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Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats

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Journal of Integrative Medicine 2022年第4期20卷 348-354页

作者： Talat Albukhari Bassem Refaat El-Sayed Bakr Sameh Baz Bodour Rajab Hossam Gadalla Mohamed El-Boshy Department of Immunology and Hematology Faculty of MedicineUmm Al-Qura UniversityMakkah 21955Saudi Arabia Department of Laboratory Medicine Faculty of Applied Medical SciencesUmm Al-Qura UniversityMakkah 7607Saudi Arabia Clinical Nutrition Department Faculty of Applied Medical Sciences Umm Al-Qura UniversityMakkah 7607Soudi Arabia Clinical Pathology Department Faculty of Veterinary MedicineMansoura UniversityMansoura 35516Egypt

Objective: Salvadora persica(SP) is used as a food additive and is a common ingredient in folk *** study investigates the antioxidant, anti-inflammatory, and beneficial effects of SP against cyclophosphamide(CYP) toxicity in ***: In a 10-day study, 32 male rats were equally allocated into 4 groups(8 rats/group) as follows:the normal control(NC group), normal rats that only received oral aqueous extract of SP(100 mg/[kg·d];SP group), animals treated with intraperitoneal CYP injections(30 mg/[kg·d];CYP group), and the CYP + SP group that concurrently received CYP with SP aqueous extract. Serum samples were collected to measure the liver and renal biochemical profiles, as well as antioxidant and oxidative stress markers and the concentrations of interleukin-1β(IL-1β), IL-6, IL-10, tumor necrosis factor-a(TNF-a), nuclear factor-κB(NF-κB) and adenosine 5’-monophosphate-activated protein kinase(ampK). Hepatic and renal tissues were also harvested for histopathology and to measure apoptosis using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling technique, alongside tissue levels of oxidative stress ***: Liver enzymes, total bilirubin, creatinine and urea, as well as serum IL-1β, IL-6, TNF-a and NF-κB increased significantly, whilst total protein, albumin, calcium, IL-10 and ampK declined in serum of the CYP group relative to the NC group. The hepatorenal concentrations of glutathione, glutathione peroxidase and catalase declined markedly in the CYP group, whereas malondialdehyde, protein adducts, and apoptosis index increased compared with the NC group. By contrast, the hepatorenal biochemistry and apoptosis index of the SP group were comparable to the NC group. Interestingly, the CYP + SP group had significant improvements in the liver and renal biochemical parameters, enhanced anti-oxidative and anti-inflammatory effects, and marked declines in hepatic and renal apoptosis relative to the CYP group. Moreover, all mon

关键词： Cyclophosphamide Salvadora persica Cytokine NF-κB amp-activated protein kinases Antioxidants Anti-inflammatory agents

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Efficacy of aqueous extract of flower of Edgeworthia gardneri(Wall.)Meisn on glucose and lipid metabolism in KK/Upj-Ay/J mice

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Journal of Traditional Chinese Medicine 2022年第2期42卷 187-193页

作者： ZHANG Chengfei QIN Lingling WANG Haiyan SUN Boju ZHAO Dan ZHANG Qiue ZHONG Fengying WU Lili LIU Tonghua School of Life Sciences Beijing University of Chinese MedicineBeijing 102488China Technology Department Beijing University of Chinese MedicineBeijing 100029China Dongfang Hospital of Beijing University of Chinese Medicine Beijing 100029China Key Laboratory of TCM Health Cultivation of Beijing Beijing University of Chinese MedicineBeijing 102488China the First Clinical Medical College of Shaanxi University of Traditional Chinese Medicine Shaanxi 712000China

