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Fundamental Research 2025年第1期5卷 407-418页

作者： Xiaoyan Hu Jingqi Zeng Lijuan M Xiaomeng Wang Jing Du Lu Yao Zhisheng Wu Fujian University of Traditional Chinese Medicine Fuzhou 350122China Engineering Research Center for Pharmaceutics of Chinese Materia Medica and New Drug Development Ministry of EducationBeijing 100102China Beijing University of Chinese Medicine Beijing 100102China China Beijing Tongrentang Group Co. Ltd.Beijing 100062China

This study reported an original end-to-end dataflow engineering framework for the quality transfer principle to overcome the quality challenges in real-world honey ***,650 pivotal data points of physical and chemical quality attributes from 65 batches of honey intermediates were characterized through multiple sensors,which included rheological properties,acidity,moisture,and ***,a hypersensitized TAS1R2@AuNPs/SPCE biosensor was developed to identify biological quality attributes of honey,the powerful affinities between honey intermediates and the TAS1R2 receptor were discovered(KDbiosensor and multivariable ***,the end-to-end dataflow containing physical,chemical and biological critical quality attributes was successfully established to interpret the quality transfer principle of honey manufacturing,which revealed that the front-end refining process was relatively unstable and the back-end refining process was a negligible influence on the quality of honey *** framework embraces quality management,quality transfer,and biosensor information,which will contribute to discovering the quality transfer principle in industrial innovation for intelligent manufacturing.

关键词： End-to-end dataflow Quality transfer principle Critical quality attributes biosensor Honey manufacturing

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biosensor

Chinese Journal of Biomedical Engineering(English Edition) 1993年第2期2卷 59-60页

biosensor Based on TTF@SiO_2 Nanoparticles as Electron Transfer Mediator for the Determination of Glucose

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Chemical Research in Chinese Universities 2006年第4期22卷 474-478页

作者： LI Xiao-hua XIAN Yue-zhong MIN Hong LI Chen-xin XIE Zong-hong ZHANG Fen-fen JIN Li-tong Department of Chemistry East China Normal University Shanghai 200062 P. R. China

Tetrathiafulvalene（TTF） was doped in an SiO2 network and the resulting nanocompesite was used as a mediator for the selective detection of glucose. The uniform TTF-doped silica（TIT@SiO2 ） nanoparticles were prepared by the water-in-oil（W/O） microemulsion method, and were characterized by transmission electron microscopy（TEM）. The core-shell structure TTF@ SiO2 could prevent TIT from leaching out into an aqueous solution. Combined with chitosan （CHIT）, which serves as a scaffold for glucose oxidase and nanocomposite immobilization, the GCE/TTF@ SiO2- CHIT-GOx biosensor was developed. Under optimal conditions, the biosensors exhibit a linear range of 1.0 × 10^-5 5 × 10^-3 mol/L with a detection limit down to 5.0 μmol/L（S/N = 3 ）. The excellent selectivity, sensitivity, and stability of the glucose biosensor show its potential for practical applications.

关键词： TTF@ SiO2 nanoparticle Chitosan Glucose biosensor

biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production

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Synthetic and Systems Biotechnology 2024年第2期9卷 242-249页

作者： Qiqun Peng Weiwei Bao Binan Geng Shihui Yang State Key Laboratory of Biocatalysis and Enzyme Engineering and School of Life SciencesHubei UniversityWuhan430062China

Lactate is an important monomer for the synthesis of poly-lactate(PLA),which is a substitute for the petrochemical *** achieve the goal of high lactate titer,rate,and yield for commercial production,efficient lactate production pathway is needed as well as genetic targets that affect high lactate production and *** this study,an LldR-based d-lactate biosensor with a broad dynamic range was first applied into Zymomonas mobilis to select mutant strains with strong GFP fluorescence,which could be the mutant strains with increased d-lactate ***,LldR-based d-lactate biosensor was combined with a genome-wide CRISPR interference(CRISPRi)library targeting the entire genome to generate thousands of mutants with gRNA targeting different genetic targets across the whole ***,two mutant libraries were selected containing 105 and 104 mutants with different interference sites from two rounds of fluorescence-activated cell sorting(FACS),*** genetic targets of ZMO1323 and ZMO1530 were characterized and confirmed to be associated with the increased d-lactate production,further knockout of ZMO1323 and ZMO1530 resulted in a 15%and 21%increase of d-lactate production,*** work thus not only established a high-throughput approach that combines genome-scale CRISPRi and biosensor-assisted screening to identify genetic targets associated with d-lactate production in ***,but also provided a feasible high-throughput screening approach for rapid identification of genetic targets associated with strain performance for other industrial microorganisms.

