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OsbZIP72 Is Involved in Transcriptional Gene-Regulation Pathway of Abscisic Acid Signal Transduction by Activating Rice High-Affinity Potassium Transporter OsHKT1;1

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Rice science 2021年第3期28卷 257-267页

作者： WANG Baoxiang LIU Yan WANG Yifeng LI Jingfang SUN Zhiguang CHI Ming xing yungao XU Bo YANG Bo LI Jian LIU Jinbo CHEN Tingmu FANG Zhaowei LU Baiguan XU Dayong Babatunde Kazeem BELLO Lianyungang Institute of Agricultural Sciences Collaborative Innovation Center for Modern Crop ProductionLianyungang 222006China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China

We created CRISPR-Cas9 knock-out and overexpressing OsbZIP72 transgenic rice plants to gain a better understanding of the role and molecular mechanism of OsbZIP72 gene in stress tolerance,which has remained largely ***72 was expressed and integrated into rice transgenic plant genomes,and the OsbZIP72 transcript in overexpression lines was elicited by salinity,abscisic acid(ABA)and drought ***72 overexpressing plants showed higher tolerance to drought and salinity stresses,while knock-out transgenic lines showed higher sensitivity to these *** differentially expressed genes(DEGs)from RNA-sequencing data encompassed several abiotic stress genes,and the functional classification of these DEGs demonstrated the robust transcriptome diversity in *** one-hybrid,along with luciferase assay,indicated that OsbZIP72 acted as a transcriptional ***,electrophoresis mobility assay revealed that OsbZIP72 bound directly to the ABAresponsive element in the OsHKT1;1 promoter region and activated its ***,our findings revealed that OsbZIP72 can act as a transcriptional modulator with the ability to induce the expression of OsHKT1;1 in response to environmental stress through an ABA-dependent regulatory pathway,indicating that OsbZIP72 can play a crucial role in the ABA-mediated salt and drought tolerance pathway in rice.

关键词： abscisic acid basic leucine zipper drought stress high-affinity potassium transporter rice salinity stress transgenic plant

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Genome-wide association study and linkage analysis of quantative resistance to rice black-streaked dwarf virus disease in rice

Genome-wide association study and linkage analysis of quanta...

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2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Baoxiang Wang Bo Xu Yan Liu yungao xing Bo Yang Jian Li Ming Chi Jinbo Liu Tingmu Chen Dayong Xu Lianyungang Academy of Agricultural Science

Rice black-streaked dwarf virus disease（RBSDVD） transmitted by small brown planthopper（SBPH, Laodelphax striatellus） is one of the most destructive viral disease in rice（Oryza sativa L.） production. It is urgent to explore resistance resources and genes to breed resistant cultivars for controlling this disease. In present study, 1071 sequenced diverse rice accessions collected from 72 countries or regions were evaluated for resistance to RBSDVD. Totally, 3 significant associated loci were detected by Genome-wide association study（GWAS）, a locus on chromosome 6 was further verified by a bi-parental genetic population. In the significant association interval with RBSDVD resistance, 20 adjacent candidate genes were predicted. The study will provide a powerful resource for elucidating the mechanism underlying RBSDVD resistance and breeding resistance rice cultivars by molecular assisted selection（MAS）.

关键词： Rice black-streaked dwarf virus(RBSDV) Rice(Oryza sativa L.) Genome-wide association study(GWAS)

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Genetic variation of drought resistance of rice genotypes released in China

Genetic variation of drought resistance of rice genotypes re...

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2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Baoxiang Wang Bo Xu Yan Liu Xubing Chen Wenfang Zhi yungao xing Bo Yang Jian Li Ming Chi Jinbo Liu Tingmu Chen Dayong Xu Guanglong Zhu Lianyungang Academy of Agricultural Science Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou University

Drought is the most serious factor limiting rice production worldwide and it is becoming intensively with climate change in recent years. Therefore, to screen and breed drought tolerant rice genotype is essential to maintain yield gain and ensure food security under adversity environment. However, thus far, little attention has given to the temporal changes of drought resistance concomitant with the year of release in rice genetic breeding. Therefore, the objective of this study was to explore the variation of drought resistance of rice genotypes with year of release in field experiment. Sixteen rice(Oryza sativa L.) genotypes bred or widely cultivated from 1934 to 2007 were grown in paddy field(with normal water condition, CK) and upland field(drought stress, DT). Grain yield and daily grain yield were significantly increased with the year of release. The genetic improvements in the grain yield partially resulted from the significant increase in panicle, spikelets per panicle and filling percentage. In addition, various growth related characteristics like biomass, harvest index(HI), relative growth rate(RGR), grain density and growth duration increased with increase of genetic refinement of the genotypes through the years and they contributed to improvement of final rice yields. However, grain yield, yield components and these growth related traits sharply declined under drought stress. Furthermore, the drought resistance coefficients of K

关键词： Grain yield drought resistance drought resistance coefficient Genetic improvement Rice

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Genomic survey, characterization and expression profile analysis of the 3-phosphoglycerate dehydrogenase family in rice（Oryza sativa L.）

Genomic survey, characterization and expression profile anal...