OBJECTIVE:To observe the effects of the flower of Edgeworthia gardneri(Wall.)Meisn(EWM)on glucose and lipid metabolism in KK/upj-Ay/J(KKAy)mice and investigate the possible mechanism of EWM in the liver of KKAy mice by transcriptome ***:Forty KKAy mice were fed a high-sugar and high-fat diet for 3 weeks to establish the animal model of metabolic *** 5 weeks of continuous administration of EWM,serum high-density lipoprotein(HDL),low-density lipoprotein(LDL),triglycerides(TG),total cholesterol(TC),and free fatty acids(FFA)were detected by *** fasting insulin(Fins)and adiponectin levels were measured by enzymelinked immunosorbent *** tissue fixed with paraformaldehyde was stained with hematoxylineosin and oil red *** analysis was used to evaluate the liver *** expressions of lipoprotein lipase(LPL),peroxisome proliferator-activated receptor-γ(PPARγ),adenosine 5‘-monophosphate-activated protein kinase(ampK),sterol regulatory element binding protein1c(SREBP-1c),and fatty acid synthase(Fas)mRNA and protein in liver tissue were detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blot ***:EWM slightly reduced FBG and Fins in KKAy ***,EWM was able to downregulate serum LDL,TG,TC,and FFA and upregulate the expression of serum HDL and *** analysis revealed the following differential pathways:the peroxisome proliferator-activated receptor(PPAR)signaling pathway and the ampK signaling ***-PCR and western blot analysis detected the associated genes and *** addition,EWM was able to upregulate the expression of ampK and downregulate the expression of PPARγ,SREBP1c,and Fas mRNA and protein and upregulate the expression of LPL ***:EWM can alleviate lipid metabolism disorders and to some extent improve glucose metabolism disorders in KKAy *** effects may be related to regulating PPARγ/LPL and activating the

关键词： transcriptome PPAR gamma amp-activated protein kinases Edgeworthia gardneri(Wall.)Meisn flower

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Effect of Yiqihuoxue prescription on myocardial energy metabolism after myocardial infarction via cross talk of liver kinase B1-depen-dent Notch1 and adenosine 5'-monophosphate-activated protein kinase

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Journal of Traditional Chinese Medicine 2017年第3期37卷 378-386页

作者： Wu Jiangong Chen Xi Guo Shuwen Liu Wenchen Zhang Lu Li Fanghe Wu Jiani Huang Xiaolou Cai Qian Tan Xiaobo Wang Hui Institute of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 100029China

OBJECTIVE: To investigate the effect of Yiqihuoxue prescription(YQHX) from Traditional Chinese Medicine(TCM) on myocardial glucose and lipid metabolism after myocardial infarction via the cross talk between the liver kinase B1(LKB1)-dependent Notch1 and adenosine 5'-monophosphate(amp)-activated protein kinase(ampK). YQHX was prepared with substances with properties that benefit, to activate blood circulation based on the TCM ***: Animal models of myocardial infarction were established by ligating Sprague Dawley rats' left anterior descending coronary arteries. The animals were randomly divided into a myocardial infarction(MI) group, a YQHX group, a perindopril group, a γ-secretase inhibitor, Notch signal inhibitor(DAPT) group, a DAPT+YQHX group and a sham group. The related drugs were administered on the second day after operation, and changes in the relevant indexes were examined on weeks 1 and *** in cardiac structure and function were examined by echocardiography. The glucose and free fatty acids(FFA) were examined by ELISA. The expression of Notch, LKB1 and ampK m RNA was examined by a real-time fluorescence quantitative method. The expression of glucose transporter 4(GLUT4), and the expression of total acetyl-Co A carboxylase(ACC) and its phosphorylation were examined by western ***: Compared with the sham group, the expression of Notch, LKB1 and ampK m RNA in the MI group was lower. Compared with the MI group, the expression of these m RNAs in the YQHX and perindopril groups was higher, and their expression in the DAPT group was lower. At all time points, the protein expression of GLUT4 and p ACC decreased in the MI group. On week 1, the expression of p ACC protein was higher. In the DAPT group, the expression of p ACC protein decreased. Compared with the YQHX group, the expression of p ACC protein in the DAPT + YQHX group was lower. On week 4,compared with the MI group, the expression of GLUT4 protein in the YQHX group and the pe

关键词： Myocardial infarction Reinforcing Qi ac-tivating blood Lipid metabolism Receptor, Notch1 amp-activated protein kinases

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Ginsenoside Rb1 alleviates chronic intermittent hypoxia-induced diabetic cardiomyopathy in db/db mice by regulating the adenosine monophosphate-activated protein kinase/Nrf2/heme oxygenase-1 signaling pathway

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Journal of Traditional Chinese Medicine 2023年第5期43卷 906-914页

作者： LIU Bingbing LI Jieru SI Jianchao CHEN Qi YANG Shengchang JI Ensheng Department of Physiology Hebei University of Chinese MedicineShijiazhuang 050200China Hebei Technology Innovation Center of TCM Combined Hydrogen Medicine Shijiazhuang 050200China

