Efficacy of aqueous extract of flower of Edgeworthia gardneri(Wall.)Meisn on glucose and lipid metabolism in KK/Upj-Ay/J mice

作     者：ZHANG Chengfei QIN Lingling WANG Haiyan SUN Boju ZHAO Dan ZHANG Qiue ZHONG Fengying WU Lili LIU Tonghua ZHANG Chengfei;QIN Lingling;WANG Haiyan;SUN Boju;ZHAO Dan;ZHANG Qiue;ZHONG Fengying;WU Lili;LIU Tonghua

作者机构：School of Life SciencesBeijing University of Chinese MedicineBeijing 102488China Technology DepartmentBeijing University of Chinese MedicineBeijing 100029China Dongfang Hospital of Beijing University of Chinese MedicineBeijing 100029China Key Laboratory of TCM Health Cultivation of BeijingBeijing University of Chinese MedicineBeijing 102488China the First Clinical Medical College of Shaanxi University of Traditional Chinese MedicineShaanxi 712000China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第2期

页      面：187-193页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Key Laboratory of TCM Health Cultivation of Beijing(grant No.BZ0259) the Creation and Talent Introduction Base of Prevention and Treatment of Diabetes and Its Complications with Traditional Chinese Medicine(No.B20055) 

主　　题：transcriptome PPAR gamma AMP-activated protein kinases Edgeworthia gardneri(Wall.)Meisn flower 

摘      要：OBJECTIVE:To observe the effects of the flower of Edgeworthia gardneri(Wall.)Meisn(EWM)on glucose and lipid metabolism in KK/upj-Ay/J(KKAy)mice and investigate the possible mechanism of EWM in the liver of KKAy mice by transcriptome ***:Forty KKAy mice were fed a high-sugar and high-fat diet for 3 weeks to establish the animal model of metabolic *** 5 weeks of continuous administration of EWM,serum high-density lipoprotein(HDL),low-density lipoprotein(LDL),triglycerides(TG),total cholesterol(TC),and free fatty acids(FFA)were detected by *** fasting insulin(Fins)and adiponectin levels were measured by enzymelinked immunosorbent *** tissue fixed with paraformaldehyde was stained with hematoxylineosin and oil red *** analysis was used to evaluate the liver *** expressions of lipoprotein lipase(LPL),peroxisome proliferator-activated receptor-γ(PPARγ),adenosine 5‘-monophosphate-activated protein kinase(AMPK),sterol regulatory element binding protein1c(SREBP-1c),and fatty acid synthase(Fas)mRNA and protein in liver tissue were detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blot ***:EWM slightly reduced FBG and Fins in KKAy ***,EWM was able to downregulate serum LDL,TG,TC,and FFA and upregulate the expression of serum HDL and *** analysis revealed the following differential pathways:the peroxisome proliferator-activated receptor(PPAR)signaling pathway and the AMPK signaling ***-PCR and western blot analysis detected the associated genes and *** addition,EWM was able to upregulate the expression of AMPK and downregulate the expression of PPARγ,SREBP1c,and Fas mRNA and protein and upregulate the expression of LPL ***:EWM can alleviate lipid metabolism disorders and to some extent improve glucose metabolism disorders in KKAy *** effects may be related to regulating PPARγ/LPL and activating the