OsbZIP72 Is Involved in Transcriptional Gene-Regulation Pathway of Abscisic Acid Signal Transduction by Activating Rice High-Affinity Potassium Transporter OsHKT1;1

作     者：WANG Baoxiang LIU Yan WANG Yifeng LI Jingfang SUN Zhiguang CHI Ming XING Yungao XU Bo YANG Bo LI Jian LIU Jinbo CHEN Tingmu FANG Zhaowei LU Baiguan XU Dayong Babatunde Kazeem BELLO WANG Baoxiang;LIU Yan;WANG Yifeng;LI Jingfang;SUN Zhiguang;CHI Ming;XING Yungao;XU Bo;YANG Bo;LI Jian;LIU Jinbo;CHEN Tingmu;FANG Zhaowei;LU Baiguan;XU Dayong;Babatunde Kazeem BELLO

作者机构：Lianyungang Institute of Agricultural SciencesCollaborative Innovation Center for Modern Crop ProductionLianyungang 222006China State Key Laboratory of Rice BiologyChina National Rice Research InstituteHangzhou 311400China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2021年第28卷第3期

页      面：257-267页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the earmarked funds for China Agriculture Research System(Grant No.CARS-01-61) National Science and Technology Support Program of China(Grant No.2015BAD01B01) Science and Technology Support Program of Jiangsu Province,China(Grant Nos.BE2016370-3 and BE2017323) the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20161299) the Financial Grant Support Program of Lianyungang City,Jiangsu Province,China(Grant Nos.QNJJ1704 and QNJJ1912) National Natural Science Foundation of China(Grant No.31701395) 

主　　题：abscisic acid basic leucine zipper drought stress high-affinity potassium transporter rice salinity stress transgenic plant 

摘      要：We created CRISPR-Cas9 knock-out and overexpressing OsbZIP72 transgenic rice plants to gain a better understanding of the role and molecular mechanism of OsbZIP72 gene in stress tolerance,which has remained largely ***72 was expressed and integrated into rice transgenic plant genomes,and the OsbZIP72 transcript in overexpression lines was elicited by salinity,abscisic acid(ABA)and drought ***72 overexpressing plants showed higher tolerance to drought and salinity stresses,while knock-out transgenic lines showed higher sensitivity to these *** differentially expressed genes(DEGs)from RNA-sequencing data encompassed several abiotic stress genes,and the functional classification of these DEGs demonstrated the robust transcriptome diversity in *** one-hybrid,along with luciferase assay,indicated that OsbZIP72 acted as a transcriptional ***,electrophoresis mobility assay revealed that OsbZIP72 bound directly to the ABAresponsive element in the OsHKT1;1 promoter region and activated its ***,our findings revealed that OsbZIP72 can act as a transcriptional modulator with the ability to induce the expression of OsHKT1;1 in response to environmental stress through an ABA-dependent regulatory pathway,indicating that OsbZIP72 can play a crucial role in the ABA-mediated salt and drought tolerance pathway in rice.