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Dendritic cell-based immunotherapy for colon cancer using an HLA-A＊0201-restricted cytotoxic T-lymphocyte epitope from tumor-associated antigen 90K

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Cellular & Molecular Immunology 2013年第3期10卷 275-282页

作者： Ji Hee lee Myong-Suk Park Jun-Eul Hwang Sang-Hee Cho Woo-Kyun Bae Hyun-jeong Shim Dae-eun Kim Ik-Joo Chung Department of Hematology-Oncology Chonnam National University Medical School Gwangju Korea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National University Gwangiu Korea

Tumor-associated antigen 90K is implicated in cell-cell and cell-extracellular matrix adhesion through its interaction with galectin-3 and integrin-β 1 and is highly expressed in malignant tissues, making it a novel target for the development of new immunotherapies. We investigated a potential immunotherapy treatment for colon cancer using 90K-specific cytotoxic T lymphocytes induced by autologous dendritic cells and pulsed with 90K peptides. We selected three peptides （90K3s1, 90K5 and 90K523） that bind to HLA-A＊0201 molecules on the basis of their binding affinity, as determined by a peptide-T2 binding assay. Dendritic cells pulsed with 90K peptides resulted in the efficient generation of mature dendritic cells and exhibited enhanced T-cell stimulation and polarization of naive T cells toward Thl. Dendritic cells pulsed with 90K peptides generated potent cytotoxic T-lymphocytes that lysed T2 cells loaded with each 90K peptide, and 90K＋/HLA-A2＋ colon cancer cell lines, including HCT116 and SW480, in a dose-dependent and HLA-A＊O2Ol-restricted manner. No killing was observed in 90K＋/HLA-A2- DLD1 or 90K-/HLA-A2- K562 cells. Therefore, we believe that cytotoxic T-lymphocytes stimulated by 90K peptide-pulsed dendritic cells naturally recognize the 90K peptide presented by colon cancer cells in the context of HLA-A2, and kill 90K-positive tumor cells. Dendritic cells pulsed with 90K peptides led to the induction of granzyme B and perforin positive CD8＋ T cells against HCT116 and SW480 cells, but not DLD1 cells. In conclusion, 90K-specific cytotoxic T lymphocytes, generated by stimulating T cells with 90K peptide-pulsed dendritic cells, could be useful effector cells for the immunotherapv treatment of colon cancer.

关键词： 9OK colon cancer dendritic cells immunotherapy peptide

Pathological findings following radical prostatectomy in patients who are candidates for active surveillance： impact of varying PSA levels

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Asian Journal of Andrology 2011年第6期13卷 838-841页

作者： Dong Il Kang Thomas L. Jang jeongyun jeong eun Young Choi Kelly Johnson Dong Hyeon lee Wun-Jae Kim Isaac Yi Kim Section of Urologic Oncology and Dean and Betty Gallo Prostate Cancer The Cancer Institute of New Jersey Robert Wood Johnson Medical School New Brunswick NJ 08903 USA Department of Urology Inje University Medical School Busan 614-735 Korea Department of Urology Ewha Womans University Seoul 158-710 Korea Department of Urology Chungbuk National University College of Medicine Cheongju 361-711 Korea

Active surveillance is an acceptable treatment option in men with a low-risk prostate cancer. In the present study, we have retrospectively reviewed the outcomes of 509 men who fit the criteria for active surveillance but selected radical prostatectomy. Then, the impact of varying prostate-specific antigen （PSA） levels on the risk of upstaging and upgrading in these patients was assessed. Pathological characteristics of patients who fulfilled the inclusion criteria under three active surveillance criteria--those of the University of California-San Francisco, the National Cancer Institute and the European Association of Urology--were examined. The proportion of men who were deemed candidates for active surveillance but were subsequently upstaged or upgraded was determined. Of 509 patients, 186 （36.5%）, 132 （25.9%） and 88 （17.3%） men fulfilled the active surveillance criteria, respectively. Upgrading （Gleason scores 7-10） ranged from 32.8% to 38.6%, while upstaging （≥ pT3） ranged from 10.2% to 12.5%, depending on the three active surveillance criteria. After a median follow-up of 24 months, three patients developed a biochemical recurrence. When the impact of varying PSA levels was examined using a test for trend analysis in the context of PSA for each protocol, rates of upstaging were lower in men with PSA 〈4 ng m1-1. However, there was no impact of varying PSA levels on upgrading. In conclusion, commonly used active surveillance protocols carry the risks of upgrading and upstaging. More reliable and accurate markers are needed to better stratify the risks of men who are appropriate candidates for active surveillance.

