Study on the relationship between relieving energy crisis in myofascial trigger points with An-Pressing manipulation and AMPK/PGC-1α pathway activation

作     者：KUANG Xiaoxia LI Wu JIANG Quanrui WEI Wei LI Tielang LI Jiangshan YANG Yanping 匡小霞;李武;蒋全睿;危威;李铁浪;李江山;杨燕萍(翻译)

作者机构：Hunan University of Chinese MedicineChangsha 410208China The Second HospitalUniversity of South ChinaHengyang 421000China 不详 

出 版 物：《Journal of Acupuncture and Tuina Science》 (针灸推拿医学（英文版）)

年 卷 期：2022年第20卷第4期

页      面：257-264页

核心收录：

学科分类：1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：国家自然科学基金项目,No.81973975 湖南省自然科学青年基金项目No.2021JJ40482 湖南省教育厅优青项目,No.20B439 衡阳市科技指导性项目,No.2019JH011041 湖南中医药大学附属第一医院湖南省院士专家工作站(石学敏)开放基金No.201901 

主　　题：Tuina Massage An-Pressing Manipulation Myofascial Trigger Point Energy Metabolism AMP-Activated Protein Kinases Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-α Signal Transduction 

摘      要：Objective To explore the mechanism of An-Pressing manipulation in relieving energy crisis in chronic myofascial trigger points(MTrPs)by observing the effects of An-Pressing manipulation on adenosine triphosphate(ATP),adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)/peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α)pathway and mitochondrial ultrastructure of skeletal muscle cells in MTrPs *** Forty-eight male Sprague-Dawley rats were randomly divided into a blank group,a model group,a lidocaine group,and an An-Pressing manipulation group,with 12 rats in each *** model group,lidocaine group and An-Pressing manipulation group were used to replicate the MTrPs rat model by blunt shock and centrifugal motion *** modeling,the An-Pressing manipulation group was subjected to 7 times An-Pressing manipulation,once every other day;the lidocaine group was treated with 3 times of injection of lidocaine at the MTrPs,once every 6 *** blank group and the model group were fed normally without *** the intervention,local muscle tissue was taken to detect the content of ATP and the expression of AMPK,phosphorylated AMPK(phospho-AMPK),PGC-1α,and glucose transporter 4(GluT4),and the ultrastructure of mitochondria was observed under an electron *** Compared with the blank group,the ATP content in the model group was decreased(P0.05),the protein expression levels of phospho-AMPK,PGC-1α,and GluT4 and the ratio of phospho-AMPK to AMPK were decreased(P0.05);under the electron microscope,the number of mitochondria decreased,and they were deformed,small in volume,and had deformed *** with the model group,the ATP contents in the An-Pressing manipulation group and the lidocaine group were increased(P0.05),and the protein expression levels of phospho-AMPK,PGC-1α,and GluT4 and the ratio of phospho-AMPK to AMPK were increased(P0.05);under the electron microscope,the number of mitochondria increased,t