Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair

作     者：Qi Wu Xiaoling Fu Xian Li Jing Li Weiju Han Yingjun Wang 

作者机构：School of Biomedical Sciences and EngineeringSouth China University of TechnologyGuangzhou International CampusGuangzhou511442PR China National Engineering Research Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhou510006PR China Key Laboratory of Biomedical Engineering of Guangdong ProvinceSouth China University of TechnologyGuangzhou510006PR China Key Laboratory of Biomedical Materials and Engineering of the Ministry of EducationSouth China University of TechnologyGuangzhou510006PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第20卷第2期

页      面：208-220页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2021YFB3800900) National Natural Science Foundation of China(31971266,51232002) the Key Research and Development Program of Guangzhou(202007020002) Guangdong Province Basic and Applied Research Foundation(2022A1515011925) 

主　　题：MSCs-derived sEVs Fibrin-targeting CREKA Bone repair 

摘      要：The process of bone repair is highly regulated by a large number of bioactive ***,a“cocktailof bioactive factors supplemented to the defect sites is desirable for bone *** this regard,small extracellular vesicles(sEVs)derived from mesenchymal stem cells hold great potential in tissue ***,the poor homing and retention of sEVs greatly limited their possible clinical *** the present work,DMPE-PEG-CREKA was inserted into the membrane of sEVs released from adipose-derived mesenchymal stem cells to obtain CREKA functionalized sEVs(CREKA-sEVs),which could target fibrin to accumulate and retain in bone *** results showed that CREKA-sEVs,like sEVs,promoted the osteogenic differentiation of BMSCs,the angiogenic property of HUVECs,and modulated the polarization of macrophages in ***,due to the improved fibrin-binding and retention capacity of CREKA-sEVs,they enhanced the bone repair substantially in the rat femoral defect *** study provided a new strategy to improve the therapeutic efficiency of sEVs and showed that CREKA-sEVs had great application value in bone tissue repair.