关键词： prostatectomy prostate-specific antigen prostatic neoplasm risk

The 5-year functional outcomes after radical prostatectomy： a real-life experience in Korea

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Asian Journal of Andrology 2010年第6期12卷 835-840页

作者： Sung Kyu Hong Seung Hwan Doo Dae Sung Kim Won Ki lee Hong Zoo Park Ji Hyun Park Seong Jin jeong Cheol Yong Yoon Seok-Soo Byun Sang eun lee Department of Urology Seoul National University Bundang Hospital Seongnam Kyunggi-do 463-707 Korea

We investigated the functional outcomes regarding erectile function and urinary continence up to 5 years following radical prostatectomy （RP） in a cohort of Korean men. We retrospectively analyzed the clinicopathologic data of 85 Korean men who received open uni- or bilateral nerve-sparing RP for clinically localized prostate cancer and were followed up for at least 5 years postoperatively. From medical records, patients＇ status regarding urinary and erectile function at baseline and postoperative followups after RP was assessed. At 24 and 60 months after RP, proportions of subjects continent （no pads used） were 89.4% and 97.6%, respectively （P = 0.007）. Excluding subjects （n = 24） who preoperatively reported having severe erectile dysfunction or lacked relevant informations, proportions of subjects capable of having vaginal intercourse regardless of erectile aid usage were 47.5% and 37.7% at 24 and 60 months from RP, respectively （P = 0.022）. Patient＇s age at surgery （P = 0.047） and salvage radiation therapy （P = 0.026） were observed to be significant predictors of having erections sufficient for intercourse at 60 months from RP in multivariate analysis. Our results showed that while patients＇ postoperative status regarding urinary continence at 2 years from RP is generally maintained or improved at 5 year point, erectile function was observed to significantly declined from 2 years to 5 years following RP. Such decline in erectile function following RP may be more significant among men who were relatively older at surgery or those who received salvage therapy during postoperative follow-ups.

关键词： impotence prostatectomy prostatic neoplasm urinary incontinence

Prognostic role of plasma level of angiopoietin-1,angiopoietin-2,and vascular endothelial growth factor in hepatocellular carcinoma

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World Journal of Gastroenterology 2021年第27期27卷 4453-4467页

作者： Gwang Hyeon Choi eun Sun Jang Jin-Wook Kim Sook-Hyang jeong Department of Internal Medicine Seoul National University Bundang HospitalSeoul National University College of MedicineSeongnam 13620South Korea

BACKGROUND Most hepatocellular carcinomas(HCCs)are hypervascular,with characteristic features of hepatic arterial supply to the *** factors involved in tumor angiogenesis include angiopoietin-1(Ang-1),angiopoietin-2(Ang-2),and vascular endothelial growth factor(VEGF).AIM To investigate the profiles of plasma levels of angiogenesis markers in patients with HCC and evaluate their roles in predicting overall survival(OS)and progression-free survival(PFS).METHODS Plasma samples from 240 prospectively enrolled HCC patients in the very early to advanced stages were used to measure the levels of Ang-1,Ang-2,and *** associations with clinical characteristics,OS,and PFS were *** The median plasma levels of Ang-1,Ang-2,and VEGF were 3216 pg/mL,1684 pg/mL,and 26.5 pg/mL,*** plasma level of Ang-2 showed a significant increase from early stage[Barcelona clinic liver cancer(BCLC)A]to intermediate(BCLC B)and advanced stage HCC(BCLC C/D),whereas Ang-1,VEGF,and alpha-fetoprotein(AFP)levels in the plasma did not show any such *** analysis,propensity score-matched analysis,and timedependent receiver operating curve analysis revealed that Ang-2 levels had the highest predictive power for OS and *** Ang-1 nor VEGF was significantly associated with OS or *** neutrophil-to-lymphocyte ratio was an independent factor for OS and *** The plasma levels of Ang-2 correlated with liver function,tumor stage,and tumor invasiveness,showing better performance in predicting OS and PFS than AFP,Ang-1,or VEGF.

