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Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure

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Science China Chemistry 2011年第8期54卷 1273-1276页

作者： GE ZhiLei PEI Hao WANG LiHua song shiping FAN ChunHai Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China

An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM.

关键词： DNA nanostructure surface density electrochemical

新型冠状病毒感染疫情下在校医学生手卫生知信行现状调查

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河南中医 2024年第1期44卷 102-107页

作者：宋苑乔娟徐碧璇程宇晗宋世平王钲皓河南中医药大学河南郑州450046

目的:探讨新型冠状病毒感染疫情下在校医学生手卫生现状及影响因素。方法:2020年9—11月在21个专业中随机抽取护理学专业和针灸推拿学专业医学生,最终确定调查样本量为240份。以知信行模式为理论依据,设计《在校医学生手卫生知信行调查... 详细信息

目的:探讨新型冠状病毒感染疫情下在校医学生手卫生现状及影响因素。方法:2020年9—11月在21个专业中随机抽取护理学专业和针灸推拿学专业医学生,最终确定调查样本量为240份。以知信行模式为理论依据,设计《在校医学生手卫生知信行调查问卷》,问卷内容包括知识、态度和行为三个维度,22个条目,知识、态度和行为三个维度的Cronbach′sα系数分别为0.67、0.70、0.76,重测信度分别为0.74、0.85、0.67。对在校医学生进行手卫生知信行现状问卷调查,采用生物荧光监测法测量手部菌落,采用SPSS 19.0软件对数据进行描述和分析。结果:本次调查共发放问卷240份,回收有效问卷232份,有效回收率为96.67%。232名在校医学生手卫生知识平均知晓率为76.73%,85.4%医学生认为通过自己的努力有信心做好手卫生,66%的在校医学生认为自己手卫生做得好或很好。在校医学生手卫生后手部菌落值为(52.36±55.08),手卫生达标率为41.81%。专业、手卫生培训内容和手卫生自我效能3个变量与手卫生均落值成共线性关系,共解释变异度的35.8%(t=-2.714~2.804,P=0.005~0.028.R^(2)=0.358,F=11.206,P=0.000<0.05)。结论:在校医学生手卫生知晓率和自我效能较高,但手卫生达标率不容乐观,护理专业、接受过手卫生培训和手卫生自我效能高者依从率高,可从转变学生的信念入手,进行教育和强化,促使学生养成良好的手卫生行为习惯。

关键词：新型冠状病毒感染手卫生知信行现状在校医学生

Light-harvesting Polymer Based DNA Assay for SNP Identification of BRCA1 Mutations

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稀有金属材料与工程 2006年第A3期35卷 274-276页

作者： Jiang Li Xingfen Liu Hui Xu shiping song Yun Zhao Chunhai Fan School of Life Science Sichuan University Chengdu China Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai China

We report an improved strategy for conjugated polymer-based optical DNA detection with stem-loop structured DNA *** high sensitivity of conjugated polymer-based biosensors arises from light harvesting by the conjugated polymer and the related amplified fluorescent signal transduction either through quenching or through Fǒrster energy transfer) In our previous publication,we demonstrated that the coupling of magnetic particles to conjugated polymers significantly improves the selectivity of this class of DNA *** we further improved the selectivity by introducing both stem-loop structured capture probe and competition *** demonstrated further that this improved magnetically assisted DNA sensor can conveniently identify even a single-nucleotide mismatch in the target sequence.

关键词： conjugated polymers DNA detection BRCA1 SNF identification

Design of a Carbon Nanotube/Magnetic Nanoparticle-Based Peroxidase-Like Nanocomplex and Its Application for Highly Efficient Catalytic Oxidation of Phenols

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Nano Research 2009年第8期2卷 617-623页

作者： Xiaolei Zuo Cheng Peng Qing Huang shiping song Lihua Wang Di Li Chunhai Fan Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China

We report a novel nanotechnology-based approach for the highly efficient catalytic oxidation of phenols and their removal from *** use a nanocomplex made of multi-walled carbon nanotubes(MWNTs)and magnetic nanoparticles(MNPs).This nanocomplex retains the magnetic properties of individual MNPs and can be effectively separated under an external magnetic fi *** importantly,the formation of the nanocomplex enhances the intrinsic peroxidase-like activity of the MNPs that can catalyze the reduction of hydrogen peroxide(H2O2).Significantly,in the presence of H2O2,this nanocomplex catalyzes the oxidation of phenols with high effi ciency,generating insoluble polyaromatic products that can be readily separated from water.

关键词： Carbon nanotubes magnetic nanoparticles phenol peroxidase catalysis

The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

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Science China Chemistry 2009年第6期52卷 746-750页

作者： ZHANG LanYong WAN Ying ZHANG Jiong LI Di WANG LiHua song shiping FAN ChunHai Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China

A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H2O2, leading to a limit of detection as low as 10 fM.

