Biosensor Based on TTF@SiO_2 Nanoparticles as Electron Transfer Mediator for the Determination of Glucose

作     者：LI Xiao-hua XIAN Yue-zhong MIN Hong LI Chen-xin XIE Zong-hong ZHANG Fen-fen JIN Li-tong 

作者机构：Department of Chemistry East China Normal University Shanghai 200062 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2006年第22卷第4期

页      面：474-478页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：Supported by the National Natural Science Foundation of China(No. 20305007) and Doctoral Foundation of China Ministry ofEducation(No. 20030269014) 

主　　题：TTF@ SiO2 nanoparticle Chitosan Glucose Biosensor 

摘      要：Tetrathiafulvalene（TTF） was doped in an SiO2 network and the resulting nanocompesite was used as a mediator for the selective detection of glucose. The uniform TTF-doped silica（TIT@SiO2 ） nanoparticles were prepared by the water-in-oil（W/O） microemulsion method, and were characterized by transmission electron microscopy（TEM）. The core-shell structure TTF@ SiO2 could prevent TIT from leaching out into an aqueous solution. Combined with chitosan （CHIT）, which serves as a scaffold for glucose oxidase and nanocomposite immobilization, the GCE/TTF@ SiO2- CHIT-GOx biosensor was developed. Under optimal conditions, the biosensors exhibit a linear range of 1.0 × 10^-5 5 × 10^-3 mol/L with a detection limit down to 5.0 μmol/L（S/N = 3 ）. The excellent selectivity, sensitivity, and stability of the glucose biosensor show its potential for practical applications.