Expression of a High Mobility Group Protein Isolated from Cucumis sativus Affects the Germination of Arabidopsis thaliana under Abiotic Stress Conditions

作     者：Ji Young Jang Kyung Jin Kwak Hunseung Kang 

作者机构：Department of Plant BiotechnologyAgricultural Plant Stress Research Center and Biotechnology Research InstituteCollege of Agriculture and Life SciencesChonnam National UniversityGwangju 500-757Korea 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2008年第50卷第5期

页      面：593-600页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：the Agricultural Research and Development Promotion Center Korean Ministry of Agriculture and Forestry (ARPC Grant 204062-3) 

主　　题：abiotic stress cucumber Cucumis sativus high mobility group protein transgenic Arabidopsis plant. 

摘      要：Although high mobility group B （HMGB） proteins have been identified from a variety of plant species, their importance and functional roles in plant responses to changing environmental conditions are largely unknown. Here, we investigated the functional roles of a CsHMGB isolated from cucumber （Cucumis sativus L.） in plant responses to environmental stimuli. Under normal growth conditions or when subjected to cold stress, no differences in plant growth were found between the wild-type and transgenic Arabidopsis thaliana overexpressing CsHMGB. By contrast, the transgenic Arabidopsis plants displayed retarded germination compared with the wild-type plants when grown under high salt or dehydration stress conditions. Germination of the transgenic plants was delayed by the addition of abscisic acid （ABA）, implying that CsHMGB affects germination through an ABA-dependent way. The expression of CsHMGB had affected only the germination stage, and CsHMGB did not affect the seedling growth of the transgenic plants under the stress conditions. The transcript levels of several germination-responsive genes were modulated by the expression of CsHMGB in Arabidopsis. Taken together, these results suggest that ectopic expression of a CsHMGB in Arabidopsis modulates the expression of several germinationresponsive genes, and thereby affects the germination of Arabidopsis plants under different stress conditions.