关键词： D-lactate biosensor LldR Genome-wide CRISPRi FACS Zymomonas mobilis

biosensor-based assay of exosome biomarker for early diagnosis of cancer

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Frontiers of Medicine 2022年第2期16卷 157-175页

作者： Ying Deng Zhaowei Sun Lei Wang Minghui Wang Jie Yang Genxi Li State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China

Cancer imposes a severe threat to people’s health and lives,thus pressing a huge medical and economic burden on individuals and ***,early diagnosis of cancer is indispensable in the timely prevention and effective treatment for *** has recently become an attractive cancer biomarker in noninvasive early diagnosis because of the unique physiology and pathology functions,which reflects remarkable information regarding the cancer microenvironment,and plays an important role in the occurrence and evolution of ***,biosensors have gained great attention for the detection of exosomes due to their superior properties,such as convenient operation,real-time readout,high sensitivity,and remarkable specificity,suggesting promising biomedical applications in the early diagnosis of *** this review,the latest advances of biosensors regarding the assay of exosomes were summarized,and the superiorities of exosomes as markers for the early diagnosis of cancer were ***,the recent challenges and further opportunities of developing effective biosensors for the early diagnosis of cancer were discussed.

关键词： biosensor exosome cancer diagnosis

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Real-time electrical detection of epidermal skin MoS2 biosensor for point-of-care diagnostics

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Nano Research 2017年第3期10卷 767-775页

作者： Geonwook Yoo Heekyeong Park Minjung Kim Won Geun Song Seokhwan Jeong Min Hyung Kim Hyungbeen Lee Sang Woo Lee Young KiHong Min Goo Lee Sungho Lee Sunkook Kim School of Electronic Engineering Soongsil University Seou106978 Republic of Korea Multi-Functional Nano/Bio Electronics Lab. Kyung Hee University Gyeonggi 17104 Republic of Korea Department of Biomedical Engineering Yonsei University Wonju 26493 Republic of Korea Korea Electronics Technology Institute Gyeonggi 13488 Republic of Korea

Various approaches have been proposed for point-of-care diagnostics, and in particular, optical detection is preferred because it is relatively simple and fast. At the same time, field-effect transistor （FET）-based biosensors have attracted great attention because they can provide highly sensitive and label-free detection. In this work we present highly sensitive, epidermal skin-type point-of-care devices with system-level integration of flexible MOS2 FET biosensors, read-out circuits, and light-emitting diode （LEDs） that enable real-time detection of prostate cancer antigens （PSA）. Regardless of the physical forms or mechanical stress conditions, our proposed high-performance MoS2 biosensors can detect a PSA concentration of 1 ***-1 without specific surface treatment for anti-PSA immobilization on the MoS2 surface on which we characterize and confirm physisorption of anti-PSA using Kelvin probe force microscopy （KPFM） and tapping-mode atomic force microscopy （tm-AFM）. Furthermore, current modulation induced by the binding process was stably maintained for longer than 2-3 min. The results indicate that flexible MoS2-based FET biosensors have great potential for point-of-care diagnostics for prostate cancer as well as other biomarkers.

关键词： epidermal skin biomarker MoS2 biosensor real-time point-of-care diagnostics

Highly sensitive,scalable,and rapid SARS-CoV-2 biosensor based on In_(2)O_(3)nanoribbon transistors and phosphatase

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Nano Research 2022年第6期15卷 5510-5516页

作者： Mingrui Chen Dingzhou Cui Zhiyuan Zhao Di Kang Zhen Li Shahad Albawardi Shahla Alsageer Faisal Alamri Abrar Alhazmi Moh.R.Amer Chongwu Zhou Mork Family Department of Chemical Engineering and Materials Science University of Southern CaliforniaLos AngelesCalifornia 90089USA Ming Hsieh Department of Electrical Engineering University of Southern CaliforniaLos AngelesCalifornia 90089USA Department of Physics and Astronomy University of Southern CaliforniaLos AngelesCalifornia 90089USA Center of Excellence for Green Nanotechnologies King Abdulaziz City for Science and TechnologyRiyadhSaudi Arabia Department of Electrical Engineering 420 Westwood Plaza5412 Boelter HallUniversity of CaliforniaLos AngelesLos AngelesCalifornia 90095USA eDNA Biotech PasadenaCalifornia 91107USA

Developing convenient and accurate SARS-CoV-2 antigen test and serology test is crucial in curbing the global COVID-19 *** this work,we report an improved indium oxide(In2O3)nanoribbon field-effect transistor(FET)biosensor platform detecting both SARS-CoV-2 antigen and *** FET biosensors,which were fabricated using a scalable and cost-efficient lithography-free process utilizing shadow masks,consist of an In_(2)O_(3)channel and a newly developed stable enzyme *** the biosensing process,the phosphatase enzymatic reaction generated pH change of the solution,which was then detected and converted to electrical signal by our In_(2)O_(3)*** biosensors applied phosphatase as enzyme reporter,which has a much better stability than the widely used urease in FET based *** proof-of-principle studies,we demonstrate the detection of SARS-CoV-2 spike protein in both phosphate-buffered saline(PBS)buffer and universal transport medium(UTM)(limit of detection[LoD]:100 fg/mL).Following the SARS-CoV-2 antigen tests,we developed and characterized additional sensors aimed at SARS-CoV-2 IgG antibodies,which is important to trace past infection and *** spike protein IgG antibody tests exhibit excellent detection limits in both PBS and human whole blood((LoD):1 pg/mL).Our biosensors display similar detection performance in different mediums,demonstrating that our biosensor approach is not limited by Debye screening from salts and can selectively detect biomarkers in physiological *** newly selected enzyme for our platform performs much better performance and longer shelf life which will lead our biosensor platform to be capable for real clinical diagnosis usage.

