR and MLPA,including 15 patients with HKαα/αα,three with HKαα/αα and β-thalassemia coinheritance,one with HKαα/-SEA,one with HKαα/-α4.2 andβ-thalassemia coinheritance,and one with-α3.7/αααanti-4.2 and β-thalassemia coinheritance.Conclusions::αααanti-4.2 and HKααgenotypes of patients carrying-α3.7 need to be detected to reduce the misdiagnosis rate of patients carrying HKααand-α3.7/αααanti-4.2 alleles.More accurate genetic counseling can be provided in the clinic using nested PCR combined with MLPA.

关键词： Thalassemia HongKongαα Nested polymerase chain reaction Multiplex ligation-dependent probe amplification Gene dosage

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激光、啁啾脉冲放大、超快光学和诺贝尔奖

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科学通报 2019年第14期64卷 1433-1440页

作者：赵昆中国科学院物理研究所北京凝聚态物理国家研究中心北京100190

2018年诺贝尔物理学奖表彰了两项在激光物理领域的突破性发明,其中啁啾脉冲放大是获得超强超短激光脉冲的核心技术.本文介绍了自1960年世界上第一台激光器问世以来,人们在追求超短激光脉冲过程中一系列关键成果,及其在现代科学研究中的... 详细信息

2018年诺贝尔物理学奖表彰了两项在激光物理领域的突破性发明,其中啁啾脉冲放大是获得超强超短激光脉冲的核心技术.本文介绍了自1960年世界上第一台激光器问世以来,人们在追求超短激光脉冲过程中一系列关键成果,及其在现代科学研究中的重要应用.

关键词： laser chirped pulse amplification ultrafast optics ultrafast dynamics

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Rapid identification of Dendrobium officinale using Loop-Mediated Isothermal amplification (LAMP) method

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Chinese Journal of Natural Medicines 2019年第5期17卷 337-345页

作者： YANG Lu WU Wen-Ru ZHOU Hua LAI Hui-Li FU Fei School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine Faculty of Chinese Medicine Macao University of Science and Technology State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and Technology School of Nursing Guangdong Food and Drug Vocational College

Dendrobium officinale is not only an ornamental plant, but also a valuable medicinal herb that is widely used in traditional Chinese medicine. However, distinguishing D. officinale from other Dendrobium species is usually a difficult task. In this study,we developed a rapid identification protocol for D. officinale using the loop-mediated isothermal amplification(LAMP) method. A set of primers were specifically designed to detect a modified internal transcribed spacer region of D. officinale at 65 ℃ within 40 min after adding SYBR~? Green I, which was used for the detection of D. officinale. Unlike commonly used adulterants, reaction mixtures containing D. officinale DNA changed from orange to green, and this color change was easily observed with the naked eye. Thus, this methodology can be used to accurately differentiate D. officinale from other Dendrobium species, is quick as all D. officinale samples were amplified within 40 min, and specific as samples of the adulterants were not amplified. The specificity of this LAMP-based method was confirmed by testing 17 samples of D. officinale and 32 adulterant samples from other Dendrobium species. This LAMP-based rapid identification method does not require expensive equipment or specialized techniques and can be used in field surveys for accurate and fast on-site identification.

关键词： Dendrobium officinale ITS Loop-mediated isothermal amplification Identification

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amplification of in-plane seismic ground motion by group cavities in layered half-space (Ⅰ)

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Earthquake Science 2012年第4期25卷 275-285页

作者： Jianwen Liang Ji Zhang Zhenning Ba Department of Civil Engineering Tianjin University Tianjin Key Laboratory of Civil Engineering Structures and New Materials

amplification of in-plane seismic ground motion by underground group cavities in layered half-space is studied both in frequency domain and time domain by using indirect boundary element method （IBEM）, and the effect of cavity interval and spectrum of incident waves on the amplification are studied by numerical examples. It is shown that there may be large interaction between cavities, and group cavities with certain intervals may have significant amplification to seismic ground motion. The amplification of PGA （peak ground acceleration） and its PRS （peak response spectrum） can be increased up to 45.2% and 84.4%, for an example site in Tianjin, under the excitation of Taft wave and E1 Centro wave; and group cavities may also affect the spectra of the seismic ground motion. It is suggested that the effect of underground group cavities on design seismic ground motion should be considered.

