A screen-printed, amperometric biosensor for the determination of organophosphorus pesticides in water samples

作     者：Raju Sekar 

作者机构：Department of Biological Sciences Xi'an Jiaotong-Liverpool University 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2012年第24卷第5期

页      面：956-962页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 0804[工学-仪器科学与技术] 

基　　金：supported by the National Natural Science Foundation of China (No. 51008026  40873076 and 41011130204) 

主　　题：organophosphorus pesticides acetylcholinesterase enzyme immobilization screen-printed electrodes biosensor 

摘      要：An amperometric biosensor based on screen-printed electrodes （SPEs） was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase （AChE） deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride （ATChCl） concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides （dichlorvos, monocrotophs and parathion） were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.