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Characterization and expression pattern of pouII1,a novel class Ⅱ POU gene in zebrafish

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Chinese Science Bulletin 2001年第18期46卷 1523-1527,1586页

作者： LI Ming CAO Ying ZHAO Zhixing lin shuo MENG Anming Institute of Cellular and Developmental Biology Department of Biological Science and Biotechnologyand the Protein Science Laboratory of MOETsinghua UniversityBeijing 100084China Institute for Molecular Medicine and Genetics Medical College of GeorgiaGA30912USA

POU domain transcription factors that share a conserved DNA-binding domain, POU domain, are important regulators for the development of embryos in various animal species. A novel zebrafish POU domain gene, poulll has been cloned. The poullll cDNA is 2080 kb in length and encodes a putative polypeptide of 596 ammo acids. It is placed into class II POU family since it shares a high degree of homology with the known members of this family. Northern hybridization identifies a major transcript of approximately 2.1 kb that was present in embryos at the single-cell stage throughout 24 h postfertilization. The whole mount in situ hybridization shows that poulll transcripts are present in the single-cell embryos, strongly suggesting that these transcripts are of maternal origin. During early development of the embryos, poulll mRNA was ubiquitously distributed in all cells and tissues. The transcripts are gradually limited to brains and become completely undetectable by day 3. To our knowledge, poulll is the

关键词： POU zebrafish maternal gene embryogenesis embryos expression pattern.

Optimized Target-AID system efficiently induces single base changes in zebrafish

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Journal of Genetics and Genomics 2018年第4期45卷 215-217页

作者： Xiaochan Lu Yunxing Liu Guanrong Yan Song Li Wei Qin shuo lin State Key Laboratory of Chemical Oncogenomics Key Laboratory of Chemical Genomics Peking University Shenzhen Graduate School Department of Molecular Cell and Development Biology University of CaliforniaLos Angeles

CRISPR-Cas system has been widely adapted as a platform for genome editing in various eukaryotic organisms, including zebrafish（Cong et al., 2013; Hwang et al., 2013）. One of the important applications of CRISPR-Cas... 详细信息

关键词： AID Optimized Target-AID system efficiently induces single base changes in zebrafish Target

“双碳”背景下能源动力类实验室安全文化与制度研究

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教育教学论坛 2024年第3期 5-8页

作者：杨燕茹张凌云王硕林华北电力大学能源电力创新研究院北京102206 内蒙古工业大学能源与动力工程学院内蒙古呼和浩特010051

“双碳”背景下,我国能源发展面临新态势与新挑战,能源领域对相关从业人员也提出了新标准与新需求。能源动力类实验室作为培养能源动力类本科生及研究生能力的重要阵地,其在培养过程中的作用与重要性日益凸显。然而,由于能源动力类实验... 详细信息

“双碳”背景下,我国能源发展面临新态势与新挑战,能源领域对相关从业人员也提出了新标准与新需求。能源动力类实验室作为培养能源动力类本科生及研究生能力的重要阵地,其在培养过程中的作用与重要性日益凸显。然而,由于能源动力类实验室内部环境的特殊性和实验操作的复杂性,实验室的安全管理面临一系列挑战。从“双碳”背景对能源动力类毕业生的要求出发,结合能源动力类实验室自身职责和特点,围绕如何构建实验室安全文化,如何完善实验室安全体系,如何从思想认识和制度保障上提高实验室安全管理水平,以保障实验室人员人身安全和实验设备正常运行等方面进行了论述与研究,为实验室安全管理思路的拓展提供了新角度和新视野。

关键词：能源动力类实验室安全文化建设制度完善

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

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Cell Regeneration 2015年第1期4卷 1-7页

作者： Anne G lindgren Matthew B Veldman shuo lin Department of Molecular Cellular and Developmental Biology University of California615 Charles E.Young Drive SouthLos AngelesCA 90095USA

Background:Endothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the *** variant 2(ETV2)is transiently expressed in both zebrafish and mice and is necessary and sufficient for vascular endothelial cell *** of this gene in early zebrafish and mouse embryos results in ectopic appearance of endothelial *** expression of ETV2 in later development results in only a subset of cells responding to the ***:We have examined the expression pattern of ETV2 in differentiating human embryonic stem cells(ESCs)to determine when the peak of ETV2 expression *** show that overexpression of ETV2 in differentiating human ESC is able to increase the number of endothelial cells generated when administered during or after the endogenous peak of gene ***:Addition of exogenous ETV2 to human ESCs significantly increased the number of cells expressing angioblast genes without arterial or venous *** may be a viable solution to generate in vitro endothelial cells for use in research and in the clinic.

