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Development of genome engineering technologies in cattle: from random to specific

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Journal of Animal Science and Biotechnology 2018年第2期9卷 285-293页

作者： Soo-young Yum Ki-young Youn Woo-Jae Choi Goo jang Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University Farm Animal Clinical Training and Research Center Institute of Green Bio Science Technology Seoul National University Emergence Center for Food-Medicine Personalized Therapy System Advanced Institutes of Convergence Technology Seoul National University College of Veterinary Medicine Seoul National University

The production of transgenic farm animals(e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into one-cell stage embryos(zygotes) followed by embryo transfer into a recipient was performed because of the ease of the ***, as this approach resulted in severe mosaicism and has a low efficiency, it is not typically employed in the cattle as priority, unlike in mice. To overcome the above issue with micro-injection in cattle, somatic cell nuclear transfer(SCNT) was introduced and successfully used to produce cloned livestock. The application of SCNT for the production of transgenic livestock represents a significant advancement, but its development speed is relatively slow because of abnormal reprogramming and low gene targeting efficiency. Recent genome editing technologies(e.g.,ZFN, TALEN, and CRISPR-Cas9) have been rapidly adapted for applications in cattle and great results have been achieved in several fields such as disease models and bioreactors. In the future, genome engineering technologies wil accelerate our understanding of genetic traits in bovine and wil be readily adapted for bio-medical applications in cattle.

关键词： Cattle CRISPR-Cas9 Genome engineering technologies Transgenesis Transposon

Tribological properties of multilayer tetrahedral amorphous carbon coatings deposited by filtered cathodic vacuum arc deposition

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Friction 2021年第5期9卷 1292-1302页

作者： young-Jun jang Jae-Il KIM Wooyoung LEE Jongkuk KIM Department of Extreme Environmental Coatings Surface Technology DivisionKorea Institute of Materials Science(KIMS)Gyeongnam-do 51508Republic of Korea Department of Mechanical Science and Engineering Graduate School of EngineeringNagoya UniversityNagoya 464-8603Japan

Tetrahedral amorphous carbon(ta‐C)has emerged as an excellent coating material for improving the reliability of application components under high normal ***,we present the results of our investigations regarding the mechanical and tribological properties of a 2‐μm‐thick multilayer ta‐C coating on high‐speed steel *** composed of alternating soft and hard layers are fabricated using filtered a cathodic vacuum arc with alternating substrate bias voltages(0 and 100 V or 0 and 150 V).The thickness ratio is discovered to be 1:3 for the sp2‐rich and sp3‐rich *** results show that the hardness and elastic modulus of the multilayer ta‐C coatings increase with the sp3 content of the hard *** hardness reached approximately 37 GPa,whereas an improved toughness and a higher adhesion strength(>29 N)are *** friction performance(μ=0.07)of the multilayer coating is similar to that of the single layer ta‐C thick coating,but the wear rate(0.13×10^(–6) mm^(3)/(N∙m))improved under a high load of 30 *** further demonstrate the importance of the multilayer structure in suppressing crack propagation and increasing the resistance to plastic deformation(H3/E2)ratio.

关键词： tetrahedral amorphous carbon(ta‐C) filtered cathodic vacuum arc deposition multilayer coatings alternating substrate bias voltage wear resistance plastic deformation resistance

Expression of a High Mobility Group Protein Isolated from Cucumis sativus Affects the Germination of Arabidopsis thaliana under Abiotic Stress Conditions

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Journal of Integrative Plant Biology 2008年第5期50卷 593-600页

作者： Ji young jang Kyung Jin Kwak Hunseung Kang Department of Plant Biotechnology Agricultural Plant Stress Research Center and Biotechnology Research InstituteCollege of Agriculture and Life SciencesChonnam National UniversityGwangju 500-757Korea

Although high mobility group B （HMGB） proteins have been identified from a variety of plant species, their importance and functional roles in plant responses to changing environmental conditions are largely unknown. Here, we investigated the functional roles of a CsHMGB isolated from cucumber （Cucumis sativus L.） in plant responses to environmental stimuli. Under normal growth conditions or when subjected to cold stress, no differences in plant growth were found between the wild-type and transgenic Arabidopsis thaliana overexpressing CsHMGB. By contrast, the transgenic Arabidopsis plants displayed retarded germination compared with the wild-type plants when grown under high salt or dehydration stress conditions. Germination of the transgenic plants was delayed by the addition of abscisic acid （ABA）, implying that CsHMGB affects germination through an ABA-dependent way. The expression of CsHMGB had affected only the germination stage, and CsHMGB did not affect the seedling growth of the transgenic plants under the stress conditions. The transcript levels of several germination-responsive genes were modulated by the expression of CsHMGB in Arabidopsis. Taken together, these results suggest that ectopic expression of a CsHMGB in Arabidopsis modulates the expression of several germinationresponsive genes, and thereby affects the germination of Arabidopsis plants under different stress conditions.

