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Metal-free intramolecular hydroarylation of alkynes

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Organic Chemistry Frontiers 2022年第5期9卷 1300-1307页

作者： Chaofeng Zhang Songkui Lv Yanru wang Jingyi Zhang xiao-na wang Junbiao Chang Collaborative Innovation Center of New Drug Research and Safety Evaluation Henan ProvinceKey Laboratory of Advanced Drug Preparation TechnologiesMinistry of EducationSchool of Pharmaceutical SciencesZhengzhou UniversityZhengzhou 450001P.R.China

An efficient metal-free intramolecular hydroarylation reaction of alkynes is described here.A series of aryl and N-group attached alkynes generated the intramolecular hydroarylation products in high yields.

关键词： intramolecular alkynes free

MdbHLH162 connects the gibberellin and jasmonic acid signals to regulate anthocyanin biosynthesis in apple

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Journal of Integrative Plant Biology 2024年第2期66卷 265-284页

作者： Jian-Ping An Rui-Rui Xu xiao-na wang xiao-Wei Zhang Chun-Xiang You Yuepeng Han Apple technology innovation center of Shandong Province College of Horticulture Science and EngineeringShandong Agricultural UniversityTai-An 271018China CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenHubei Hongshan LaboratoryThe Innovative Academy of Seed Design of Chinese Academy of SciencesWuhan 430074China College of Biology and Oceanography Weifang UniversityWeifang 261061China

Anthocyanins are secondary metabolites induced by environmental stimuli and developmental *** positive regulators of anthocyanin biosynthesis have been reported,whereas the anthocyanin repressors have been *** the signal transduction pathways of gibberellin(GA)and jasmonic acid(JA)and their regulation of anthocyanin biosynthesis have been investigated,the cross-talk between GA and JA and the antagonistic mechanism of regulating anthocyanin biosynthesis remain to be *** this study,we identified the anthocyanin repressor MdbHLH162 in apple and revealed its molecular mechanism of regulating anthocyanin biosynthesis by integrating the GA and JA ***162 exerted passive repression by interacting with MdbHLH3 and MdbHLH33,which are two recognized positive regulators of anthocyanin ***162 negatively regulated anthocyanin biosynthesis by disrupting the formation of the anthocyanin-activated MdMYB1-MdbHLH3/33complexes and weakening transcriptional activation of the anthocyanin biosynthetic genes MdDFR and MdUF3GT by MdbHLH3 and *** GA repressor MdRGL2a antagonized MdbHLH162-mediated inhibition of anthocyanins by sequestering MdbHLH162 from the MdbHLH162-MdbHLH3/33 *** JA repressors MdJAZ1 and MdJAZ2 interfered with the antagonistic regulation of MdbHLH162 by MdRGL2a by titrating the formation of the MdRGL2a-MdbHLH162 *** findings reveal that MdbHLH162 integrates the GA and JA signals to negatively regulate anthocyanin *** study provides new information for discovering more anthocyanin biosynthesis repressors and explores the cross-talk between hormone signals.

关键词： anthocyanin biosynthesis bHLH transcription factor gibberellin jasmonic acid regulatory network transcriptional regulation

B cell receptor signaling pathway involved in benign lymphoepithelial lesions of the lacrimal gland

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 665-669页

作者： xiao-na wang Xin Ge Jing Li xiao Liu Jian-Min Ma Beijing Tongren Eye Center Beijing Institute of OphthalmologyBeijing Tongren HospitalBeijing Ophthalmology&Vision Science Key LabCapital Medical UniversityBeijing 100730China

AIM：To detect the expression of B cell receptor signaling pathway（BCRSP） in lacrimal gland benign lymphoepithelial lesions（LGBLEL）.METHODS：Gene microarray was used to compare whole-genome expression in lacrimal gland tissues from LGBLEL patients to tissues from orbital cavernous hemangioma（control tissues）. Expression of BCRSP was confirmed by polymerase chain reaction（PCR） and immunohistochemistry. RESULTS：The expression of 22 genes of the BCRSP increased significantly in LGBLEL patients. PCR analysis showed that CD22, CR2, and BTK were all highly expressed in LGBLEL tissues. Immunohistochemical analysis showed that CR2 protein was present in LGBLEL, but CD22 and BTK proteins were negative. CR2, CD22, and BTK were not observed in the orbital cavernous hemangiomas with either PCR or immunohistochemistry. CONCLUSION：BCRSP might be involved in the pathogenesis of LGBLEL.