关键词： Human embryonic stem cells Endothelium ETV2 Differentiation

Local chemical ordering coordinated thermal stability of nanograined high-entropy alloys

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Rare Metals 2023年第5期42卷 1645-1655页

作者： Hong-Hui Wu lin-shuo Dong Shui-Ze Wang Gui-lin Wu Jun-Heng Gao Xu-Sheng Yang Xiao-Ye Zhou Xin-Ping Mao Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing100083China Yangjiang Branch Guangdong Laboratory for Materials Science and Technology(Yangjiang Advanced Alloys Laboratory)Yangjiang529500China Department of Industrial and Systems Engineering The Hong Kong Polytechnic UniversityKowloon999077Hong KongChina Guangdong Province Key Laboratory of Durability for Marine Civil Engineering School of Civil EngineeringShenzhen UniversityShenzhen518060China

Nanograined(NG)materials often suffer from low thermal stability owing to the high volume fraction of grain boundaries(GBs).Herein,we investigate the possibility of utilizing local chemical ordering(LCO)for improving the thermal stability of NG FeCoNiCrMn highentropy alloys(HE As).NG HE As with two different grain sizes were *** tests and creep test simulations were then performed to reveal the influence of LCO on the mechanical properties and thermal stability of NG HE *** performing hybrid molecular dynamics and Monte Carlo simulations,Cr atoms were found to accumulate at *** analyzing the atomic structure evolution during the deformation process,we found that the formation of LCO effectively stabilized the GBs and inhibited GB *** addition,dislocation nucleation from GBs and dislocation movement was also *** inhibiting effect of LCO on GB movement and dislocation activity is more prominent than in the NG model with smaller grain *** current simulation results suggest a possible strategy for enhancing the thermal stability of NG HEAs for service in a high-temperature environment.

关键词： High-entropy alloys(HEAs) Local chemical ordering(LCO) Molecular dynamics(MD)simulation Monte Carlo(MC)approach

生物质灰调理剂对烟田土壤性质及烟草生长和养分含量及烟叶品质的影响

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安徽农业大学学报 2024年第2期51卷 297-304页

作者：王丽何东东朱启法李世金林硕薛琳康治东代维祥李硕江彤章力干安徽农业大学资源与环境学院农田生态保育与污染防控安徽省重点实验室合肥230036 安徽农业大学植物保护学院合肥230036 安徽皖南烟叶有限责任公司宣城242000

通过田间试验,研究生物质灰调理剂对烟田土壤化学性质、烟草生长及其烤烟品质的影响。试验设5个处理:不施肥(CK1)、常规施肥(CK2)、常规施肥配施750 kg·hm^(-2)调理剂(T1)、常规施肥配施1500 kg·hm^(-2)调理剂(T2)和常规施肥配施2250... 详细信息

通过田间试验,研究生物质灰调理剂对烟田土壤化学性质、烟草生长及其烤烟品质的影响。试验设5个处理:不施肥(CK1)、常规施肥(CK2)、常规施肥配施750 kg·hm^(-2)调理剂(T1)、常规施肥配施1500 kg·hm^(-2)调理剂(T2)和常规施肥配施2250 kg·hm^(-2)调理剂。结果表明:调理剂施用显著提高了烟草根区土壤pH值、矿质态氮、速效磷和速效钾含量。与CK2相比,调理剂处理(T1—T3)根区pH提高了0.36~0.44,硝态氮增幅达49.6~243.5%,矿质态氮增幅为26.5%~164.6%。其中,T2处理可显著提高烟草生长中期根区硝态氮含量,而显著降低其成熟期根区硝态氮含量,利于烟草生长后期烟叶的落黄。与CK2相比,调理剂配施(T1—T3)显著促进烟草根系生长发育,提高烟株茎粗和中前期烟叶氮磷钾含量,增加烤烟产量,其增幅为3.6%~6.2%;同时,烤烟上部叶钾含量增加,氮和还原糖含量降低,利于烤烟中部叶糖碱比的提升和烤烟品质的改善。综上,生物质灰调理剂的添加,可改良植烟土壤化学性质和养分供给,提升烤烟养分积累,改善烤烟品质,并以1500 kg·hm^(-2)为最佳施用量。

关键词：烟草调理剂土壤养分烟草品质

Association of serum lipoprotein(a) level with the severity and prognosis of calcific aortic valve stenosis: a Chinese cohort study

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Journal of Geriatric Cardiology 2020年第3期17卷 133-140页