关键词： abiotic stress cucumber Cucumis sativus high mobility group protein transgenic Arabidopsis plant.

Mechanical and electrochemical characteristics evaluation in annealing treatment for ship material

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中国有色金属学会会刊：英文版 2009年第B9期19卷 40-44页

作者： Seok-Ki jang young-Jae JEONG Seong-Jong KIM Division of Marine Engineering Mokpo Maritime University Jeonnam Techno Park

The effect of heat treatment on the electrochemical and mechanical properties of SS400 steel was investigated, and the effects of annealing conditions on notched specimens subjected to the slow strain rate test (SSRT) were studied. The results show that no correlations are observed among the maximum tensile strength, yield strength, and stress corrosion cracking. In contrast, the elongation and time-to-fracture are improved with stress-relieving annealing compared with the as-received condition. The elongation, time-to-fracture, and number of dimples increase with annealing heat treatment.

关键词：退火处理电化学船舶材料特性评价机械 SS400钢应力腐蚀开裂消除应力退火

Properties of ZnO Thin Films Grown on Si (100) Substrates by Pulsed Laser Deposition

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Journal of Materials Science & Technology 2010年第11期26卷 973-976页

作者： young Rae jang Keon-Ho Yoo Seung Min Park Department of Physics Kyung Hee University Department of Chemistry Kyung Hee University

ZnO thin films were grown on Si （100） substrates by pulsed laser deposition using a ZnO *** substrate temperature was varied in the range of room temperature to 800 ℃,and the oxygen partial pressure of 0.1333 Pa （1 m Torr） to 1333 Pa （10 Torr）.The properties of the resulting films were investigated by photoluminescence （PL）,grazing incidence X-ray diffraction （GIXRD）,X-ray photoelectron spectroscopy （XPS）,and field emission scanning electron microscopy （FESEM）.Based on the ultraviolet （UV,～380 nm） to visible emission ratio in the PL spectrum,the optimum growth conditions were determined to be 600 ℃ and 133.3 Pa （1 Torr）,*** oxygen 1s peak in the XPS spectrum was decomposed into two *** peak at lower binding energy increased in intensity with the oxygen partial pressure from 0.1333 Pa （1 m Torr） to 133.3 Pa （1 Torr） while the other peak *** GIXRD curve at the optimum condition showed strong two peaks （002） and （103）.A strong correlation between the （103） peak intensity and the UV emission intensity was found.

关键词： ZnO Pulsed laser deposition Photoluminescence

Mononuclear cells from the cord blood and granulocytecolony stimulating factor-mobilized peripheral blood:is there a potential for treatment of cerebral palsy?

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Neural Regeneration Research 2015年第12期10卷 2018-2024页

作者： Hani Koh Kyoujung Hwang Hae-young Lim Yong-joo Kim young-Ho Lee Department of Translational Medicine Graduate School of Biomedical Science & EngineeringHanyang University Greencrosscell Corp Analytical Instrumentation Center Medical Branch Hanyang University Department of Pediatrics Hanyang University College of Medicine Cell Therapy Center Hanyang University Medical Center

To investigate a possible therapeutic mechanism of cell therapy in the field of cerebral palsy using granulocyte-colony stimulating factor（G-CSF）-mobilized peripheral blood mononuclear cells（m PBMCs）,we compared the expression of inflammatory cytokines and neurotrophic factors in PBMCs and m PBMCs from children with cerebral palsy to those from healthy adult donors and to cord blood mononuclear cells donated from healthy *** significant differences in expression of neurotrophic factors were found between PBMCs and m ***,in cerebral palsy children,the expression of interleukin-6 was significantly increased in m PBMCs as compared to PBMCs,and the expression of interleukin-3 was significantly decreased in m PBMCs as compared to *** healthy adults,the expression levels of both interleukin-1βand interleukin-6 were significantly increased in m PBMCs as compared to *** expression of brain-derived neurotrophic factors in m PBMC from cerebral palsy children was significantly higher than that in the cord blood or m PBMCs from healthy *** expression of G-CSF in m PBMCs from cerebral palsy children was comparable to that in the cord blood but significantly higher than that in m PBMCs from healthy *** expression of pro-inflammatory cytokines（interleukin-1β,interleukin-3,and-6）and higher expression of anti-inflammatory cytokines（interleukin-8 and interleukin-9）were observed from the cord blood and m PBMCs from cerebral palsy children rather than from healthy *** findings indicate that m PBMCs from cerebral palsy and cord blood mononuclear cells from healthy newborns have the potential to become seed cells for treatment of cerebral palsy.

