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Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair

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Bioactive Materials 2023年第2期20卷 208-220页

作者： Qi Wu Xiaoling Fu Xian Li Jing Li Weiju Han Yingjun Wang School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou511442PR China National Engineering Research Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006PR China Key Laboratory of Biomedical Engineering of Guangdong Province South China University of TechnologyGuangzhou510006PR China Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of TechnologyGuangzhou510006PR China

The process of bone repair is highly regulated by a large number of bioactive ***,a“cocktail”of bioactive factors supplemented to the defect sites is desirable for bone *** this regard,small extracellular vesicles(sEVs)derived from mesenchymal stem cells hold great potential in tissue ***,the poor homing and retention of sEVs greatly limited their possible clinical *** the present work,DMPE-PEG-CREKA was inserted into the membrane of sEVs released from adipose-derived mesenchymal stem cells to obtain CREKA functionalized sEVs(CREKA-sEVs),which could target fibrin to accumulate and retain in bone *** results showed that CREKA-sEVs,like sEVs,promoted the osteogenic differentiation of BMSCs,the angiogenic property of HUVECs,and modulated the polarization of macrophages in ***,due to the improved fibrin-binding and retention capacity of CREKA-sEVs,they enhanced the bone repair substantially in the rat femoral defect *** study provided a new strategy to improve the therapeutic efficiency of sEVs and showed that CREKA-sEVs had great application value in bone tissue repair.

关键词： MSCs-derived sEVs Fibrin-targeting CREKA bone repair

Long non-coding RNA HCAR promotes endochondral bone repair by upregulating VEGF and MMP13 in hypertrophic chondrocyte through sponging miR-15b-5p

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Genes & Diseases 2022年第2期9卷 456-465页

作者： Yun Bai Xiaoshan Gong Rui Dong Zhen Cao Ce Dou Chuan Liu Jianmei Li Fei Kang Jingjin Dai Chunrong Zhao Zhansone Tian Jiulin Tan Qijie Dai Shiwu Dong Department of Biomedical Materials Science School of Biomedical EngineeringThird Military Medical UniversityChongqing 400038PR China Department of Orthopedics Southwest HospitalThird Military Medical UniversityChongqing 400038PR China State Key Laboratory of Trauma Burns and Combined InjuryThird Military Medical UniversityChongqing 400038PR China Institute of Trauma Orthopedics Army General Hospital of PLABeijing 100700PR China

Endochondral bone formation is an important route for bone *** emerging evidence has revealed the functions of long non-coding RNAs(lncRNAs)in bone and cartilage development,the effect of lncRNAs in endochondral bone repair is still largely ***,we identified a lncRNA,named Hypertrophic Chondrocyte Angiogenesis-related lncRNA(HCAR),and proved it to promote the endochondral bone repair by upregulating the expression of matrix metallopeptidase 13(Mmp13)and vascular endothelial growth factorα(Vegfa)in hypertrophic ***-HCAR knockdown in hypertrophic chondrocytes restrained the cartilage matrix remodeling and decrease the CD31hiEmcnhi vessels number in a bone repair ***,we proved that lnc-HCAR was mainly enriched in the cytoplasm using fluorescence in situ hybridization(FISH)assay,and it acted as a molecular sponge for ***,in hypertrophic chondrocytes,lnc-HCAR competitively bound to miR-15b-5p to increase Vegfa and Mmp13 *** results proved that lncRNA is deeply involved in endochondral bone repair,which will provide a new theoretical basis for future strategies for promoting fracture healing.

关键词： bone marrow mesenchymal stem cells bone repair Chondrocyte Enchondral bone repair Long non coding RNA

Forsterite-hydroxyapatite composite scaffolds with photothermal antibacterial activity for bone repair

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Journal of Advanced Ceramics 2021年第5期10卷 1095-1106页

作者： Weiye LIU Rongtai ZUO Tanglong ZHU Min ZHU Shichang ZHAO Yufang ZHU School of Materials Science and Engineerings University of Shanghai for Science and Technology Shanghai 200093China State Key L aboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Department of Orthopedics Shanghai Jiao Tong University affiliated Sixth People s HospitalShanghai Jiao Tong UniversityShanghai 200233China

bone engineering scaffolds with antibacterial activity satisfy the repair of bacterial infected bone defects,which is an expected issue in *** this work,3D-printed polymer-derived forsterite scaffolds were proposed to be deposited with hydroxyapatite(HA)coating via a hydrothermal treatment,achieving the functions of photothermal-induced antibacterial ability and *** results showed that polymer-derived forsterite scaffolds possessed the photothermal antibacterial ability to inhibit Staphylococcus aureus(***)and Escherichia coli(***)in vitro,owing to the photothermal effect of free carbon embedded in the *** morphology of HA coating on forsterite scaffolds could be controlled through changing the hydrothermal temperature and the pH value of the reaction solution during hydrothermal ***,HA coating did not influence the mechanical strength and photothermal effect of the scaffolds,but facilitated the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells(rBMSCs)on ***,the HA-deposited forsterite scaffolds would be greatly promising for repairing bacterial infected bone defects.

