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Detection of Staphylococcus aureus in dairy products by Polymerase Chain Reaction Assay

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Agricultural Sciences in China 2007年第7期6卷 857-862页

作者： YANG Yang SU Xu-dong YUAN Yao-wu KANG Chun-yu LI Ying-jun ZHANG wei ZHONG Xiao-ying Laboratory for Microorganism Detection College of Food Science and Technology Agricultural University of Hebei Baoding 071001 P. R. China Baoding Center for Disease Control and Prevention in Hebei Province Baoding 071001 P.R.China

A polymerase chain reaction （PCR） assay was employed for direct detection of Staphylococcus aureus without enrichment in dairy products. A solvent extraction procedure was successfully modified for the extraction of Staphylococcus aureus DNA from artificially contaminated whole milk, skim milk, and cheese. A primer targeting the thermostable nuclease gene （nuc） was used in the PCR. A DNA fragment of 279 bp was amplified. The PCR product was confirmed by DNA sequencing. In this study, the PCR, GB-4789.10-94, Perifilm *** Plate, and Baird-Parker ＋ RPF Agar were compared. The sensitivity of the PCR was 10 CFU mL^-1 of whole milk, skim milk, and 55 CFU g^-1 of cheese. The developed methodology allowed for detection of Staphylococcus aureus in dairy products in less than 6 h. The time taken for the development of this PCR assay was 12-24 h, less than the time taken by the general PCR assay using the enrichment method, and the coincidence rate of this developed PCR was 94.3%, the sensitivity was 100%. It was a rapid, sensitive, and effective method for PCR to detect Staphylococcus aureus in milk and milk products.

关键词： PCR detection dairy products Staphylococcus aureus

Search of Salmonella in Meat and dairy products

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Journal of Life Sciences 2013年第1期7卷 76-83页

作者： Sonia Khosrof Sbei Rim Wejden Suihi Department of Food and Technology Tunis High School of Health Sciences and Technology Rabta 1007 Tunisia Department of Hygiene and Environmental Protection in Tunis Tunisia. (Researchers in Salmonella)

The epidemiology of Salmonella involves food, especially meat and dairy products. These have a high salmonella outbreak in the summer. The majority of Salmonella enteritis in young children occurs in the form of scattered cases. At least 25% of summer gastroenteritis in young children is caused by Salmonella. In Western Europe, S. typhimurium accounts for nearly 70% of isolates. The objective of this study was to investigate the Salmonella contaminating meat and dairy products as well as define the percentages of contamination by Salmonella from 2004 to 2006, by the Department of Hygiene of Tunis. For this study, we collected samples in various motherboard solutions, and conducted a pre-nonselective enrichment in selective enrichment in isolation and identification and ultimately biochemical confirmation. One hundred and sixty two samples, 125 samples of meat products including 68 samples of red meat （beef and sheep, beef, offal and Merguez） and 57 samples of poultry （chicken and turkey）, 37 samples of milk products which included 32 samples of cheese and five samples milk, were analysed microbiologically from 2004 to 2006, in the Health Service in Tunis. These analyses include the detection and enumeration of Salmonella. From 2004 to 2006, the rate of infections by the Salmonella, meat and dairy products were： 55.9% for red meat, 71.9% for poultry, 68.8% for cheeses, 40% for milk. The meat of poultry is contaminated with Salmonella that the cheeses, which are more than red meats, which are also more contaminated than milk. This might be due to a lack of hygiene throughout the circuit processing （slaughter, transport, processing, etc.）. The increased risk of contamination of milk products by Salmonella is proportional to the manipulation done on these products. The latter must be handled by pasteurization and sterilization.

关键词： Salmonella meat products dairy products contamination.