关键词： underground group cavity amplification in-plane seismic ground motion time domain frequency domain indirect boundary element method （IBEM）

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Dynamic amplification factors for a system with multiple-degrees-of-freedom

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Earthquake Engineering and Engineering Vibration 2020年第2期19卷 363-375页

作者： Zhang Chao Hao Hong Bi Kaiming Yan Xueyuan College of Civil Engineering Fuzhou UniversityFuzhou 350108China Centre for Infrastructure Monitoring and Protection School of Civil and Mechanical EngineeringCurtin UniversityKent StreetBentley WA 6102Australia Key Laboratory of Urban Security and Disaster Engineering of MOE Beijing University of TechnologyBeijing 100124China

It is well-known that the responses of a structure are different when subjected to a static load or a sudden step load.The dynamic amplification factor(DAF),which is defined as the ratio of the amplitude of the vibratory response to the static response,is normally used to depict the dynamic effect.For a single-degree-of-freedom system(SDOF)subjected to a sudden dynamic load,the maximum value of DAF is 2.Many design guidelines therefore use 2 as an upper bound to consider the dynamic effect.For a civil engineering structure,which is normally a multiple-degrees-of-freedom(MDOF)system,the DAF may exceed 2 in certain circumstances.The adoption of 2 as the upper bond as suggested by the design guidelines therefore may lead to unsafe structural design.Very limited studies systematically investigate the DAF of a MDOF sysCorrespondence to:Bi Kaiming,Centre for Infrastructure Monitoring and Protection,School of Civil and Mechanical Engineering,Curtin University,Kent Street,Bentley WA 6102,Australia Tel:(+61)892665139 E-mail:kaiming.bi@curtin.***.autem.This study theoretically investigates the DAF of a MDOF system when it is subjected to a step load based on the fundamental theory of structural dynamics.The condition on which the DAF may exceed 2 is defined.Two numerical examples and one experimental study of a cable-stayed bridge subjected to sudden cable loss are presented to illustrate the problem.

关键词： dynamic amplification factors(DAF) multiple-degrees-of-freedom(MDOF) step load

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amplification of plasmid DNA bound on soil colloidal particles and clay minerals by the polymerase chain reaction

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Journal of Environmental Sciences 2007年第11期19卷 1326-1329页

作者： CAI Peng HUANG Qiao-yun LU Yan-du CHEN Wen-li JIANG Dai-hua LIANG Wei State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070 China Key Laboratory of Subtropical Agricultural Resources a~ld Environment Huazhong Agricultural University Wuhan 430070 China

Polymerase chain reaction （PCR） was used to amplify a 600-base pair （bp） sequence of plasmid pGEX-2T DNA bound on soil colloidal particles from Brown soil （Alfisol） and Red soil （Ultisol）, and three different minerals （goethite, kaolinite, montmorillonite）. DNA bound on soil colloids, kaolinite, and montmorillonite was not amplified when the complexes were used directly but amplification occurred when the soil colloid or kaolinite-DNA complex was diluted, 10- and 20-fold. The montmorillonite-DNA complex required at least 100-fold dilution before amplification could be detected. DNA bound on goethite was amplified irrespective of whether the complex was used directly, or diluted 10- and 20-fold. The amplification of mineral-bound plasmid DNA by PCR is, therefore, markedly influenced by the type and concentration of minerals used. This information is of fundamental importance to soil molecular microbial ecology with particular reference to monitoring the fate of genetically engineered microorganisms and their recombinant DNA in soil environments.

关键词： adsorption amplification mineral PCR plasmid DNA soil colloid

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amplification effect on SBS and Rayleigh scattering in the backward pumped distributed fiber Raman amplifier

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Chinese Optics Letters 2009年第5期7卷 393-395页

作者：张在宣龚华平 College of Optical and Electronic Technology China Jiliang University

The amplification effect on stimulated Brillouin scattering （SBS） and Rayleigh scattering in the backward pumped G652 fiber Raman amplifier （FRA） is studied. The pump source is a 1427.2-nm fiber Raman laser whose power is tunable between 0 - 1200 mW, and the signal source is a tunable narrow spectral bandwidth （〈 10 MHz） external cavity laser （ECL）. The Rayleigh scattering lines are amplified by the FRA and Stokes SBS lines are amplified by the FRA and the fiber Brillouin amplifier. The total gain of SBS lines is the production of the gain of Raman amplifier and that of Brillouin amplifier. In experiment, the SBS gain is about 42 dB and the saturation gain of 25-km G652 backward FRA is about 25 dB, so the gain of fiber Brillouin amplifier is about 17 dB.