关键词： forsterite scaffolds hydroxyapatite(HA)coating bone repair photothermal effect antibacterial activity

Phosphate Glass Fibre Composites for bone repair

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Journal of Bionic Engineering 2009年第4期6卷 318-323页

作者： Andrew J.Parsons Ifty Ahmed Papia Haque Ben Fitzpatrick Muhammad I.K.Niazi Gavin S.Walker Chris D.Rudd Division of Materials Mechanics & StructuresFaculty of EngineeringUniversity of NottinghamNottingham NG7 2RDUK

We investigate high-modulus degradable materials intended to replace metals in biomedical *** are typically composites comprising a polylactide(PLA)matrix reinforced with phosphate glass fibres,which provide reinforcement similar to E-glass but are entirely degradable in water to produce,principally,calcium *** have made composites using a variety of fibre architectures,from non-woven random mats to unidirectional fibre *** properties in the region of 30 GPa modulus and 350 MPa strength have been achieved-directly comparable to quoted values for human cortical *** collaboration with other groups we have begun to consider the development of foamed systems with structures mimicking cancellous bone and this has shown significant *** fibres in these foamed structures provide improved creep resistance and reinforcement of the pore *** date the materials have exhibited excellent cellular responses in vitro and further studies are due to include consideration of the surface character of the materials and the influence of this on cell interaction, both with the composites and the glass fibres themselves,which show promise as a standalone porous scaffold.

关键词： biocomposites phosphate glass PLA porous bone repair

Novel Mesoporous Hydroxyapatite/Chitosan Composite for bone repair

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Journal of Bionic Engineering 2012年第2期9卷 243-251页

作者： Jingzhe Zhang Guangyao Liu Qiong Wu Jianlin Zuo Yanguo Qin Jincheng Wang Orthopedic Department China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China

The objective of the present investigation was to evaluate the osteogenic properties of mesoporous Hydroxyapatite/Chitosan （HAJCS） composite in vitro and in vivo. HA/CS composite was successfully prepared and synthesized using a freeze-drying method, and then characterized by Scanning Electron Microscope （SEM）. Results show that the mesoporous HA/CS composite presents high surface area and porosity. The effects of mesoporous HA/CS on early adhesion, proliferation and differentiation of osteoblast cells in vitro were measured. MTT cytotoxicity test and cell adhesion test show that the composite has good biocompatibility and promotes cell viability and proliferation. In vitro tests show that osteoblast-like cells on the composite surfaces are able to adhere, proliferate, and migrate through the pores. These cells maintained similar expression levels of osteoblastic-associated markers namely Collagen type I （COL-I）, bone Morphogenetic Protein 2（BMP-2）. Histologic analysis and radiological analysis in vivo also prove that mesoporous HA/CS composite can be used to repair bone defect as a new kind of bone grafting materials.

关键词： hydroxyapatite/chitosan composite bone repair biocompatibility freeze-drying

Hydrothermal growth of whitlockite coating on β-tricalcium phosphate surfaces for enhancing bone repair potential

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Journal of Materials Science & Technology 2018年第6期34卷 1054-1059页

作者： Xiaoheng Guo Xiao Liu Huichang Gao Xuetao Shi Naru Zhao Yingjun Wang School of Materials Science and Engineering South China University of Technology Guangzhou 510641 China Notional Engineering Research Centre for Tissue Restoration and Reconstruction Guangzhou 510006 China Guangdong Province Key Laboratory of Biomedical Engineering South China University of Technology Guangzhou 510006 China school of Medicine South China University of Technology Guangzhou 510006 China

In this study, we developed a simple approach for the controllable growth of whitlockite （WH） on a β- tricalcium phosphate surface and investigated its cell viability via CCK-8, its live-dead staining and its alkaline phosphatase activity. Herein, WH with controllable morphologies was prepared by regulating the hydrothermal reaction conditions. The results of scanning electron microscopy, X-ray diffraction and X- ray photoelectron spectroscopy indicated that pure hexagonal plates of WH were prepared successfully. In vitro cell experiments showed that WH possessed excellent biocompatibility and effectively promoted the adhesion and proliferation of mouse bone mesenchymal stem cells. The osteogenesis of the WH was also enhanced. The obtained WH was expected to be utilized for promising applications as implantable block materials for bone repair.