关键词： lacrimal gland benign lymphoepitheliallesion B cell receptor signaling pathway pathogenesis

Protocol for the FACE study:frailty and comorbidity in elderly patients—a multicenter,Chinese observational cohort study

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Journal of Geriatric Cardiology 2023年第1期20卷 83-90页

作者： Wen ZHENG Xin HUANG Min SUO xiao wang Xue-Dong ZHAO Wei GONG Yan YAN xiao-na wang Li SHENG Shao-Ping NIE Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijingChina Department of Cardiology Beijing Shijitan HospitalCapital Medical UniversityBeijingChina Department of Geriatric Cardiology National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijingChina

The present protocol describes an observational cohort study that was designed to propose a therapeutic scheme and formulate an individualized treatment strategy for frail elderly patients diagnosed with multiple diseases in a Chinese,multicenter *** a 3-year period,we will recruit 30,000 patients from 10 hospitals and collect baseline data including patient demographic information,comorbidity characteristic,FRAIL scale,age-adjusted Charlson comorbidity index(aCCI),relevant blood tests,the results of imaging examination,prescription of drugs,length of hospital stay,number of overall re-hospitalizations and *** patients(≥65 years old)with multimorbidity and receiving hospital care are eligible for this *** collection is being performed at baseline and 3,6,9 and 12 months after *** primary analysis was all-cause death,readmission rate and clinical events(including emergency visits,stroke,heart failure,myocardial infarction,tumor,acute chronic obstructive pulmonary disease,etc).The study is approved by the national Key R&D Program of China(2020YFC2004800).Data will be disseminated in manuscripts submitted to medical journals and in abstracts submitted to international geriatric *** Trial Registration:[***],identifier[ChiCTR2200056070].

关键词： patients morbidity prescription

The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

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Horticulture Research 2017年第1期4卷 246-254页

作者： Jian-Ping An Feng-Jia Qu Ji-Fang Yao xiao-na wang Chun-Xiang You xiao-Fei wang Yu-Jin Hao national Key Laboratory of Crop Biology MOA Key Laboratory of Horticultural Crop Biology and Germplasm InnovationCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’An271018ShandongChina College of Life Science Shandong Agricultural UniversityTai’An271018ShandongChina

The basic leucine zipper(bZIP)transcription factor HY5 plays a multifaceted role in plant growth and *** the apple MdHY5 gene was cloned based on its homology with Arabidopsis *** analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid *** mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs,***,we obtained transgenic apple calli that overexpressed the MdHY5 gene,and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis *** addition,the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were *** association with increased nitrate reductase activities and nitrate contents,the results indicated that MdHY5 might be an important regulator in nutrient *** together,these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.

关键词： HY5 nitrate assimilation

Effects of extended lymphadenectomy and postoperative chemotherapy on node-negative gastric cancer

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World Journal of Gastroenterology 2013年第33期19卷 5551-5556页

作者： Qiang Xue xiao-na wang Jing-Yu Deng Ru-Peng Zhang Han Liang Department of Gastric Cancer Surgery Tianjin Medical University Cancer Hospital and City Key Laboratory of Cancer Prevention and Therapy

AIM:To investigate the effects of extended lymphadenectomy and postoperative chemotherapy on gastric cancer without lymph node ***:Clinical data of 311 node-negative gastric cancer patients who underwent potentially curative gastrectomy with more than 15 lymph nodes resected,from January 2002 to December 2006,were analyzed *** with pT4 stage or distant metastasis were *** analyzed the relationship between the D2 lymphadenectomy and the 5-year survival rate among different subgroups stratified by clinical features,such as age,tumor size,tumor location and depth of *** the same time,the relationship between postoperative chemotherapy and the5-year survival rate among different subgroups were also ***:The overall 5-year survival rate of the entire cohort was 63.7%.The 5-year survival rate was poor in those patients who were:(1)more than 65 years old;(2)with tumor size larger than 4 cm;(3)with tumor located in the upper portion of the stomach;and(4)with pT3 *** survival rate was improved significantly by extended lymphadenectomy only in patients with pT3 tumor(P=0.019),but not in other ***,there was no significant difference in survival rate between patients with and without postoperative chemotherapy among all of the subgroups(P>0.05).CONCLUSION:For gastric cancer patients without lymph node metastasis,extended lymphadenectomy could improve the survival rate of those who have pT3-stage ***,there was no evidence of a survival benefit from postoperative chemotherapy alone.

关键词： Gastric cancer Lymph node negative metastasis Extended lymphadenectomy D2 lymphadenectomy Chemotherapy

Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit

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natural Products and Bioprospecting 2023年第1期13卷 627-638页

作者： xiao-na wang Zhao-Jie wang Yun Zhao Huan wang Mei-Ling Xiang Yang-Yang Liu Li-Xing Zhao xiao-Dong Luo Key Laboratory of Medicinal Chemistry for natural Resource Ministry of Education and Yunnan ProvinceYunnan Characteristic Plant Extraction LaboratorySchool of Chemical Science and TechnologyYunnan UniversityKunming 650500People’s Republic of China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of Sciences KunmingKunming 650201People’s Republic of China

Postharvest pathogens can affect a wide range of fresh fruit and vegetables,including grapes,resulting in significant profit *** alkaloids of Mahonia fortunei,a Chinese herbal medicine,have been used to treat infectious microbes,which might be effective against postharvest *** phytochemical and bioactive investigation of this plant led to the isolation of 18 alkaloids,of which 9 compounds inhibited the growth of Botrytis cinerea and 4 compounds against Penicillium *** antifungal alkaloids could change the mycelium morphology,the total lipid content,and leak the cell contents of ***,the two most potent antifungal alkaloids,berber-ine(13)completely inhibited effect on gray mold of table grape at 512 mg L^(−1),while jatrorrhizine(18)exhibited an inhibition rate>90%on grape rot at the same concentration,with lower cytotoxicity and residue than chlorothalonil,which suggested that ingredients of *** might be a low-toxicity,low-residue,eco-friendly botanical fungicide against postharvest pathogens.