Study on Quality and Safety Trust Early Warning of dairy products Based on Statistical Process Control

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Asian Agricultural Research 2017年第10期9卷 63-67,71页

作者： Xiao FAN College of Economics and Management Chongqing University of Arts and Sciences

In order to solve such problems as lack of dynamic evaluation system in evaluation of quality and safety trust of dairy products,and weak awareness of prevention,it is necessary to introduce the statistical process control into the quality and safety trust evaluation system of dairy products,and establish quality and safety trust early warning model for dairy products,so as to determine the control limit of control chart and carry out early warning according to eight criteria. According to the empirical results,the statistical process control is helpful for finding the hidden process risks and providing the necessary basis for enterprises taking positive measures to raise the confidence of consumers.

关键词： Statistical process control dairy products Quality and safety trust Early warning

Research Progress on Detection Methods of Melamine and Cyanuric Acid Residue in dairy products and Feedstuff

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Animal Husbandry and Feed Science 2013年第2期5卷 76-78页

作者： WANG Hua-dong FENG Xiao-chun Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences College of Technology and Engineering Lanzhou University of Technology

[Objective] More accurate, rapid and sensitive method of melamine and cyanuricacid residue in dairy products and feedstuff were re- viewed. [ Method] Physicochemical properties, metabolism, uses, harm and detection methods of melamine and cyanuric acid were analyzed and described. [ Result] Melamine and cyanuric acid, when used alone, were slightly toxic, but long -term intake could lead to animal reproductive and urinary system damage. [ Condusion] Establishing a more sensitive, fast and easy to popularize detection method for elarnine and cyanuricacid res- idue in dairy products and feedstuff was necessary.

关键词： Melamine Cyanuric acid dairy products Feedstuff Detection method

The Consumption Preferences of Milk and dairy products of Undergraduate Students

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Journal of Food Science and Engineering 2012年第1期2卷 10-14页

作者： Mustafa Mortas Fehmi YaZlCl Department of Food Engineering Ondokuz Mayls University Samsun 55139 Turkey

Human has different stages in their life as from babyhood to adult. The healthy and balanced diet preferences of people play an important role in each human life parts. Especially milk and dairy products are necessary for human health because of their nutrition value and body functions. One of the parts of people life is university life. Starting university is a turning point in terms of eating habits, since food choices responsibility of university students increases in this period. According to the researches, increasing availability of fast foods, changes in living arrangements, life experiences, expectations, preferences and beliefs related with food selections are most common reasons regarding dietary choices in this young adults group. University students had drinking milk habits in primary school but most of them lose their habits during university life. The students＇ first preferences were cheese and then yogurt and finally milk. A very little part of university students consume milk and dairy products regularly. The consumption habits and milk and dairy product catering of university students were examined. The consumption preferences of milk and dairy products of the students in different Universities are reviewed in this review.

关键词： Milk dairy products consumption preferences undergraduate students.

Calcium-fortified fresh milk ameliorates postmenopausal osteoporosis via regulation of bone metabolism and gut microbiota in ovariectomized rats

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Food Science and Human Wellness 2024年第3期13卷 1258-1270页

作者： Qishan Wang Bin Liu Xianping Li Junying Zhao Zongshen Zhang Weicang Qiao Xinyue Wei Lijun Chen School of Bioengineering Dalian Polytechnic UniversityDalian 116034China National Engineering Research Center of dairy Health for Maternal and Child Beijing Sanyuan Foods Co.Ltd.Beijing 100163China Beijing Engineering Research Center of dairy Beijing Sanyuan Foods Co.Ltd.Beijing 100163China