关键词： amplification Fiber lasers Fibers Piezoelectric actuators Pumping (laser) Raman spectroscopy Rayleigh scattering Scattering

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Clinical significance of MET gene amplification in metastatic or locally advanced gastric cancer treated with first-line fluoropyrimidine and platinum combination chemotherapy

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Chinese Journal of Cancer Research 2019年第4期31卷 620-631页

作者： Seyoung Seo Min-Hee Ryu Baek-Yeol Ryoo Yangsoon Park Young Soo Park Young-Soon Na Chae-Won Lee Ju-Kyung Lee Yoon-Koo Kang Departments of Oncology Asan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505Republic of Korea Department of Pathology Asan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505Republic of Korea Asan Institute for Life Science Asan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505Republic of Korea

Objective: To investigate the clinical significance of MET gene amplification in patients with gastric cancer in the palliative setting.Methods: MET amplification was assessed using fluorescence in situ hybridization(FISH) in 50 patients and quantitative polymerase chain reaction(qPCR) in 326 patients;259 patients treated with first-line fluoropyrimidine and platinum were included for survival analysis.Results: The results of FISH and qPCR indicated that the c-MET/CEP7 ratio was correlated with gene copy number. The optimal cutoff value for the copy number using qPCR to detect MET gene amplification with FISH was 5(κ=0.778, Pamplification with a copy number of >5 detected by qPCR. MET-amplified gastric cancer was associated with an Eastern Cooperative Oncology Group(ECOG) performance status(PS) score of ≥2(33.3% vs. 10.5% P=0.007), peritoneal metastasis(76.2% vs. 46.2%, P=0.008), and elevated bilirubin levels(28.6% vs. 7.3%, P=0.006). The median overall survival(OS) and progression-free survival(PFS) were 11.9 and 5.6 months, respectively. MET-amplified gastric cancer was not associated with survival outcomes [hazard ratio(HR)=0.68, 95% confidence interval(95% CI): 0.35-1.32,P=0.254 for PFS;HR=0.68, 95% CI: 0.35-1.32, P=0.251 for OS].Conclusions: qPCR can be used to detect MET gene amplification. MET amplification was not a predictor of poor prognosis in patients with metastatic or unresectable gastric cancer.

关键词： MET amplification advanced gastric cancer prognosis quantitative real-time polymerase chain reaction

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Noiseless linear amplification for the single-photon entanglement of arbitrary polarization–time-bin qudit

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Chinese Physics B 2019年第1期28卷 210-217页

作者：陈灵泉盛宇波周澜 School of Science Nanjing University of Posts and TelecommunicationsNanjing 210003China College of Electronic and Optical Engineering&College of Microelectronics Nanjing University of Posts and TelecommunicationsNanjing 210003China Key Laboratory of Broadband Wireless Communication and Sensor Network Technology(Ministry of Education) Nanjing University of Posts and TelecommunicationsNanjing 210003China Institute of Signal Processing Transmission Nanjing University of Posts and TelecommunicationsNanjing 210003China

Single-photon entanglement(SPE) is an important source in quantum communication. In this paper, we put forward a single-photon-assisted noiseless linear amplification protocol to protect the SPE of an arbitrary polarization–time-bin qudit from the photon transmission loss caused by the practical channel noise. After the amplification, the fidelity of the SPE can be effectively increased. Meanwhile, the encoded polarization–time-bin features of the qudit can be well preserved. The protocol can be realized under the current experimental conditions. Moreover, the amplification protocol can be extended to resist complete photon loss and partial photon loss during the photon transmission. After the amplification, we can not only increase the fidelity of the target state, but also solve the decoherence problem simultaneously. Based on the above features, our amplification protocol may be useful in future quantum communication.

关键词： single-photon entanglement noiseless linear amplification polarization–time-bin qudit complete and partial photon loss

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