关键词： Surface Whitlockite Hydrothermal reaction bone repair

Magnetic responsive scaffolds and magnetic fields in bone repair and regeneration

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Frontiers of Materials Science 2014年第1期8卷 20-31页

作者： Hai-Yan XU Ning GU Department of Biomedical Engineering Institute of Basic Medical Sciences Chinese Academy of Medical Science & Peking Union Medical College Beijing 100005 China State Key Laboratory of Bioelectronics and Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science & Medical Engineering Southeast University Nanjing 210096 China

Increasing evidence shows that magnetic fields and magnetic responsive scaffolds can play unique roles in promoting bone repair and regeneration. This article addresses the synergistic effects of magnetic scaffolds in response to external magnetic fields on the bone regeneration in situ. Additionally, the exploration of using magnetic scaffolds as tools in the bone implant fixation, local drug delivery and mimicking microenvironment of stem cell differentiation are introduced. We also discussed possible underlying mechanisms and perspectives of magnetic responsive scaffolds in the bone repair and regeneration.

关键词： bone repair magnetic scaffold magnetic field physical stimulation

Doxycycline induces bone repair and changes in Wnt signalling

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International Journal of Oral Science 2017年第3期9卷 158-166页

作者： kátia do nascimento gomes ana paula negreiros nunes alves paula góes pinheiro dutra glauce socorro de barros viana faculty of dentistry pharmacy and nursingfederal university of cearáFortalezaBrazil faculty of medicine federal university of cearáFortaleza Brazil

Doxycycline （DOX） exhibits anti-inflammatory and MMP inhibitory properties. The objectives of this study were to evaluate the effects of DOX on alveolar bone repair. Controls （CTL） and DOX-treated （10 and 25 mg. kg- 1） molars were extracted, and rats were killed 7 or 14 days later. The maxillae were processed and subjected to histological and immunohistochemical assays. Hematoxylin-eosin staining （7th day） revealed inflammation in the CTL group that was partly reversed after DOX treatment. On the 14th day, the CTL group exhibited bone neoformation, conjunctive tissue, re-epithelization and the absence of inflammatory infiltrate. DOX-treated groups exhibited complete re-epithelization, tissue remodelling and almost no inflammation. Picrosirius red staining in the DOXlO group （7th and 14th days） revealed an increased percentage of type I and III collagen fibres compared with the CTL and DOX25 groups. The DOXlO and DOX25 groups exhibited increases in osteoblasts on the 7th and 14th days. However, there were fewer osteoclasts in the DOXlO and DOX25 groups on the 7th and 14th days. Wnt-lOb- immunopositive cells increased by 130% and 150% on the 7th and 14th days, respectively, in DOX-treated groups compared with the CTL group. On the 7th day, Dickkopf （Dkk）-I immunostaining was decreased by 63% and 46% in the DOXlO and DOX25 groups, respectively. On the 14th day, 69% and 42% decreases in immunopositive cells were observed in the DOXlO and DOX25 groups, respectively, compared with the CTL group. By increasing osteoblasts, decreasing osteoclasts, activating Wnt lOb and neutralising Dkk, DOX is a potential candidate for bone repair in periodontal diseases.

关键词： alveolar bone loss bone diseases bone repair doxycycline Dickkopf-1 immunohistochemistry Wnt-lOb

Biodegradable porous Zn-1Mg-3βTCP scaffold for bone defect repair:In vitro and in vivo evaluation

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Journal of Materials Science & Technology 2023年第31期162卷 189-202页

作者： Runhua Yao Hui Wang Ruifeng Shan Lin Liu Yuyu Zhao Yonghua Sun Xiaohong Yao Di Huang Ruiqiang Hang Shanxi Key Laboratory of Biomedical Metal Materials College of Materials Science and EngineeringTaiyuan University of TechnologyTaiyuan 030024China Shanxi Xinhua Chemical Defense Equipment Research Institute Co. LtdTaiyuan 030008China Research Center for Nano-Biomaterials&Regenerative Medicine Department of Biomedical EngineeringCollege of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan 030024China Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering Taiyuan 030060China

Zn-based materials are promising as bone repair materials,but their poor mechanical property and bioactivity as well as low degradation rate render the potential *** structural and material design can address the *** this study,porous Zn-1 wt.%Mg-3 vol.%β-TCP scaffolds with 40%and 60%preset porosities were fabricated via heating-press sintering using NaCl particles as space holders,and their mechanical properties,in vitro degradation behavior,cytotoxicity and in vivo osteogenic activities were *** results showed that the actual porosities of the scaffolds were 22%and 50%.Mg exists in the form of Zn 2 Mg and Zn 11 Mg 2,whileβ-TCP evenly distributed in the *** compressive yield strength of scaffolds ranges from approximately 58.46 to 71.04 MPa,which is close to that of cancellous *** in vitro degradation tests showed that the corrosion rate of the scaffolds was in the range of about 2.73-4.28 mm y^(-1).Moreover,the scaffolds not only provided great space for osteoblasts adhesion and proliferation in vitro but also possessed favorable degradability and osteogenic activity in *** porous Zn-1 wt.%Mg-3 vol.%β-TCP scaffolds manifest reliable mechanical properties,desirable degradability,and osteogenic activity,which are promising as next-generation bone repair materials.

关键词： Biodegradation Zn-based composites Porous scaffolds bone repair