The aging of the global population has made postmenopausal osteoporosis prevention essential;however,pharmacological treatments are ***,we evaluate the effect of calcium-fortified fresh milk(FM)in ameliorating postmenopausal osteoporosis in a rat model established using bilateral *** 3 months of FM(containing vitamin D,and casein phosphopeptides,1000 mg Ca/100 g)or control milk(110 mg Ca/100 g milk)supplementation,bone changes were assessed using dual-energy X-ray absorptiometry,microcomputed tomography,and bone biomechanical *** results revealed that FM can regulate bone metabolism and gut microbiota composition,which act on bone metabolism through pathways associated with steroid hormone biosynthesis,relaxin signaling,serotonergic synapse,and unsaturated fatty acid ***,FM administration significantly increased bone mineral content and density in the lumbar spine and femur,as well as femoral compressive strength,while improving femoral trabecular bone parameters and ***,we found that the effects may be due to increased levels of estrogen,bone formation marker osteocalcin,and procollagen typeⅠN-propeptide,and decreased expression of the bone resorption marker C-telopiptide and tartrate-resistant acid phosphatase ***,the findings suggest that FM is a potential alternative therapeutic option for ameliorating postmenopausal osteoporosis.

关键词： dairy products Calcium Vitamin D Bone turnover markers Gut microbiota Postmenopausal osteoporosis

A Survey on Occurrence of Melamine and Its Analogues in Tainted Infant Formula in China

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Biomedical and Environmental Sciences 2009年第2期22卷 95-99页

作者： MELAMINE ANALYSIS GROUP Institute of Nutrition and Food Safety Chinese Center for Disease Control and Prevention

Objective To investigate the occurrence and concentrations of melamine and its analogues in tainted infant formula and to identify the etiologic factors for the urinary stones epidemic in infants and young children in China in 2008. Methods Sanlu infant formula samples were collected from families of the affected children in Gansu province, and markets in Gansu and Hebei provinces and Beijing city. Melamine and its analogues, including cyanuric acid, ammeline, and ammelide were measured by gas chromatography/tandem mass spectrometry. Results High prevalence and concentrations of melamine were found in Sanlu infant formula samples, with low concentrations of cyanuric acid, ammeline and ammelide. Melamine were detected in 87 out of 111 Sanlu infant formula samples with a range of 118 to 4 700 mg/kg, Conclusion The results provide strong evidence for melamine as the etiological factor for the urinary stones epidemic in infants and young children in China in 2008.

关键词： Melamine Cyanuric acid dairy products Powdered infant formula China

Novel avocado oil-functionalized yogurt with anti-obesity potential:Technological and nutraceutical perspectives

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Food Bioscience 2022年第6期50卷 161-170页

作者： Manuela Machado Sérgio Sousa Pilar Morais Arménio Miranda Luís M.Rodriguez-Alcalá Ana Maria Gomes Manuela Pintado Universidade Católica Portuguesa CBQF Centro de Biotecnologia e Química Fina-Laboratório AssociadoEscola Superior de BiotecnologiaRua Diogo Botelho 13274169-005PortoPortugal Frulact S.ARua do Outeiro589Gemunde4475-150MaiaPortugal

Studies on bioactive lipids have been increasing in the last years due to their proven health *** purpose of this research work was to develop a yogurt with bioactive lipids with anti-obesity *** this purpose,yogurts were produced with avocado oil in free form or encapsulated as a *** presence of avocado oil enhanced the nutritional quality of yogurts through a reduction(more than 75%)of their atherogenic and thrombogenic *** high nutritional value was particularly evident in samples with bigel,as these yogurts presented eight-fold higher levels of total fatty acids than the yogurts with avocado oil in free *** addition,yogurts with avocado bigel presented better stability throughout the gastrointestinal tract-results showed a higher recovery percentage of oleic acid(47.2%)when compared to 24%in the yogurts with avocado in free *** results from the in vitro studies demonstrated that these functional yogurts can increase the lipolytic rate in adipocytes,translated by an increase of ca.20%in glycerol release in yogurts with ***,the hepatic lipid accumulation in cells subjected to steatosis induced by chloroquine was reduced by 35%in cells treated with yogurt digested *** results showed the potential of these functional yogurts to modulate obesity-related metabolism.

关键词： Lipid metabolism Obesity dairy products Functional foods

近代上海公共租界食品安全管理及成效探析(1870—1937)

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