OBJECTIVE:To examine the protective effect of ginsenoside Rb1(Rb1),the main component of Renshen(Radix Ginseng),on cardiomyopathy in db/db mice exposed to chronic intermittent hypoxia(CIH)and explore the potential underlying mechanism of Rb1 in treating diabetic cardiomyopathy(DCM).METHODS:The db/db mice were randomly separated into five groups:normal control group,model group,Rb120 mg/kg group,Rb140 mg/kg group,and glucagon-like peptide-1(GLP-1)*** were exposed to aircondition or CIH for 8 weeks,and Rb1 and GLP-1 were administrated before CIH exposure every *** glucose tolerance test(OGTT),intraperitoneal insulin tolerance test(IPITT),total cholesterol(TC),triglyceride(TG),and high-density lipoprotein cholesterol(HDL-C)were detected to evaluate glycolipid *** level of insulin was detected by a mouse enzyme-linked immunosorbent assay(ELISA).Cardiac function was detected by echocardiography,and myocardial pathology was observed by hematoxylin-eosin and Masson *** expression of collagenⅠand collagenⅢwas detected by *** monophosphate-activated protein kinase(ampK)/Nrf2/heme oxygenase-1(HO-1)signaling pathway was detected by Western blot and ***:Rb1 treatment could improve glucose tolerance and the level of cardiac function indexes,and inhibit the level of oxidative stress indexes and the expression of collagenⅠand collagenⅢ.Moreover,Rb1 treatment enhanced ampK phosphorylation and increased Nrf2 and HO-1 ***:Rb1 treatment alleviated CIH-induced diabetic cardiomyopathy and glycolipid metabolism disorders in db/db mice by inhibiting oxidative stress and regulating the ampK/Nrf2/HO-1 signaling pathway.

关键词： ginsenosides diabetic cardiomyopathies oxidative stress amp-activated protein kinases heme oxygenase-1 signal transduction chronic intermittent hypoxia

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Efficacy of Sijunzi decoction(四君子汤) on limb weakness in spleen Qi deficiency model rats through adenosine monophosphate-activated protein kinase/unc-51 like autophagy activating kinase 1 signaling

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Journal of Traditional Chinese Medicine 2021年第4期41卷 617-623页

作者： LIU Wenjun XU Xinzhu DUAN Zhiyuan LIANG Xicai MA Dan LI Gege XIE Xin CHAI Jiyan CHEN Jing SHAN Dehong Teaching and Experimental Center Liaoning University of Traditional Chinese MedicineShenyang 110847China College of Integrated Traditional and Western Medicine Liaoning University of Traditional Chinese MedicineShenyang 110847China Animal Laboratory Center Liaoning University of Traditional Chinese MedicineShenyang 110847China College of Acupuncture Liaoning University of Traditional Chinese MedicineShenyang 110847China

OBJECTIVE:To investigate the efficacy of Sijunzi decoction(四君子汤)on limb weakness in a rat model of spleen Qi deficiency(SQD),and to study its effect on mitophagy in skeletal muscle through adenosine monophosphate-activated protein kinase(ampK)/unc-51 like autophagy activating kinase 1(ULK1)***:SQD model rats were produced by fasting combined with forced swimming method for15 *** model assessment,rats were randomly divided into four groups of 10[low/middle/high(L/M/H)Sijunzi decoction dose groups and a normal saline(S)group].Limb holding power(HP)and body mass(BM)were measured after 2 weeks of *** euthanasia,quadriceps femoris were dissected and myofiber and mitochondrial morphology were observed by transmission electron microscopy(TEM).Mitochondrial membrane potential(MMP),adenosine triphosphatase(ATP)and reactive oxygen species(ROS)levels were determined using colorimetric methods,and immunoblot analysis of Microtubule-associated protein light chain 3(LC3)and Sequestosome 1(p62)was performed to monitor mitophagy and ampK/ULK1 ***:Compared with control(C)group rats,in the S group,HP was reduced,the myofiber Z line was disordered,mitochondria were scattered,and numerous vacuoles and mitophagy were *** and ATP levels were reduced,ROS levels were elevated,and LC3B expression,and p-ampKα(Thr172)/ampKα,p-ULK1(Ser555)/ULK1,and p-Raptor(Ser792)/Raptor ratios were increased,while p62 expression and p-mT OR(Ser2448)/mT OR and p-ULK1(Ser757)/ULK1 ratios were *** treatment,compared with the S group,HP was improved in M and H groups but not in the L *** was reduced in M,H and L groups but the Z line was disordered and vacuolization remained in the L *** levels were elevated in M,H and L groups,and MMPs were elevated in M and H groups but not in the L *** levels were decreased in M,H and L groups,as were LC3B expression and p-Raptor(Ser792)/Raptor ratios,while p62 expression and p-mT OR(