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江苏省遗传学会2019年学术研讨会

作者： Baoxiang Wang Tao Guo Haifeng Wang Jingfang Li Zhiguang Sun Yan Liu yungao xing Bo Xu Bo Yang Jian Li Ming Chi Jinbo Liu Tingmu Chen Guanglong Zhu Dayong Xu Lianyungang Academy of Agricultural Science Shandong Rice Research Institute/Shandong Rice Engineering Technology Research Center Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou University

The synthesis of serine in rice is intangible, and little kinetic and structural information is available for most enzymes in the pathway. In this study, we carried out a comprehensive characteristic and phylogenetic analysis of three 3-phosphoglycerate dehydrogenase（PGDH） members in rice, as well as an analysis of their whole-life expression patterns. All of the OsPGDHs have three highly conserved motifs. The three genes were detected in all tissues and development stages in Nipponbare by qRT-PCR. Under abiotic stresses, such as salt（150 mM NaCl）, drought（20% PEG 4000 simulation）, oxidation（10 mM H2O2） and low temperature（4±1 ℃）, the expressions of all OsPGDHs are significantly different. These investigations improve our understanding of the importance of the OsPGDH family and provide useful information about the phosphorylated serine biosynthesis pathway in rice.

关键词： 3-phosphoglycerate dehydrogenase rice abiotic stresses Serine

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Loss Aware Feature Attention Mechanism for Class and Feature Imbalance Issue

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Computers, Materials & Continua 2025年第1期82卷 751-775页

作者： Yuewei Wu Ruiling Fu Tongtong xing Fulian Yin College of Information and Communication Engineering Communication University of ChinaBeijing100024China State Key Laboratory of Media Convergence and Communication Communication University of ChinaBeijing100024China

In the Internet era,recommendation systems play a crucial role in helping users find relevant information from large *** imbalance is known to severely affect data quality,and therefore reduce the performance of recommendation *** to the imbalance,machine learning algorithms tend to classify inputs into the positive(majority)class every time to achieve high prediction *** can be categorized such as by features and classes,but most studies consider only class *** this paper,we propose a recommendation system that can integrate multiple networks to adapt to a large number of imbalanced features and can deal with highly skewed and imbalanced datasets through a loss *** propose a loss aware feature attention mechanism(LAFAM)to solve the issue of feature *** network incorporates an attention mechanism and uses multiple sub-networks to classify and learn *** better results,the network can learn the weights of sub-networks and assign higher weights to important *** propose suppression loss to address class imbalance,which favors negative loss by penalizing positive loss,and pays more attention to sample points near the decision *** on two large-scale datasets verify that the performance of the proposed system is greatly improved compared to baseline methods.

关键词： Imbalanced data deep learning e-commerce recommendation loss function big data analysis

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粳糯稻抗稻瘟病基因检测与品种抗性鉴定

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核农学报 2023年第11期37卷 2134-2141页

作者：邢运高刘艳李健迟铭杨波李景芳王宝祥徐大勇江苏省连云港市农业科学院/江苏省现代作物生产协同创新中心江苏连云港222006

为了探明糯稻的稻瘟病抗性水平和抗稻瘟病基因分布,评价抗稻瘟病基因的抗性效应,利用自然诱发鉴定与人工接种鉴定相结合的方法鉴定65个糯稻品种(品系)的稻瘟病抗性,利用竞争性等位基因特异性PCR(KASP)标记技术检测Pi2、Pi9、Pigm、Pi1、... 详细信息

