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Inhibition of hepatitis B virus via selective apoptosis modulation by Chinese patent medicine Liuweiwuling Tablet

World Journal of Gastroenterology 2024年第13期30卷 1911-1925页

作者： Fei-Lin Ge Yan Yang Lan-Lan Si Yuan-Hua Li Meng-Zhen Cao Jun Wang Zhao-Fang Bai Zhi-Gang Ren Xiao-He Xiao Yan Liu Department of Chinese Medicine State Key Laboratory of Antiviral DrugsThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina The Fifth Medical Center of Chinese PLA General Hospital Beijing 100039China College of Traditional Chinese Medicine Chongqing Medical UniversityChongqing 400010China Beijing Key Laboratory of Emerging Infectious Diseases Peking University Ditan Teaching HospitalBeijing 100015China Department of Infectious Diseases State Key Laboratory of Antiviral Drugsthe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina

BACKGROUND Liuweiwuling Tablet(LWWL)is a Chinese patent medicine approved for the treatment of chronic inflammation caused by hepatitis B virus(HBV)*** studies have indicated an anti-HBV effect of LWWL,specifically in terms of antigen inhibition,but the underlying mechanism remains *** To investigate the potential mechanism of action of LWWL against *** In vitro experiments utilized three HBV-replicating and three non-HBV-replicating cell *** in vivo experiment involved a hydrodynamic injectionmediated mouse model with HBV *** and metabolomics were used to investigate the underlying mechanisms of action of *** In HepG2.1403F cells,LWWL(0.8 mg/mL)exhibited inhibitory effects on HBV DNA,hepatitis B surface antigen and pregenomic RNA(pgRNA)at rates of 51.36%,24.74%and 50.74%,*** inhibition rates of LWWL(0.8mg/mL)on pgRNA/covalently closed circular DNA in HepG2.1403F,HepG2.2.15 and HepG2.A64 cells were 47.78%,39.51%and 46.74%,*** of transcriptomics and metabolomics showed that the anti-HBV effect of LWWL was primarily linked to pathways related to apoptosis(PI3K-AKT,CASP8-CASP3 and P53 pathways).Apoptosis flow analysis revealed that the apoptosis rate in the LWWL-treated group was significantly higher than in the control group(CG)among HBV-replicating cell lines,including HepG2.2.15(2.92%±1.01%vs 6.68%±2.04%,P0.05),L02 cells(5.49%±0.80%vs 5.48%±1.01%,P>0.05)and LX2 cells(6.29%±1.54%vs 6.29%±0.88%,P>0.05).TUNEL staining revealed a significantly higher apoptosis rate in the LWWL-treated group than in the CG in the HBVreplicating mouse model,while no noticeable difference in apoptosis rates between the two groups

关键词： Hepatitis B virus Chinese patent medicine antiviral activity antiviral mechanism Selective apoptosis

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Studies on Antivirus Effects of Polysaccharides from Sargassum fusiforme in vitro

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有机化学 2004年第Z1期24卷 128-129页

作者： CEN,Ying-Zhou KHOO,Gaik-Ming WANG,Ling-Yun ZHANG,Ying Department of Chemistry Jinan University Guangzhou 510632 Department of Chemistry Jinan University Guangzhou 510632 Department of Chemistry Jinan University Guangzhou 510632 Department of Chemistry Jinan University Guangzhou 510632

　　To assay antiviral effects of the polysaccharide and its separated products from Sargassumfusiforme on HSV-1 and CVB3 and their antiviral mechanism, the antiviral effects of all samples on HSV-1 and CVB3 and their cytotoxicity on vero cells were studied by CPE and MTT method. antiviral mechanism of two samples was briefly studied by four different ways. The cytotoxicity of samples on vero cells was not detected (CC＞5000 μg/mL). All samples except PSJ had obviously HSV-1 inhibitory effect. antiviral activities of them were increasingly strengthened with raising their *** activities of F1, F2 and F4 were better than that of ACV, and antiviral activities of all samples on CVB3 were better than ribavirin injection. Fi, F2 and F4 had remarkable anti-CVB3 activity. The experiment showed that polysaccharide was able to not only kill the above viruses directly but also restrain them by getting into cell or absorbing on cells.……

关键词： antiviral mechanism vero cells inhibitory effect MTT method

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Studies on the antiviral Activities in vitro of Polysaccharide from Eucheuma striatum

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有机化学 2004年第Z1期24卷 126-127页

作者： CEN,Ying-Zhou KHOO,Gaik-Ming YE,Shao-Ming RUI,Wen Department of Chemistry Jinan University Guangzhou 510632 Department of Chemistry Jinan University Guangzhou 510632 Department of Chemistry Jinan University Guangzhou 510632 Department of Chemistry Jinan University Guangzhou 510632

