Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure

作     者：GE ZhiLei PEI Hao WANG LiHua SONG ShiPing FAN ChunHai 

作者机构：Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2011年第54卷第8期

页      面：1273-1276页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 071007[理学-遗传学] 0703[理学-化学] 

基　　金：support from the National Natural Science Foundation of China, Ministry of Health (2009ZX10004-301) Science and Technology Commission of Shanghai Munipality (0952nm04600) 

主　　题：单核苷酸多态性 电化学检测 DNA检测 表面检测 纳米结构 基因分型 固定化 DNA杂交 

摘      要：An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM.