Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks
作者机构：ETH ZürichDepartment of Biosystems Science and EngineeringBio Engineering LaboratoryMattenstrasse 26Basel CH-4058Switzerland
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2016年第2卷第1期
页 面：204-212页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This work was financially supported by FP7 of the EU through the project‘Body on a chip’,ICT-FET-296257 the ERC Advanced Grant‘NeuroCMOS’(contract 267351)as well as by an individual Ambizione Grant 142440 from the Swiss National Science Foundation for Olivier Frey
主 题：biosensor body on a chip glucose hanging drop lactate metabolism spheroid
摘 要：Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out *** we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for‘body-on-a-chip’*** microfluidic platform is based on the technology of hanging-drop networks,which is designed for the formation,cultivation,and analysis of fluidically interconnected organotypic spherical three-dimensional(3D)microtissues of multiple cell *** sensor modules were designed as small glass plug-ins featuring four platinum working electrodes,a platinum counter electrode,and an Ag/AgCl reference *** were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are *** electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through *** biosensors featured high sensitivities of 322±41 nA mM^(−1) mm^(−2) for glucose and 443±37 nA mM^(−1) mm^(−2) for lactate;the corresponding limits of detection were below 10μ*** proposed technology enabled tissue-size-dependent,real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging ***,glucose consumption and lactate secretion were monitored in parallel,and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.
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