作者： shuo-lin LIU Rynat Rozi Hui-Wei SHI Ying GAO Yuan-lin GUO Yi-Da TANG Jian-Jun LI Na-Qiong WU Endocrinology&Cardiometabolic Center State Key Laboratory of Cardiovascular DiseaseFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Background There was a causal relationship between elevated lipoprotein(a)[Lp(a)]levels and increased risk of calcific aortic valve stenosis(CAVS)in whites and *** present study aimed to investigate whether Lp(a)levels were associated with aortic stenosis(AS)severity and clinical events in Chinese *** Levels of serum Lp(a)were measured in 652 patients with CAVS,whom all underwent baseline echocardiographic *** clinical endpoint was defined as a composite of aortic valve replacement(AVR)and cardiac *** Patients in the tertile 3 of Lp(a)had a higher percentage of severe AS compared with those in the tertile 1 and 2 of Lp(a)(46.2%vs.33.9%,P=0.005).Moreover,the top tertile of Lp(a)was an independent predictor of severe AS(OR=1.78,95%CI:1.18-2.66,P=0.006).However,there was no significant association between tertile 3 of Lp(a)and clinical events(hazard ratio:0.73;95%CI:0.43-1.24;P=0.239)in the multivariate Cox regression analysis during a mean follow-up time of 3.16±2.74 *** Elevated Lp(a)level was an independent predictor of severe AS by echocardiography in the Chinese population,but was not associated with the increased risk of AVR and cardiac death,suggesting that Lp(a)levels might be helpful in the risk stratification of patients with CAVS.

关键词： Aortic stenosis Calcification Severity Lipoprotein(a) Prognosis

不同无创通气联合枸橼酸咖啡因治疗早产儿呼吸暂停疗效分析

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医学研究杂志 2024年第1期53卷 147-150,4页

作者：林硕罗厚江杨军贾文婷汪灵芝沈怀云蚌埠医学院第二附属医院新生儿科 233000

目的通过分析不同无创辅助通气模式联合枸橼酸咖啡因在早产儿呼吸暂停中的治疗效果,探讨治疗早产儿呼吸暂停的优化方案。方法选择2019年10月~2022年8月蚌埠医学院第二附属医院新生儿重症监护病房收治的90例胎龄≤34周的呼吸暂停患儿作... 详细信息

目的通过分析不同无创辅助通气模式联合枸橼酸咖啡因在早产儿呼吸暂停中的治疗效果,探讨治疗早产儿呼吸暂停的优化方案。方法选择2019年10月~2022年8月蚌埠医学院第二附属医院新生儿重症监护病房收治的90例胎龄≤34周的呼吸暂停患儿作为研究对象,回顾性分析患儿的病例资料,将患儿分为NCPAP联合枸橼酸咖啡因组(n=44)和NIPPV序贯NCPAP联合枸橼酸咖啡因组(n=46)。采用t检验、χ2检验、Mann-Whitney检验等,对两组患儿一般临床资料、呼吸暂停疗效、治疗时间及不良反应发生情况,进行统计学比较。结果NIPPV序贯NCPAP联合枸橼酸咖啡因组治疗早产儿呼吸暂停总有效率为91.30%,高于NCPAP联合枸橼酸咖啡因组的86.36%,差异有统计学意义(P<0.05);两组无创通气失败及相关治疗时间比较,差异有统计学意义(P<0.05);两组在喂养不耐受、鼻损伤发生情况比较,差异有统计学意义(P<0.05)。结论早期采用NIPPV序贯NCPAP的通气模式,较传统单用NCPAP治疗早产儿呼吸暂停,可以取得更好的临床效果,缩短治疗及住院时间,不良反应相对更少,值得临床推广。

关键词：早产儿早产儿呼吸暂停枸橼酸咖啡因经鼻持续气道正压通气经鼻间歇正压通气

智能化辅助采集系统在临床生命体征监测中的应用

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中国数字医学 2024年第5期19卷 48-52页

作者：董家凡翁雅丽陈海鸿林硕林敏黄秋红王继伟陆军第七十三集团军医院(厦门大学附属成功医院)信息科福建厦门361003 陆军第七十三集团军医院(厦门大学附属成功医院)护理部福建厦门361003

目的:减少临床护士对患者体征测量不及时、护理文书内容不准确等一系列问题,辅助临床护士进行患者每日体温、脉搏等生命体征数据采集。方法:开发一套与医院护理信息系统结合的生命体征辅助采集系统,利用系统自动获取的患者医嘱、病情、... 详细信息

目的:减少临床护士对患者体征测量不及时、护理文书内容不准确等一系列问题,辅助临床护士进行患者每日体温、脉搏等生命体征数据采集。方法:开发一套与医院护理信息系统结合的生命体征辅助采集系统,利用系统自动获取的患者医嘱、病情、手术记录等个人信息,并与护理规范相适应,通过算法智能化得出患者每日体征测量类型、时间、频次,并及时提示操作者。结果:该系统应用后,临床护士个人护理操作不规范、护理文书填写不及时等问题明显减少,护士每日完成护理文书记录时长明显缩短,科室护理文书完整率明显提高,差异具有统计学意义(P<0.05),大大提高了护士工作效率与护理工作质量。结论:智能化生命体征辅助采集系统提高了护理文书书写的完整性、及时性,用户体验好。

关键词：护理文书生命体征辅助采集