Spider antiviral peptide targets NS2B-NS3 protease of Flaviviruses
作者单位：College of Life SciencesNanjing Agricultural University Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences Key Laboratory of Bioactive Peptides of Yunnan ProvinceKunming Institute of ZoologyChinese Academy of Sciences
会议名称：《第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存适应到转化医学专题学术会议》
会议日期：2019年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Alopecosa nagpag Dengue virus Zika virus host defense peptide antiviral mechanism Flaviviruses
摘 要：Flaviviruses,such as Dengue,Japanese encephalitis,Tick-borne encephalitis,West Nile,Yellow Fever,and Zika virus,are single-stranded RNA viruses and predominantly transmitted by the widely distributed Aedes mosquitoes in *** important human pathogens,the geographic reach of Flaviviruses is increasing and they have become threats to public health worldwide and currently there is no approved specific drug for *** vaccination is the primary strategy for preventing Flaviviruses infections,antivirals may also play an important role in controlling their infection and *** recent years,development of peptide antivirals has gained much *** animal host defense peptides(HDPs) which uniquely evolved to protect the hosts have been shown to have antiviral *** this study,we collected the spider venom of Alopecosa nagpag spider from the Napa sea area of the Shangri-La,Yunnan *** anti-Dengue serotype-2 virus and anti-Zika virus HDP named GY-36 was identified from the spider ***-36 functions as a NS2 B-NS3 protease inhibitor and its anti-flavivirus activity was verified in U251,A549 and RAW264.7 cells by qPCR,plaque assay,western blot and immunofluorescence ***,GY-36 administration in Ifnar～(-/-) mice inhibited Dengue and Zika virus ***,our findings not only demonstrate that GY-36 might be a candidate for antiviral drug,but also indicated spider HDPs as antiviral precursor molecules.