关键词： Mahonia fortunei Chemical constituents Botrytis cinerea Penicillium italicum Anti-postharvest pathogens

Association of TCR-signaling pathway with the development of lacrimal gland benign lymphoepithelial lesions

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International Journal of Ophthalmology(English edition) 2015年第4期8卷 685-689页

作者： Jian-Min Ma Yi-Xin Cui Xin Ge Jing Li Jin-Ru Li xiao-na wang Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical University Beijing Ophthalmology &Vision Science Key Lab

·AIM: To identify the association of the T cell receptor(TCR) signaling with the development of benign lymphoepithelial lesions(BLEL) of the lacrimal gland.· METHODS: We collected affected lacrimal gland tissues from 9 patients who underwent dacryoadenectomy in the Capital Medical University Beijing Tongren Hospital Eye Center between August2010 and March 2013 and were confirmed to have lacrimal gland BLEL by histopathological analysis. Tumor tissues from 9 patients with orbital cavernous hemangioma were also collected and used as *** genome gene expression microarray was used to compare gene expression profiles of affected lacrimal gland tissues from patients with lacrimal gland BLEL to those from of orbital cavernous *** expression of TCR pathway genes between these tissues was confirmed by polymerase chain reaction(PCR) and immunohistochemistry.·RESULTS: Microarray analysis showed that in lacrimal glands with BLEL, 32 signaling pathways were enriched in the upregulated genes, while 25 signaling pathways were enriched in the downregulated genes. In-depth analysis of the microarray data showed that the expression of 27 genes of the TCR signaling pathway increased significantly. To verify the differential expression of three of these genes, CD3, CD4, and interleukin(IL)-10, reverse transcription-PCR(RT-PCR)and immunohistochemistry assays were performed. RT-PCR analysis showed that CD3 and CD4 were expressed in the lacrimal glands with BLEL, but IL-10 was not expressed. Immunohistochemistry confirmed that CD3 and CD4 proteins were also present, but IL-10 protein was not. CD3, CD4, or IL-10 expression was not found in the orbital cavernous hemangiomas with either RT-PCR or immunohistochemistry.· CONCLUSION: TCR signaling pathway might be involved in the pathogenesis of lacrimal gland BLEL.

关键词： lacrimal gland benign lymphoepithelial lesion whole genome gene expression microarray T cell receptor-signaling pathway

Characteristics and prognosis of gastric cancer in patients aged ≥ 70 years

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World Journal of Gastroenterology 2013年第39期19卷 6568-6578页

作者： Yue-Xiang Liang Jing-Yu Deng Han-Han Guo Xue-Wei Ding xiao-na wang Bao-Gui wang Li Zhang Han Liang Department of Gastric Cancer Tianjin Medical University Cancer Institute and HospitalKey Laboratory of Cancer Prevention and TherapyNational Clinical Research Center of Cancer Department of Cardiovascular Surgery the First Hospital of Tianshui City

AIM:To elucidate the prognostic value of age for gastric cancer and identify the optimal treatment for elderly gastric cancer ***:We enrolled 920 patients with gastric cancer who underwent gastrectomy between January 2003and December 2007 in our *** were categorized into three groups:younger group(agenal hazards regression *** analysis was used to investigate the impact of age on survival at each ***-specific survival was also compared among the three groups by excluding deaths due to reasons other than gastric *** analyzed the potential prognostic factors for patients aged≥***,the impact of extent of lymphadenectomy and postoperative chemotherapy on survival for each age group was ***:In the elderly group,there was a male *** the same time,cancers of the upper third of the stomach,differentiated type,and less-invasive surgery were more common than in the younger or middle-aged *** patients were more likely to have advanced tumor-node-metastasis(TNM)stage and larger tumors,but less likely to have distant *** 5-year overall survival(OS)rate specific to gastric cancer was not significantly different among the three groups,elderly patients demonstrated a significantly lower 5-year OS rate than the younger and middle-aged patients(elderly vs middle-aged vs younger patients=22.0%vs 36.6%vs 38.0%,respectively).In the TNM-stratified analysis,the differences in OS were only observed in patients withⅡandⅢ*** multivariate analysis,only surgical margin status,pT4,lymph node metastasis,M1 and sex were independent prognostic factors for

关键词： Gastric carcinoma Elderly patients Prognosis Lymphadenectomy Chemotherapy