关键词： neurotrophic factors inflammatory cytokines cord blood G-CSF mobilized peripheralblood mononuclear cell cerebral palsy children neural regeneration

Highly Stable Silicon–Carbon–Nitrogen Composite Anodes from Silsesquiazane for Rechargeable Lithium-Ion Battery

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Journal of Materials Science & Technology 2016年第3期32卷 195-199页

作者： Yong Seok Kim Yong L.joo young-Je Kwark School of Chemical & Biomolecular Engineering Cornell University Department of Organic Materials and Fiber Engineering Soongsil University

Herein, we developed novel silicon-carbon-nitrogen （SiCN） composites synthesized by pyrolyzing silsesquiazane polymer as an anode material for rechargeable lithium-ion batteries. Among variable pyrolysis temperatures of 700 ℃, 1000 ℃ and 1300 ℃, the SiCN composites prepared at 1000 ℃ showed the highest capacity with outstanding battery cycle life by cyclic voltammetry and electrochemical impedance spectroscopy. Such good battery and electrochemical performances should be attributed to a proper ratio of carbon and nitrogen or oxygen in the SiCN composites. Furthermore, our SiCN electrode possessed better lithium ion conductivity than pure silicon nanoparticles. This work demonstrates that polymer-derived composites are among the promising strategies to achieve highly stable silicon anodes for rechargeable batteries.

关键词： Polymer-derived ceramics Silsesquiazane Lithium ion battery Lithium ion conductivity

Deep Drawing Process Using a Tractrix Die for Manufacturing Liners for a CNG High-Pressure Vessel(Type Ⅱ)

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Chinese Journal of Mechanical Engineering 2022年第1期35卷 132-143页

作者： Gun‑young Park Hyo‑Seo Kwak Hyo‑Seong jang Chul Kim School of Mechanical Engineering Pusan National UniversityPusan 46241South Korea Department of Mechanical Engineering Dong-eui UniversityPusan 47340Korea.

The liner of a CNG pressure vessel is manufactured by a DDI(deep drawing and ironing)process for the cylinder part,which is a continuous process that includes a drawing process to reduce the diameter of the billet and a subsequent ironing process to reduce the thickness of the billet.A tractrix die used in the 1^(st) deep drawing allows the blank to flow smoothly by decreasing the punch load and radial tensile stress occurring in the *** also increases the draw ratio compared to conventional dies,but it causes forming *** this study,a shape coefficient(S_(c))is proposed for the tractrix die using the blank diameter(D_(0)),inflow diameter of the workpiece(d_(i)),and inflow angle of the workpiece(θ)for design of the tractrix *** effects of the thickness and inflow angle of the workpiece on wrinkling and folding were investigated through ***,a discriminant is proposed for the relative radial stress(σ)generated during the deep drawing process using the tractirx die and used to predict *** on the results,the blank thickness,the draw ratio,and the inflow of the workpiece angle in the 1^(st) deep drawing process are suggested,and the number of operations in the DDI process was reduced from 6 to *** improves the productivity and reduces the manufacturing cost.

关键词： Tractrix die Deep drawing Wrinkling Folding LDR(Limit draw ratio) FEM

Surface properties of Al-doped ZnO thin film before and after CF_4/Ar plasma etching

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Plasma Science and Technology 2022年第7期24卷 194-200页

作者： young-Hee joo Gwan-Ha KIM Doo-Seung UM Chang-Il KIM School of Electrical and Electronics Engineering Chung-Ang UniversitySeoul 06974Republic of Korea Department of Semiconductor Materials and Applications Korea PolytechnicSeongnam 13122Republic of Korea Department of Electrical Engineering Sejong UniversitySeoul 05006Republic of Korea

Al-doped ZnO(AZO) is considered as an alternative to transparent conductive oxide *** and achieving a stable surface are important challenges in the development and optimization of dry etching processes, which must be overcome for the application of AZO in various devices. Therefore, in this study, the etch rate and surface properties of an AZO thin film after plasma etching using the adaptive coupled plasma system were investigated. The fastest etch rate was achieved with a CF_(4)/Ar ratio of 50:50 sccm. Regardless of the ratio of CF_(4) to Ar,the transmittance of the film in the visible region exceeded 80%. X-ray photoelectron spectroscopy analysis of the AZO thin film confirmed that metal-F bonding persists on the surface after plasma etching. It was also shown that F eliminates O vacancies. Consequently, the work function and bandgap energy increased as the ratio of CF-4 increased. This study not only provides information on the effect of plasma on AZO thin film, but identifies the cause of changes in the device characteristics during device fabrication.

关键词： Al-doped ZnO plasma etching F-based plasma surface characteristics X-ray photoelectron spectroscopy ultraviolet photoelectron spectroscopy