为了探明糯稻的稻瘟病抗性水平和抗稻瘟病基因分布,评价抗稻瘟病基因的抗性效应,利用自然诱发鉴定与人工接种鉴定相结合的方法鉴定65个糯稻品种(品系)的稻瘟病抗性,利用竞争性等位基因特异性PCR(KASP)标记技术检测Pi2、Pi9、Pigm、Pi1、Pikh、Pik、Pi5、Pita等8个基因。2021年自然诱发鉴定、2021—2022年人工接种鉴定中抗以上品种(品系)的比例分别是41.6%、24.6%和6.2%,说明糯稻品种(品系)的稻瘟病抗性年际间波动大、整体抗病性一般。除了Pigm外,其他7个抗病基因都有分布,分布频率差异较大,Pita基因在供试材料中分布频率最高,达到了67.7%,其次是Pikh、Pi9、Pi2、Pi5、Pik、Pi1,分布频率为47.7%、38.5%、27.7%、13.8%、4.6%、1.5%。供试糯稻品种(品系)携带0~4个抗病基因不等,仅有连糯19105携带4个抗性基因。国审、江苏审定的品种携带Pita的比例最高,安徽审定品种携带Pi9的比例最高,山东审定品种携带Pi5的比例最高,但不携带Pi2基因,Pik基因仅存在于国审及江苏审的品种中。携带3个抗性基因的供试品种(品系)抗性最好,其中“Pi2+Pita+Pik”、“Pi9+Pita+Pik”连续两年的自然诱发鉴定和人工接种鉴定抗病品种(品系)的比例均达到100%,年际间抗病稳定,可作为改良粳糯稻瘟病抗性主要的基因组合。本研究结果对粳糯稻的抗稻瘟病改良提供了理论依据。

关键词：糯稻稻瘟病 KASP 抗性分析

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Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

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Nano-Micro Letters 2025年第1期17卷 281-292页

作者： xing Wang Wei Pi Sheng Hu Haifeng Bao Na Yao Wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

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Optimal investment, retirement, and life insurance with consumption ratcheting and time-inhomogeneous utility

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Science China Mathematics 2025年

作者： Jie xing Jingtang Ma School of Big Data Application and Economics Guizhou University of Finance and Economics School of Mathematics Southwestern University of Finance and Economics

In this paper, we study a portfolio selection problem of an investor with a retirement option, who has possibility to buy life insurance and does not tolerate any decline in consumption. The agent's optimization problem can be viewed as a mixed singular control and optimal stopping problem with time-inhomogeneous utility functions. The closed-form optimal solution is not available in general. We use the dual control method to convert the original problem into two classes of optimal stopping problems in finite and infinite *** show that the optimal consumption strategy and the best retirement time depend on the free-boundary functions which satisfy Fredholm and Volterra integral equations. We derive the closed-form formulas for these two free boundaries for some special cases and develop numerical methods to solve the integral equations of the free boundaries for general cases.

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水稻赖氨酸含量测定方法改进及其最优动态分类研究

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中国稻米 2021年第2期27卷 51-54页

作者：陈庭木李景芳邢运高徐波王宝祥徐大勇江苏省连云港市农业科学院/江苏省现代作物生产协同创新中心江苏连云港222006

赖氨酸是水稻营养成分中第一限制性氨基酸。为优化赖氨酸的测定方法,设置了本试验,通过超声振荡器取代普通振荡器,进行300W超声功率下酰化用时及染料结合反应用时的研究。结果表明,最佳酰化用时为15min,染料结合用时为90 min。与国标法... 详细信息

赖氨酸是水稻营养成分中第一限制性氨基酸。为优化赖氨酸的测定方法,设置了本试验,通过超声振荡器取代普通振荡器,进行300W超声功率下酰化用时及染料结合反应用时的研究。结果表明,最佳酰化用时为15min,染料结合用时为90 min。与国标法相比,优化后的方法酰化用时变长、染料结合用时变短,测定总用时明显缩短,但测定结果和试验精度更高。可见,采用超声波振荡代替传统振荡器振荡能改进赖氨酸的测定方法。另外,采用最优动态聚类法对赖氨酸含量进行分类,能防止人为分类的不确定性,做到分类方案的最优化。

关键词：赖氨酸含量测定方法动态聚类轮回选择算法

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Stiffness-tunable biomaterials provide a good extracellular matrix environment for axon growth and regeneration

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Neural Regeneration Research 2025年第5期20卷 1364-1376页

作者： Ronglin Han Lanxin Luo Caiyan Wei Yaru Qiao Jiming Xie Xianchao Pan Juan xing Department of Pathophysiology School of Basic Medical SciencesSouthwest Medical UniversityLuzhouSichuan ProvinceChina Department of Medicinal Chemistry School of PharmacySouthwest Medical UniversityLuzhouSichuan ProvinceChina

Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.

关键词： alginate axon growth biomaterials extracellular matrix neural repair neurons neuroregeneration polyacrylamide polydimethylsiloxane stiffness

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