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Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks

Microsystems & Nanoengineering 2016年第1期2卷 204-212页

作者： Patrick M.Misun Jörg Rothe Yannick R.F.Schmid Andreas Hierlemann Olivier Frey ETH Zürich Department of Biosystems Science and EngineeringBio Engineering LaboratoryMattenstrasse 26Basel CH-4058Switzerland

Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out *** we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for‘body-on-a-chip’*** microfluidic platform is based on the technology of hanging-drop networks,which is designed for the formation,cultivation,and analysis of fluidically interconnected organotypic spherical three-dimensional(3D)microtissues of multiple cell *** sensor modules were designed as small glass plug-ins featuring four platinum working electrodes,a platinum counter electrode,and an Ag/AgCl reference *** were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are *** electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through *** biosensors featured high sensitivities of 322±41 nA mM^(−1) mm^(−2) for glucose and 443±37 nA mM^(−1) mm^(−2) for lactate;the corresponding limits of detection were below 10μ*** proposed technology enabled tissue-size-dependent,real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging ***,glucose consumption and lactate secretion were monitored in parallel,and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.

关键词： biosensor body on a chip glucose hanging drop lactate metabolism spheroid

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Engineering a Circular Riboregulator in Escherichia coli

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BioDesign Research 2020年第1期2020卷 9-17页

作者： William Rostain Shensi Shen Teresa Cordero Guillermo Rodrigo Alfonso Jaramillo Warwick Integrative Synthetic Biology Centre(WISB)and School of Life Sciences University of WarwickCV47AL CoventryUK Institute of Systems and Synthetic Biology CNRS-Universitéd’Évry Val-d’Essonne91000ÉvryFrance Instituto de Biología Molecular y Celular de Plantas CSIC-Universidad Politécnica de Valencia46022 ValenciaSpain Institute for Integrative Systems Biology(I2SysBio) CSIC-Universitat de València46980 PaternaSpain

RNAs of different shapes and sizes,natural or synthetic,can regulate gene expression in prokaryotes and *** RNAs have recently appeared to be more widespread than previously thought,but their role in prokaryotes remains ***,by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme,we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia *** showed that the resulting riboregulator can trans-activate gene expression by interacting with a cis-repressed messenger *** characterized the system with a fluorescent reporter and with an antibiotic resistance marker,and we modeled this novel posttranscriptional *** first reported example of a circular RNA regulating gene expression in *** adds to an increasing repertoire of RNA synthetic biology parts,and it highlights that topological molecules can play a role in the case of prokaryotic regulation.

关键词： mechanism circular sizes

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Charge Transport in Disordered Graphene-Based Low Dimensional Materials

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Nano Research 2008年第5期1卷 361-394页

作者： Alessandro Cresti Norbert Nemec Blanca Biel Gabriel Niebler François Triozon Gianaurelio Cuniberti Stephan Roche CEA LETIMINATECF38054 GrenobleFrance CEA Institute for Nanoscience and CryogenicsINAC/SPSMS/GT17 rue des Martyrs38054 Grenoble Cedex 9France Theory of Condensed Matter Group Cavendish LaboratoryUniversity of CambridgeTrinity LaneCambridge CB217NUK Institute for Materials Science TU DresdenD-01062 DresdenGermany Department of Condensed Matter Physics Faculty of Mathematics and PhysicsCharles UniversityKe Karlovu 512116 Prague 2Czech Republic

Two-dimensional graphene,carbon nanotubes,and graphene nanoribbons represent a novel class of low dimensional materials that could serve as building blocks for future carbon-based *** these systems share a similar underlying electronic structure,whose exact details depend on confi nement effects,crucial differences emerge when disorder comes into *** this review,we consider the transport properties of these materials,with particular emphasis on the case of graphene *** summarizing the electronic and transport properties of defect-free systems,we focus on the effects of a model disorder potential(Anderson-type),and illustrate how transport properties are sensitive to the underlying *** provide analytical expressions for the elastic mean free path of carbon nanotubes and graphene nanoribbons,and discuss the onset of weak and strong localization regimes,which are genuinely dependent on the transport *** also consider the effects of edge disorder and roughness for graphene nanoribbons in relation to their armchair or zigzag orientation.

关键词： Graphene charge transport carbon nanotubes

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