The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

作     者：ZHANG LanYong WAN Ying ZHANG Jiong LI Di WANG LiHua SONG ShiPing FAN ChunHai 

作者机构：Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2009年第52卷第6期

页      面：746-750页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 20725516, 20704043, 20873175 & 20805055) Shanghai Municipal Commission for Science and Technology (Grant Nos. 0752nm021 & 07ZR14136) Ministry of Sci-ence and Technology (Grant Nos. 2006CB933000, 2007CB936000 & 2007AA06A406) Ministry of Health (Grant No. 2009ZX10603) China Post-doctoral Science Foundation and Shanghai Postdoctoral Scientific Program (Grant No. 07R214160) 

主　　题：DNA detection conducting polymer HRP electrochemistry 

摘      要：A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H2O2, leading to a limit of detection as low as 10 fM.