MdbHLH162 connects the gibberellin and jasmonic acid signals to regulate anthocyanin biosynthesis in apple

作     者：Jian-Ping An Rui-Rui Xu Xiao-Na Wang Xiao-Wei Zhang Chun-Xiang You Yuepeng Han Jian-Ping An;Rui-Rui Xu;Xiao-Na Wang;Xiao-Wei Zhang;Chun-Xiang You;Yuepeng Han

作者机构：Apple technology innovation center of Shandong ProvinceCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-An 271018China CAS Key Laboratory of Plant Germplasm Enhancement and Specialty AgricultureWuhan Botanical GardenHubei Hongshan LaboratoryThe Innovative Academy of Seed Design of Chinese Academy of SciencesWuhan 430074China College of Biology and OceanographyWeifang UniversityWeifang 261061China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2024年第66卷第2期

页      面：265-284页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：financially supported by grants from the Natural Science Foundation of Shandong Province(ZR2022YQ24) the Development Plan of the Youth Innovation Team of the Higher Education Institutions in Shandong Province(2022KJ326) Wuhan Botanical Garden Scientific Research Support Project(E3559901) 

主　　题：anthocyanin biosynthesis bHLH transcription factor gibberellin jasmonic acid regulatory network transcriptional regulation 

摘      要：Anthocyanins are secondary metabolites induced by environmental stimuli and developmental *** positive regulators of anthocyanin biosynthesis have been reported,whereas the anthocyanin repressors have been *** the signal transduction pathways of gibberellin(GA)and jasmonic acid(JA)and their regulation of anthocyanin biosynthesis have been investigated,the cross-talk between GA and JA and the antagonistic mechanism of regulating anthocyanin biosynthesis remain to be *** this study,we identified the anthocyanin repressor MdbHLH162 in apple and revealed its molecular mechanism of regulating anthocyanin biosynthesis by integrating the GA and JA ***162 exerted passive repression by interacting with MdbHLH3 and MdbHLH33,which are two recognized positive regulators of anthocyanin ***162 negatively regulated anthocyanin biosynthesis by disrupting the formation of the anthocyanin-activated MdMYB1-MdbHLH3/33complexes and weakening transcriptional activation of the anthocyanin biosynthetic genes MdDFR and MdUF3GT by MdbHLH3 and *** GA repressor MdRGL2a antagonized MdbHLH162-mediated inhibition of anthocyanins by sequestering MdbHLH162 from the MdbHLH162-MdbHLH3/33 *** JA repressors MdJAZ1 and MdJAZ2 interfered with the antagonistic regulation of MdbHLH162 by MdRGL2a by titrating the formation of the MdRGL2a-MdbHLH162 *** findings reveal that MdbHLH162 integrates the GA and JA signals to negatively regulate anthocyanin *** study provides new information for discovering more anthocyanin biosynthesis repressors and explores the cross-talk between hormone signals.