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Methionine as a methyl donor regulates caste differentiation in the European honey bee(apis mellifera)

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Insect Science 2021年第3期28卷 746-756页

作者： Wen-Feng Chen Ying Wang Wei-Xing Zhang Zhen-Guo Liu Bao-Hua Xu Hong-Fang Wang College of Animal Science and Technology Shandong Agricultural UniversityTaianShandong ProvinceChina

Nutrition contributes to honey bee caste differentiation,but the role of individual nutrients is still *** essential amino acid contents,except that of methionine(Met),are greater in royal jelly than worker ***〜3.5 i the Met content in the latter was slightly greater than in the *** is the major raw material used in the synthesis of S-adenosyl-L-methionine,an active methyl donor for DNA methylation,which is an epigenetic driver of caste ***,we tested whether Met regulates caste differentiation in honey bees by determining its effects on the caste development of bees receiving four diets:the basic,basic+0.2%Met,basic+0.2%Met+20 mg/kg 5-azacytidine,and basic+20 mg/kg *** presence of Met decreased the adult bee body length and the numbers of ovarioles,indicating that Met may direct the development of female larvae toward worker *** upregulated expression of SAMS,Dnmtl,and Dnmt3 caused by Met exposure in 4-d-old larvae indicated that the worker-inductive effects of Met may occur through the promotion of DNA *** investigated the co-effects of Met and glucose on bee development,and found that the effects of an increased glucose level on the number of ovarioles and body length did not strengthen the worker-inductive effects caused by *** results contribute to caste development theory and suggest that Met-as a methyl donor一plays a regulatory,but not decisive,role in caste differentiation.

关键词： apis mellifera body length caste differentiation methionine methyl donor ovarioles

Distribution and variability of deformed wing virus of honeybees （apis mellifera） in the Middle East and North Africa

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Insect Science 2017年第1期24卷 103-113页

作者： Nizar Jamal Haddad Adjlane Noureddine Banan Al-Shagour Wahida Loucif-Ayad Mogbel A. A. El-Niweiri Eman Anaswah Wafaa Abu Hammour Dany El-Obeid Albaba Imad Mohamed A. Shebl Abdulhusien Sehen Almaleky Abdullah Nasher Nagara Walid Mohamed Fouad Bergigui Orlando Yanez Joachim R. de Miranda Bee Research Department National Center for Agriculture Research and Extension Baq'a Jordan Department of Biology M'hamed Bougare University of Boumerdes ENS Kouba Algeries Laboratory of Applied Animal Biology University Badji-Mokhtar Annaba Algeria Department of Bee Research Environment Natural Resources and Desertification Research Institute National Centre for Research Khartoum Sudan Faculty of Agriculture and Veterinary Sciences Lebanese University Beirut Lebanon West Bank State of Palestine HalhuI-Hebron District Palestine Department of Plant Protection Suez Canal University Ismailia Egypt Extension Department Qadysia Govemate Agricultural Directorate Iraq Department of Plant Protection Sana'a University Sane'a Yemen National Federation of Tunisian beekeepers Tunis Tunisia Ruchers El Bakri Hay Assalam-Sidi Slimane Rabat Morocco Institute of Bee Health Vetsuisse Faculty University of Bern Bern Switzerland Department of Ecology Swedish University of Agricultural Sciences Uppsala Sweden

Three hundred and eleven honeybee samples from 12 countries in the Middle East and North Africa （MENA）（Jordan, Lebanon, Syria, Iraq, Egypt, Libya, Tunisia, Algeria, Morocco, Yemen, Palestine, and Sudan） were analyzed for the presence of deformed wing virus （DWV）. The prevalence of DWV throughout the MENA region was pervasive, but variable. The highest prevalence was found in Lebanon and Syria, with prevalence dropping in Palestine, Jordan, and Egypt before increasing slightly moving westwards to Algeria and Morocco Phylogenetic analysis of a 194 nucleotide section of the DWV Lp gene did not identify any significant phylogenetic resolution among the samples, although the sequences did show consistent regional clustering, including an interesting geographic gradient from Morocco through North Africa to Jordan and Syria. The sequences revealed several clear variability hotspots in the deduced amino acid sequence, which furthermore showed some patterns of regional identity. Furthermore, the sequence variants from the Middle East and North Africa appear more numerous and diverse than those from Europe.

