Engineering a Circular Riboregulator in Escherichia coli

作     者：William Rostain Shensi Shen Teresa Cordero Guillermo Rodrigo Alfonso Jaramillo 

作者机构：Warwick Integrative Synthetic Biology Centre(WISB)and School of Life SciencesUniversity of WarwickCV47AL CoventryUK Institute of Systems and Synthetic BiologyCNRS-Universitéd’Évry Val-d’Essonne91000ÉvryFrance Instituto de Biología Molecular y Celular de PlantasCSIC-Universidad Politécnica de Valencia46022 ValenciaSpain Institute for Integrative Systems Biology(I2SysBio)CSIC-Universitat de València46980 PaternaSpain 

出 版 物：《BioDesign Research》 (生物设计研究（英文）)

年 卷 期：2020年第2020卷第1期

页      面：9-17页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by grants FP7-ICT-FET 610730(EVOPROG)and 613745(PROMYS),BBSRC BB/P020615/1(EVO-ENGINE),and EPSRC-BBSRC BB/M017982/1(WISB center) the startup allocation from the School of Life Sciences to A.J.and by the grant BFU2015-66894-P from MINECO and ERDF to G.R.W.R.is supported by a DGA graduate fellowship G.R.is partly supported by the AXA Research Fund 

主　　题：mechanism circular sizes 

摘      要：RNAs of different shapes and sizes,natural or synthetic,can regulate gene expression in prokaryotes and *** RNAs have recently appeared to be more widespread than previously thought,but their role in prokaryotes remains ***,by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme,we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia *** showed that the resulting riboregulator can trans-activate gene expression by interacting with a cis-repressed messenger *** characterized the system with a fluorescent reporter and with an antibiotic resistance marker,and we modeled this novel posttranscriptional *** first reported example of a circular RNA regulating gene expression in *** adds to an increasing repertoire of RNA synthetic biology parts,and it highlights that topological molecules can play a role in the case of prokaryotic regulation.