关键词： biosensor indium oxide transistor phosphatase SARS-CoV-2 spike protein SARS-CoV-2 spike IgG antibody

A screen-printed, amperometric biosensor for the determination of organophosphorus pesticides in water samples

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Journal of Environmental Sciences 2012年第5期24卷 956-962页

作者： Raju Sekar Department of Biological Sciences Xi'an Jiaotong-Liverpool University

An amperometric biosensor based on screen-printed electrodes （SPEs） was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase （AChE） deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride （ATChCl） concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides （dichlorvos, monocrotophs and parathion） were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.

关键词： organophosphorus pesticides acetylcholinesterase enzyme immobilization screen-printed electrodes biosensor

Surface cleaned Cu_(x)Pd_(y)alloy:Synthesis and their superior performances in hydrogen evolution reaction and biosensor

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Nano Research 2023年第5期16卷 7941-7949页

作者： Xinmei Liu Xue Li Wenlong Yang Gang Liu Yu Wang Yuhang Zuo Harbin University of Science and Technology Harbin 150001China Jiangsu Zhongsheng Gaoke Environment Co. Ltd.Wuxi 214244China

High cost and restricted activity of electrocatalysis are the major challenges for hydrogen generation and *** this work,we provided a one-pot synthesis of Cu_(x)Pd_(y)alloy nanoparticles(NPs)with controllable atomic ratio and“clean surface”.Benefiting from the preferable d-band structure,the Cu_(62)Pd_(38)NPs exhibited a lower overpotentials in the hydrogen evolution reaction(HER)over the full pH *** the acidic media,Cu_(62)Pd_(38)NPs achieved a low overpotential of 28.12 mV for HER,which was 25.73%of Pd *** the neutral solution,the overpotential by Cu_(62)Pd_(38)NPs is only 41.71%for that by uncleaned CuPd *** alkaline media,the overpotential by Cu_(62)Pd_(38)NPs was declined from 38.01 to 20.20 mV after 720 min yielding hydrogen,which was only 53.14%for the initial *** applied in biosensor,the synergistic effect of Cu and Pd accelerated the kinetics of electrocatalytic process,resulting in an enhanced *** glucose sensor constructed by Cu_(67)Pd_(33)exhibited a wider detection range up to 100.0 *** the sensitivity is 379.4μA/(mM·cm^(2)),which is ca.4.63 and 14.09 folds for that by pure Cu NPs and Pd NPs,*** optimal atomic percent would be conducive to optimize electrocatalytic activity of Cu_(x)Pd_(y)*** volcano plots for Cu_(x)Pd_(y)would open up a new avenue for designing electrocatalysis with rationalized cost and optimized performance.

关键词： alloy biosensor hydrogen evolution reaction overpotential detection range

A direct electrochemical biosensor for rapid glucose detection based on nitrogen-doped carbon nanocages

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Rare Metals 2024年第5期43卷 2184-2192页

作者： Lan-Lan Li Yu Zhao Li-Jia Pan Jian-Bin Xu Yi Shi College of Mechanical and Electrical Engineering Henan Agricultural UniversityZhengzhou 450002China School of Electronic Science and Engineering Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing210093China Department of Electronic Engineering The Chinese University of HongKongHongkong 999077China

Given the increasing number of diabetic patients,rapid and accurate detection of glucose in body fluids is *** study developed a direct electrochemical biosensor for glucose based on nitrogen-doped carbon nanocages(NCNCs).NCNCs possess a large specific surface area of 1395 m^(2)·g^(-1),a high N atomic content of 9.37%and good biocompatibility,which is favorable for enzyme loading and electron *** surface average concentration of electroactive glucose oxidase on NCNCs was 2.82×10^(-10)mol·cm^(-2).The NCNC-based direct electrochemical biosensor exhibited a high sensitivity of 13.7μA·(mmol·L^(-1))^(-1)·cm^(-2),rapid response time of 5 s and an impressive electron-transferrate constant(ks)of 1.87 s^(-1).Furthermore,we investigated an NCNC-based direct electron transfer(DET)biosensor for sweat glucose detection,which demonstrated tremendous promise for non-invasive wearable diabetes diagnosis.

关键词： Direct electron transfer(DET) Nitrogen-doped carbon nanocages(NCNCs) Glucose oxidase biosensor