关键词： spleen-Qi deficiency muscle skeletal mitochondria oxidative phosphorylation mitophagy amp-activated protein kinases autophagy-related protein-1 homolog Sijunzi decoction

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Anti-hypertensive and endothelia protective effects of Fufang Qima capsule(复方芪麻胶囊) on primary hypertension via adiponectin/adenosine monophosphate activated protein kinase pathway

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Journal of Traditional Chinese Medicine 2021年第6期41卷 919-926页

作者： ZHAO Zhiyue SHI Zhenyu ZHANG Zhenzhen LI Yinghong ZENG Xiaohui CHEN Yuxing YAO Nan ZHOU Min SU Hui WANG Qinghai JIN LiLi Department of Cardiovascular The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine(Guangdong Second Traditional Chinese Medicine Hospital)Guangzhou 510095China Department of the Electrocardiogram The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine(Guangdong Second Traditional Chinese Medicine Hospital)Guangzhou 510095China Department of Science and Education The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine(Guangdong Second Traditional Chinese Medicine Hospital)Guangzhou 510095China Pharmacological Laboratory The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine(Guangdong Second Traditional Chinese Medicine Hospital)Guangzhou 510095China Department of Emergency The Fifth Clinical Medical College of Guangzhou University of Chinese Medicine(Guangdong Second Traditional Chinese Medicine Hospital)Guangzhou 510095China

OBJECTIVE:To investigate the potential mechanism of the vascular remodeling effect and provide additional information about anti-hypertension activity of Fufang Qima capsule(复方芪麻胶囊,QM).METHODS:Spontaneous hypertensive rats(SHRs)were used to study the underlying mechanism of the anti-hypertension activity of *** this study,SHRs were randomly divided into 5 groups:model group,Telmisartan group(7.2 mg/kg,p.o.),and three QM groups(0.9298,1.8596,and 3.7192 g/kg,p.o.).Wistar Kyoto rats(WKY)were used as normal control *** pressure(BP),aorta,perivascular adipose tissue(PVAT)histology were investigated to evaluate the effect of *** oxide(NO)and endothelial nitric oxide synthase(eNOS)phosphorylation were ***(APN)secretion,as well as APN signal pathway proteins including APN,adiponectin receptors(R1 and R2)and adenosine 5’-monophosphate-activated protein kinase(ampK)were all ***:QM significantly reduced BP and ameliorated the vascular pathological change,*** media thicken and collagen fiber ***,QM increased concentration of NO and the phosphorylation of eNOS in the *** anti-hypertensive and endothelia-protective effect of QM could be attributed to activating APN/ampK pathway by up-regulating the expression of APN in PVAT and APN Receptor 2,ampKαand phosphorylated ampKαin the ***:The QM alleviation effect mechanism for primary hypertension was via modulating the APN/ampK signal pathway.

关键词： hypertension adiponectin amp-activated protein kinases nitric oxide synthase typeⅢ Fufang Qima capsule

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Study on the regulatory effect of herbal cake-partitioned moxibustion on colonic CD206,ampK and TSC2 in rats with Crohn disease

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Journal of Acupuncture and Tuina Science 2021年第5期19卷 329-337页

作者： Dong Xiao-qing Li Xiao-ying Wang Xue-jun Guo Xiao-cong Long Jun-yi Lu Yun-qiong Liu Li Caoyao Jia-ni Zhang Dan Lu Yuan Wu Huan-gan Xie Chen Ma Xiao-peng Yang Yan-ping Shanghai University of Traditional Chinese Medicine Shanghai 201203China Xi'an Traditional Chinese Medicine Hospital of Encephalopathy Shaanxi ProvinceXi'an 710032China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030China 不详