　　To assay the antiviral activities on HSV-1 and CVB3 in vitro of the polysaccharide from Eucheuma striatum, its antiviral mechanism was explored. Vero cells were infected by HSV-1 and CVB3, and they were cultured with serial dilutions of polysaccharide. The cells cytotoxicity of Polysaccharide was evaluated by the MTT method. The inhibitory effects were evaluated by the cytopathic effect (CPE). Its antiviral mechanism was researched by the method of giving samples in different time. The polysaccharide could inhibit the CPE of cells infected by HSV-1 and CVB3. It showed low cytotoxicity on vero cells. Its antiviral activities were better than those of acyclovir and ribavirin which were run in parallel as the positive control samples. The polysaccharide from Eucheuma striatum has potent antiviral activities. Its antiviral mechanism is that it can prevent the virus from absorbing to the cell surface.……

关键词： antiviral mechanism vero cells inhibitory effects cytopathic effect control samples MTT method method of in vitro

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Spider antiviral peptide targets NS2B-NS3 protease of Flaviviruses

Spider antiviral peptide targets NS2B-NS3 protease of Flaviv...

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第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存...

作者： Mengyao Ji Meichen Xing Ren Lai College of Life Sciences Nanjing Agricultural University Key Laboratory of Animal Models and Human Disease mechanisms of Chinese Academy of Sciences Key Laboratory of Bioactive Peptides of Yunnan Province Kunming Institute of ZoologyChinese Academy of Sciences

Flaviviruses,such as Dengue,Japanese encephalitis,Tick-borne encephalitis,West Nile,Yellow Fever,and Zika virus,are single-stranded RNA viruses and predominantly transmitted by the widely distributed Aedes mosquitoes in *** important human pathogens,the geographic reach of Flaviviruses is increasing and they have become threats to public health worldwide and currently there is no approved specific drug for *** vaccination is the primary strategy for preventing Flaviviruses infections,antivirals may also play an important role in controlling their infection and *** recent years,development of peptide antivirals has gained much *** animal host defense peptides（HDPs） which uniquely evolved to protect the hosts have been shown to have antiviral *** this study,we collected the spider venom of Alopecosa nagpag spider from the Napa sea area of the Shangri-La,Yunnan *** anti-Dengue serotype-2 virus and anti-Zika virus HDP named GY-36 was identified from the spider ***-36 functions as a NS2 B-NS3 protease inhibitor and its anti-flavivirus activity was verified in U251,A549 and RAW264.7 cells by qPCR,plaque assay,western blot and immunofluorescence ***,GY-36 administration in Ifnar～（-/-） mice inhibited Dengue and Zika virus ***,our findings not only demonstrate that GY-36 might be a candidate for antiviral drug,but also indicated spider HDPs as antiviral precursor molecules.

关键词： Alopecosa nagpag Dengue virus Zika virus host defense peptide antiviral mechanism Flaviviruses

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A scorpion venom peptide Ev37 restricts viral late entry by alkalizing acidic organelles

A scorpion venom peptide Ev37 restricts viral late entry by ...

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第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存...

作者： Fangfang Li Yange Lang Zhenglin Ji Zhiqiang Xia Yuewen Han Yuting Cheng Gaomin Liu Fang Sun Yonghui Zhao Minjun Gao Zongyun Chen Yingliang Wu Wenxin Li Zhijian Cao

Viral infection still threatens human health all over the world,and many people die from viral diseases every ***,there are no effective vaccines or drugs for preventing or managing most viral ***,the discovery and development of broad-spectrum antiviral agents remain ***,we expressed and purified a scorpion venom peptide,Ev37,from the scorpion Euscorpiops validus in a prokaryotic *** found that rEv37 can inhibit dengue virus type 2（DENV-2）,hepatitis C virus（HCV）,Zika virus（ZIKV） and herpes simplex virus type 1（HSV-1）infections in a dose-dependent manner at noncytotoxic concentrations,but that it has no effect on Sendai virus（SeV） and Adenovirus（AdV） infections in ***,rEv37 alkalized acidic organelles to prevent low pH-dependent fusion of the viral membrane-endosomal membrane,which mainly blocks the release of the viral genome from the endosome to the cytoplasm and then restricts viral late *** together,our results indicate that the scorpion venom peptide Ev37 is a broadspectrum antiviral agent with a specific molecular mechanism against viruses undergoing low pHdependent fusion activation during entry into host *** conclude that Ev37 is a potential candidate for development as an antiviral drug.

关键词： scorpion venom peptide antiviral peptide antiviral mechanism dengue virus(DENV) hepatitis C virus(HCV) Zika virus(ZIKV) herpes simplex virus(HSV) viral entry membrane fusion endosome

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