关键词： DNA detection conducting polymer HRP electrochemistry

A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin

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Nuclear Science and Techniques 2012年第5期23卷 317-320页

作者： ZHENG Xiaoxue WEN Yanqin ZHANG Juan WANG Lihua song shiping ZHANG Hua FAN Chunhai Shanghai Institute of Applied Physics Chinese Academy of Sciences Jiading Campus Shanghai 201800 China School of Materials Science and Engineering Nanyang Technological University 639798 Singapore

Resonance light scattering (RLS) is a sensitive technique for monitoring scattered light induced by extended aggregates of chromophores. It has been widely used to study aggregations for its simple manipulation, high sensitivity and great versatility. Gold nanoparticles generate colorful light-scattering signals due to their unique surface plasmon resonances, hence extraordinary light scattering upon aggregation. In this paper we report a rapid and sensitive method based on gold nanoparticles and DNA aptamer to detect protein biomarkers by RLS. Thiol modified thrombin aptamer was covalently assembled to the surface of gold nanoparticles as nanobio probes. As thrombin has two specific binding sites for its aptamer, it can bridge the well dispersed nanoparticles and lead to a network of particle aggregations. The formation of aggregation ia measured by RLS, and the specific detection of thrombin at nM level is achieved. The method has good specificity.

关键词：金纳米粒子特异性检测生物探针凝血酶灵敏度表面等离子共振适配体共振光散射

基于生物信息学方法设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗

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黑龙江畜牧兽医 2024年第18期 104-109,116,135,136页

作者：刘意张喜悦李昕向祝郎雨辰宋时萍赵格曲志娜刘立恒王君玮中国动物卫生与流行病学中心致病微生物监测室山东青岛266032 农业农村部畜禽产品质量安全风险评估实验室(青岛) 山东青岛266032 江西农业大学动物科学技术学院南昌330045

为了设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗,试验在NCBI网站的Protein数据库中获取OmpC和FliC蛋白的氨基酸序列,采用Expasy服务器分析其理化性质,通过在线工具AllerTopV.2.0和Vexijen分析其抗原性和致敏性,应用NPS~@服务器分析二级结构... 详细信息

为了设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗,试验在NCBI网站的Protein数据库中获取OmpC和FliC蛋白的氨基酸序列,采用Expasy服务器分析其理化性质,通过在线工具AllerTopV.2.0和Vexijen分析其抗原性和致敏性,应用NPS~@服务器分析二级结构,应用IEDB在线工具分析OmpC和FliC的B、T细胞表位。选取优势表位设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗,对其理化性质、抗原性、致敏性及三级结构进行预测。结果表明:OmpC和FliC均是亲水性蛋白,无致敏性,抗原性较高;OmpC和FliC蛋白均筛选出3个B细胞优势表位,2个细胞毒性T细胞(CTL细胞)优势表位,1个辅助T细胞(Th细胞)优势表位。设计的多表位疫苗为亲水性的稳定蛋白,抗原性预测的分数为1.1422分,且为非致敏原,预测三级结构模型的可信度大。说明鼠伤寒沙门氏菌OmpC-FliC多表位疫苗对动物无致敏性,且具有良好的抗原性。

关键词：沙门氏菌生物信息学 OmpC FliC 表位疫苗

A Nano-and Micro-Integrated Protein Chip Based on Quantum Dot Probes and a Microfluidic Network

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Nano Research 2008年第6期1卷 490-496页

作者： Juan Yan Mei Hu Di Li Yao He Rui Zhao Xingyu Jiang shiping song Lianhui Wang Chunhai Fan Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Laboratory of Advanced Materials(LAM) Fudan University220 Handan RoadShanghai 200433China National Center for NanoScience and Technology No.11BeiyitiaoZhongguancunBeijing 100080China

A novel nano-and micro-integrated protein chip(NMIPC)that can detect proteins with ultrahigh sensitivity has been fabricated.A microfl uidic network(μFN)was used to construct the protein chips,which allowed facile patterning of proteins and subsequent biomolecular *** phase-synthesized,water-soluble fl uorescent CdTe/CdS core-shell quantum dots(aqQDs),having high quantum yield and high photostability,were used as the signaling ***,it was found that aqQDs were compatible with microfluidic format assays,which afforded highly sensitive protein chips for cancer biomarker assays.

关键词： Quantum dots microfl uidics carcinoma embryonic antigen(CEA) microarray polydimethylsiloxane(PDMS)

An organic free radical generator independent on hydrogen peroxide for chemodynamic and immunotherapy in vivo

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Journal of Materials Science & Technology 2024年第7期174卷 85-94页

作者： Yang Chen Yunpeng song Chaojie Tang Hong Yang Wu Wang shiping Yang Zhiguo Zhou College of Chemistry and Materials Science International Joint Laboratory on Resource Chemistry of Ministry EducationShanghai Frontiers Science Center of Biomimetic CatalysisShanghai Normal UniversityShanghai 200234China Institute of Diagnostic and Interventional Radiology Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China

It is desirable to develop a free radical generator that is neither dependent on hydrogen peroxide nor external stimuli-triggered to achieve effective tumor ***,the free radical genera-tor MNPs-mCPBA was constructed with Zn_(0.4)Co_(0.6)F_(e2)O_(4)@Zn_(0.4)Mn_(0.6)Fe_(2)O_(4)nanoparticles(MNPs)and 3-chloroperbenzoic acid(mCPBA).We believe that the iron(Ⅱ)on the surface of MNPs-mCPBA maybe represent high reactive activity and promote the generation of a large number of organic free radicals(C_(6)H_(4)ClCOOO)in an acidic tumor microenvironment,which realizes the acid-dependent tumor treat-ment strategy independent on the H_(2)O_(2)level in *** addition,the generated organic free radicals(C_(6)H_(4)ClCOOO)effectively induced immunogenic cell death(ICD)and increased T cell ***-der the synergetic treatment with an anti-PD-L1 antibody(α-PD-L1),a favorable anti-tumor effect was achieved.

关键词： Organic free radicals Immunogenic cell death Immunotherapy Chemodynamic therapy