Objective:To explore the mechanism of herbal cake-partitioned moxibustion in Crohn disease(CD)treatment by observing the effect of herbal cake-partitioned moxibustion on protein expressions of colonic M2 macrophage marker CD206,amp-activated protein kinase(ampK)and tuberous sclerosis complex(TSC)***:Twenty-six specific pathogen free male rats were randomly divided into a normal group,a model group and a herbal cake-partitioned moxibustion *** CD model was prepared by enema with the mixture of 5%(W/V)2,4,6-trinitrobenzene sulfonic acid(TNBS)and 50%ethanol at 2:1(volume ratio).After the model was successfully prepared,rats in the herbal cake-partitioned moxibustion group received herbal cake-partitioned moxibustion at Qihai(CV 6)and bilateral Tianshu(ST 25).Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat colon;immunohistochemical technique was used to detect the expression of colonic CD206 protein;Western blot,immuno fluorescence,and real-time fluorescence quantitative polymerase chain reaction(RTqPCR)technologies were used to detect the protein and mRNA expressions of colonic ampK and ***:Compared with the normal group,rats in the model group showed damaged colonic mucosa,missing of the epithelial layer;thickened submucosa,vascular proliferation,massive infiltration of monocytes and lymphocytes,and cracked ulcers that reached the muscle *** in the herbal cake-partitioned moxibustion group showed reduced intestinal inflammation and healing intestinal epithelium *** with the normal group,rat colonic CD206 protein expression,and the protein and mRNA expressions of colonic ampK and TSC2 were decreased in the model group(all Pprotein and mRNA expressions of ampK and TSC2 in the herbal cake-partitioned moxibustion(all P<0.05).Conclusion:Herbal cake-partitioned moxibustion can reduce i

关键词： Moxibustion Therapy Indirect Moxibustion Medicinal Cake-partitioned Moxibustion Point,Tianshu(ST 25) Point,Qihai(CV 6) Crohn Disease amp-activated protein kinases Tuberous Sclerosis Complex 2 protein

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Study on the relationship between relieving energy crisis in myofascial trigger points with An-Pressing manipulation and ampK/PGC-1α pathway activation

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Journal of Acupuncture and Tuina Science 2022年第4期20卷 257-264页

作者： KUANG Xiaoxia LI Wu JIANG Quanrui WEI Wei LI Tielang LI Jiangshan YANG Yanping Hunan University of Chinese Medicine Changsha 410208China The Second Hospital University of South ChinaHengyang 421000China 不详

Objective To explore the mechanism of An-Pressing manipulation in relieving energy crisis in chronic myofascial trigger points(MTrPs)by observing the effects of An-Pressing manipulation on adenosine triphosphate(ATP),adenosine 5′-monophosphate(amp)-activated protein kinase(ampK)/peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α)pathway and mitochondrial ultrastructure of skeletal muscle cells in MTrPs *** Forty-eight male Sprague-Dawley rats were randomly divided into a blank group,a model group,a lidocaine group,and an An-Pressing manipulation group,with 12 rats in each *** model group,lidocaine group and An-Pressing manipulation group were used to replicate the MTrPs rat model by blunt shock and centrifugal motion *** modeling,the An-Pressing manipulation group was subjected to 7 times An-Pressing manipulation,once every other day;the lidocaine group was treated with 3 times of injection of lidocaine at the MTrPs,once every 6 *** blank group and the model group were fed normally without *** the intervention,local muscle tissue was taken to detect the content of ATP and the expression of ampK,phosphorylated ampK(phospho-ampK),PGC-1α,and glucose transporter 4(GluT4),and the ultrastructure of mitochondria was observed under an electron *** Compared with the blank group,the ATP content in the model group was decreased(Pprotein expression levels of phospho-ampK,PGC-1α,and GluT4 and the ratio of phospho-ampK to ampK were decreased(Pprotein expression levels of phospho-ampK,PGC-1α,and GluT4 and the ratio of phospho-ampK to ampK were increased(P<0.05);under the electron microscope,the number of mitochondria increased,t

关键词： Tuina Massage An-Pressing Manipulation Myofascial Trigger Point Energy Metabolism amp-activated protein kinases Peroxisome Proliferator-activated Receptor Gamma Coactivator 1-α Signal Transduction

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