关键词： Hepatocellular carcinoma Angiogenesis Biomarker Prognosis Survival

Potential anti-tubercular agents:Hexahydro-3-phenyl indazol-2-yl(pyridin-4-yl)methanones from anti-tubercular drug isoniazid and bis(substituted-benzylidene)cycloalkanones

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Chinese Chemical Letters 2015年第5期26卷 567-571页

作者： Ayyakannu Arumugam Napoleon Fazlur-Rahman Nawaz Khan Euh Duck jeong eun Hyuk Chung Organic and Medicinal Chemistry Research Laboratory Organic Chemistry DivisionSchool of Advanced SciencesVIT-University Korea Basic Science Institute Busan Center

A series of indazol-2-yl（pyridin-4-yl）methanones, 4 were acquired from 2,6-bisbenzylidene cyclohex- anones, 3 and anti-tubercular drug （isoniazid）, and their anti-tubercular impacts were screened. Among the test compounds used against Mycobacterium tuberculosis H37 Ra cell line in the microplate alamar blue assay, the compounds 4g-j revealed moderate anti-tubercular activity with MIC 12.5 μg/mL, comparable to standard drugs （streptomycin, MIC, 6.25 μg/mL, pyrazinamide, isoniazid and ciprofloxacin with MICs of 3.125 μg/mL）.

关键词： Benzylidene cyclohexanones Hexahydro-3-phenyl indazol-2-yl (pyridin- 4-yl)methanones lsoniazid Anti-tubercular activity

Stimulation of melanogenesis by scoparone in B16 melanoma cells

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Acta Pharmacologica Sinica 2006年第11期27卷 1467-1473页

作者： jeong-yeh YANG Jeung-hyunKOO Young-gilSONG Kang-beom KWON Ju-hyung lee Hee-sook SOHN Byung-hyun PARK eun-chung JHEE Jin-woo PARK Department of Biochemistry Dental SchoolChonbuk National UniversityJeonju 561-756Korea Department of Biochemistry Medical School Chonbuk National UniversityJeonju 561-756Korea Department of Physiology School of Oriental MedicineWon-Kwang UniversityIksan 570-749Korea Department of Preventive Medicine Medical School Department of Food Science and Human Nutrition College of Home EconomyChonbuk National UniversityJeonju 561-756Korea Department of Biochemistry Medical SchoolChonbuk National UniversityJeonju 561-756Korea Institute for Healthcare Technology DevelopmentChonbuk National UniversityJeonju 561-756Korea Department of Biochemistry Medical SchoolChonbuk National UniversityJeonju 561-756Korea Institute for Healthcare Technology Development Chonbuk National UniversityJeonju 561-756Korea

Aim:The effect of coumarin derivatives on melanogenesis was investigated inB16 murine melanoma ***:Melanin content and tyrosinase activitywere analyzed *** expression of tyrosinase,tyrosinase-related protein-1(TRP-1)and tyrosinase-related protein-2(TRP-2)were measuredeither by reverse transcription-polymerase chain reaction(RT-PCR)or ***:Among the coumarin derivatives studied,scoparone(6,7-dimethoxycoumarin)was the most potent;the 6-or 7-methoxy group was found tobe essential for the stimulation of *** melanin content was greatlyincreased by scoparone in a dose-dependent manner;there was no cytotoxicity atthe effective *** increased enzyme activity as well asprotein and mRNA expression of *** addition,mRNA of TRP-1 andTRP-2 were also increased after treatment with scoparone.H-89,an inhibitor ofprotein kinase A(PKA),completely inhibited the scoparone-induced increase ofmelanogenesis and the tyrosinase ***:These results suggestthat scoparone-induced stimulation of melanogenesis is likely to occur at thetranscriptional level of melanogenesis-related enzymes through PKA signaling.