关键词： apis mellifera deformed wing virus Middle East North Africa prevalence variability

The integrative analysis of microRNA and mRNA expression in apis mellifera following maze-based visual pattern learning

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Insect Science 2014年第5期21卷 619-636页

作者： Qiu-Hong Qin Zi-Long Wang Liu-Qing Tian Hai-Yan Gan Shao-Wu Zhang Zhi-Jiang Zeng Honeybee Research Institute Jiangxi Agricultural University Nanchang China ARC Center of Excellence in Vision Science Research School of Biology The Australian National University Canberra ACT Australia

The honeybee （apis mellifera） is a social insect with strong sensory capacity and diverse behavioral repertoire and is recognized as a good model organism for studying the neurobiological basis of learning and memory. In this study, we analyzed the changes in microRNA （miRNA） and messenger RNA （mRNA） following maze-based visual learning using next-generation small RNA sequencing and Solexa/lllumina Digital Gene Expression tag profiling （DGE）. For small RNA sequencing, we obtained 13 367 770 and 13 132 655 clean tags from the maze and control groups, respectively. A total of 40 differentially expressed known miRNAs were detected between these two samples, and all of them were up-regulated in the maze group compared to the control group. For DGE, 5 681 320 and 5 939 855 clean tags were detected from the maze and control groups, respectively. There were a total of 388 differentially expressed genes between these two samples, with 45 genes up-regulated and 343 genes down-regulated in the maze group, compared to the control group. Additionally, the expression levels of 10 differentially expressed genes were confirmed by quantitative reverse transcription polymerase chain reaction （qRT-PCR） and the expression trends of eight of them were consistent with the DGE result, although the degree of change was lower in amplitude. The integrative analysis of miRNA and mRNA expression showed that, among the 40 differentially expressed known miRNAs and 388 differentially expressed genes, 60 pairs of miRNA/mRNA were identified as co-expressed in our present study. These results suggest that both miRNA and mRNA may play a pivotal role in the process of learning and memory in honeybees. Our sequencing data provide comprehensive miRNA and gene expression information for maze-based visual learning, which will facilitate understanding of the molecular mechanisms of honeybee learning and memory.

关键词： apis mellifera Y-maze learning and memory microRNA DGE differential expression

Evaluation of Morphometric Characters of Honeybee (apis mellifera L.) Populations in the Lake Chad Basin in Central Africa

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Advances in Entomology 2016年第2期4卷 75-89页

作者： Usman H. Dukku Department of Biological Sciences Abubakar Tafawa Balewa University Bauchi Nigeria

Samples of workers of honeybee were collected from 42 colonies in 13 localities in the Lake Chad Basin (parts of Cameroon, Chad, and Nigeria) and analysed using classical morphometry. Measurements of 35 morphological characters of body size, colour and pilosity were taken from 10 workers per colony and the data subjected to one-way analysis of variance (ANOVA), principal components analysis (PCA), hierarchical cluster analysis (HCA), stepwise discriminant analysis (DA) and Pearson’s product-moment correlation analysis. A one-way ANOVA revealed that means of 21 of the morphometric characters differed significantly (p 0.05). The bees formed one cluster in a PCA. However, scatter plots of altitude against principal component 1 of PCA (loaded with characters of body size) revealed an increase of size of the bees along the gradient of the Lake Chad Basin. The coefficient of determination (R2) indicated that 88% and 77% of the variation in size might be explained by altitude in the southeastern and southwestern parts of the basin, respectively. Additionally, there was a very highly significant strong positive relationship between principal component 1 and altitude (r(30) = 0.618, p < 0.0005). Similarly, HCA and DA classified the colonies into three morphoclusters whose distribution closely followed the altitude of the area.

关键词： apis mellifera African Bees Morphometry Lake Chad

Cloning and Sequence Analysis of Carbonic Anhydrase-related Protein 10-like in apis mellifera

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Agricultural Biotechnology 2012年第4期1卷 46-48,53页

作者： Zhaoying LI Department of Biological Science and Technology Shaanxi Institute of Education

[ Objective ] This study aimed to clone and analyze the gene sequence encoding carbonic anhydrase-related protein lO-like （ CARP X） from apis mellif era. [Method] The cDNA sequence of CARPX gene was cloned through RT-PCR, and then analyzed with bioinformatic method. [Result] The full-length cDNA sequence of CARPX was 972 bp long and encoded 324 amino acid residues, including a signal peptide and two transmembrane domains. The predicted molecular mass was 37.1 kDa and the predicted isoeleetric point was 7.458. The CARP X from A. mellifera shared close relationship with proteins from apisflorae, Bombtas impatiens, Bombus terrestris, Nasonia vitripennis and Acyrthosiphon pisum. The insect CARP X family may include two subfamilies. [ Conclusion] The results pro- vide basis for studying CARPs family.

关键词： apis mellifera CARP X gene Cloning Sequence analysis Protein structure

Investigating the Regulation of Hypopharyngeal Gland Activity in Honeybees（apis mellifera carnica） Under Overwintering Conditions via Morphologic Analysis Combined with iTRAQ-Based Comparative Proteomics

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Investigating the Regulation of Hypopharyngeal Gland Activit...

