The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple
作者机构：National Key Laboratory of Crop BiologyMOA Key Laboratory of Horticultural Crop Biology and Germplasm InnovationCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’An271018ShandongChina College of Life ScienceShandong Agricultural UniversityTai’An271018ShandongChina
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2017年第4卷第1期
页 面：246-254页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：This work was supported by grants from the Natural Science Foundation of China(31325024 and 31601742) the Ministry of Education of China(IRT15R42)and Shandong Province Government(SDAIT-06-03)
主 题：HY5 nitrate assimilation
摘 要：The basic leucine zipper(bZIP)transcription factor HY5 plays a multifaceted role in plant growth and *** the apple MdHY5 gene was cloned based on its homology with Arabidopsis *** analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid *** mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs,***,we obtained transgenic apple calli that overexpressed the MdHY5 gene,and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis *** addition,the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were *** association with increased nitrate reductase activities and nitrate contents,the results indicated that MdHY5 might be an important regulator in nutrient *** together,these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.
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