Investigating the Regulation of Hypopharyngeal Gland Activity in Honeybees（Apis mellifera carnica） Under Overwintering Conditions via Morphologic Analysis Combined with iTRAQ-Based Comparative Proteomics
作者单位：Yangzhou University Shandong University
会议名称：《21世纪第三届全国蜂业科技与海峡两岸蜂产业发展大会暨首...》
会议日期：2018年
学科分类：090504[农学-特种经济动物饲养（含：蚕、蜂等）] 0905[农学-畜牧学] 09[农学]
关 键 词：Apis mellifera overwintering hypopharyngeal gland morphology proteomics differential expression
摘 要：The management of overwintering honeybee colonies is pivotal for the apiculture industry in *** investigate the molecular mechanisms involved in the seasonal regulation of the development of Apis mellifera carnica hypopharyngeal gland（HG）,transmission electron microscopy（TEM） and large-scale proteomics（iTRAQ） were employed.A total of 3,025 proteins were identified at two-time points:winter（constituting group A） and early spring（constituting group B）.Among them,24 proteins were quantified and compared to identify their differential expression *** TEM profiles and iTRAQ analysis results might aid in understanding the molecular mechanisms associated with the seasonal changes in honeybee *** example,the acinar cells of spring worker honeybees had more mature ***,the ribosomal structures and protein biosynthesis were ***,the amount of exocrine royal jelly（RJ） produced could not have satisfied the demand of the feeding larvae and *** might suffer reduced immune function during their feeding *** present study elucidates the regulatory mechanisms of HG on overwintering and spring arousal in *** results might provide a practical and theoretical guideline for the overwintering management program of the apicultural industry.