The comparison of digital gene expression tag profiling between nurses and foragers of apis mellifera

21世纪第三届全国蜂业科技与海峡两岸蜂产业发展大会暨首...

作者： Kang Wang Ting Ji Zheng-Guo Liu Yangzhou University Shandong University

The management of overwintering honeybee colonies is pivotal for the apiculture industry in *** investigate the molecular mechanisms involved in the seasonal regulation of the development of apis mellifera carnica hypopharyngeal gland（HG）,transmission electron microscopy（TEM） and large-scale proteomics（iTRAQ） were employed.A total of 3,025 proteins were identified at two-time points:winter（constituting group A） and early spring（constituting group B）.Among them,24 proteins were quantified and compared to identify their differential expression *** TEM profiles and iTRAQ analysis results might aid in understanding the molecular mechanisms associated with the seasonal changes in honeybee *** example,the acinar cells of spring worker honeybees had more mature ***,the ribosomal structures and protein biosynthesis were ***,the amount of exocrine royal jelly（RJ） produced could not have satisfied the demand of the feeding larvae and *** might suffer reduced immune function during their feeding *** present study elucidates the regulatory mechanisms of HG on overwintering and spring arousal in *** results might provide a practical and theoretical guideline for the overwintering management program of the apicultural industry.

关键词： apis mellifera overwintering hypopharyngeal gland morphology proteomics differential expression

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The comparison of digital gene expression tag profiling betw...

Identification of the proteome complement of hypopharyngeal glands from two strains of honeybees（apis mellifera）

第四届全国生物信息学与系统生物学学术大会

作者：刘芳李文峰李志国张少吾陈盛禄苏松坤杭州浙江大学动物科学学院澳大利亚国立大学环境医学生物学院生物科学研究所

The honey bee is a highly social insect acknowledged as one of the model organisms to study the mechanisms and evolution of social ***,there were still large unknown on the molecular basis controlling these complicate... 详细信息

关键词： apis mellifera Digital Gene Expression tag profiling(DGE) Differential expression Illumina sequencing Honey bee

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Identification of the proteome complement of hypopharyngeal ...

热带地区小蜜蜂(apis florea)和西方蜜蜂(apis mellifera)的种间竞争

中国养蜂学会蜜蜂饲养管理专业委员会第十三次学术研讨会

作者： Jianke Li~(a,*)Mao Fenga Zhaohui Zhang~b Yinghong Pan~b a Institute of Apicultural Research,Chinese Academy of Agricultural Science,Beijing 100093,China b Institute of Crop Science,Chinese Academy of Agricultural Sciences,The National Key Facility for Crop Gene Resources and Genetic Improvement,12 Zhongguancun Nandajie,Beijing 100081,China

＜正＞The protein complement of the hypopharyngeal gland（HG）of winter worker bees from a strain of apis mellifera artificially selected for increased royal jelly yield and Carnica winter worker bees was *** were partially identified using two-dimensional gel electrophoresis（2-DE）.MALDI-TOF MS and protein engine identification tools that were utilized for the honeybee *** identified proteins in the two bees strains were assigned to major royal jelly（RJ）proteins（MRJPs）.Marked differences were found in the heterogeneity of the MRJPs,in particular *** of the proteins,α-glucosidase and glucose oxidase,were related to carbohydrate metabolism and *** the first time in the HG of honeybees,two proteins, peroxiredoxin and thioredoxin peroxidase,which are related to antioxidation functions,and actin 5C,a major cytoskeletal actin protein which may supply enough actin for normal function of cells, have been *** suggest that the HGs serve a storage function in winter and that during winter the period the HG of high RJ producing bees store more proteins than those of Camica bees.

关键词： apis mellifera winter bees hypopharyngeal gland proteome

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中国蜂业 1977年第3期 31-35页

作者： N.科涅格尔周崧

前言关于利用生物学方法控制南美洲的“非洲蜂”与防止它通过中部美洲迁入美国的问题有过很多不同的争论。可是据我所知还没有人提出过利用最小的蜂种(apis florea)进行这项控制的方案。我对于美洲的情况没有什么亲身经验,“非洲蜂”对... 详细信息

前言关于利用生物学方法控制南美洲的“非洲蜂”与防止它通过中部美洲迁入美国的问题有过很多不同的争论。可是据我所知还没有人提出过利用最小的蜂种(apis florea)进行这项控制的方案。我对于美洲的情况没有什么亲身经验,“非洲蜂”对于美国的养蜂事业实际上是否构成严重的危险,对此我不愿意发表什么不同的看法。我们曾在Oberursel做了一个时

关键词：西方蜜蜂 apis florea apis mellifera 热带地区种间竞争种间斗争生存竞争