关键词： melanogenesis scoparone (6,7-dimethoxy-coumarin) tyrosinase tyrosinase-related proteins protein kinase A

Oral administration of red ginseng powder fermented with probiotic alleviates the severity of dextran-sulfate sodium-induced colitis in a mouse model

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Chinese Journal of Natural Medicines 2017年第3期15卷 192-201页

作者： JANG Sun-Hee PARK Jisang KIM Sae-Hae CHOI Kyung-Min KO eun-Sil CHA jeong-Dan lee Young-Ran JANG Hyonseok JANG Yong-Suk Department of Molecular Biology and Institute for Molecular Biology and Genetics Chonbuk National UniversityJeonju 54896Korea Department of Bioactive Material Sciences and Research Center of Bioactive Materials Chonbuk National UniversityJeonju 54896Korea Department of Efficacy Research Institute of Jinan Red GinsengJinan 55442Korea Department of Mathematics Sogang UniversitySeoul 04107Korea Department of Oral Maxillofacial Surgery Korea University Ansan HospitalAnsan 15355Korea

Red ginseng is a well-known alternative medicine with anti-inflammatory *** exerts pharmacological effects through the transformation of saponin into metabolites by intestinal *** that intestinal microflora vary among individuals,the pharmacological effects of red ginseng likely vary among *** order to produce homogeneously effective red ginseng,we prepared probiotic-fermented red ginseng and evaluated its activity using a dextran sulfate sodium(DSS)-induced colitis model in *** analysis of intestinal damage indicated that the administration of probiotic-fermented red ginseng significantly decreased the severity of colitis,compared with the control and the activity was higher than that induced by oral administration of ginseng powder or probiotics *** analysis of the levels of serum IL-6 and TNF-α,inflammatory biomarkers that are increased at the initiation stage of colitis,were significantly decreased in probiotic-fermented red ginseng-treated groups in comparison to the control *** levels of inflammatory cytokines and mR NAs for inflammatory factors in colorectal tissues were also significantly decreased in probiotic-fermented red ginseng-treated ***,oral administration of probiotic-fermented red ginseng reduced the severity of colitis in a mouse model,suggesting that it can be used as a uniformly effective red ginseng product.

关键词： Colitis Fermentation Inflammation Probiotics Red ginseng

Effect of expanding nanocellulose sponge on nasal mucosal defects in an animal model

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Regenerative Biomaterials 2020年第1期7卷 47-52页

作者： JiWon Kim Kyungbae Woo jeong Mi Kim Mi eun Choi Young-Mo Kim Su-Geun Yang Bong Sup Shim jeong-Seok Choi Department of Otorhinolaryngology Inha UniversityCollege of Medicine27 Inhang-roJung-guIncheon 22332Republic of Korea Department of Chemical Engineering Inha University100 InharoMichuholguIncheon 22212Republic of Korea Department of New Drug Development Inha UniversityCollege of MedicineB-308Chungsuk Bldg.366Seohae-DaeroJung-GuIncheon 22332Republic of Korea

Nanocellulose has emerged for a wide range of applications in biomedical engineering because of its water absorption capacity,appropriate *** investigated the hemostatic and regenerative abilities of an expanding polyvinyl alcohol(PVA)-nanocellulose sponge on nasal mucosal defects.A 3 mm-diameter nasal defect was made in experimental *** were divided into four groups with control,vaseline,PVA and PVA-nanocellulose packing *** the defect was created,bleeding times and amounts were *** materials were removed on experimental day(ED)*** ED 3,7 and 14,histological analysis and immunohistochemical study for neutrophils were *** cells were counted and epithelial thicknesses were *** amounts and times in the vaseline packing group were smaller than in the PVA ***-nanocellulose group showed less neutrophils than in the other groups on ED *** epithelium thickness in the PVA-nanocellulose group was significantly smaller than in the control group at ED 7,but at ED 14,there was no significant intergroup *** group had a significant lower inflammatory cell count than the control group on ED ***-nanocellulose sponge applied to nasal mucosal defects can significantly enhance mucosal regeneration during early wound healing.

关键词： polyvinyl alcohol nasal mucosa nanocellulose

Autoimmune hepatitis in a patient with immunoglobulin A nephropathy:A case report

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World Journal of Clinical Cases 2020年第17期8卷 3828-3834页

作者： You Hyun Jeon Da Woon Kim So jeong lee Young Joo Park Hyo Jin Kim Miyeun Han Il Young Kim Dong Won lee Sang Heon Song Soo Bong lee eun Young Seong Department of Internal Medicine Pusan National University School of MedicineBusan 49241South Korea Department of Pathology Pusan National University HospitalBusan 49241South Korea Division of Gastroenterology Department of Internal MedicineBiomedical Research InstitutePusan National University HospitalBusan 49241South Korea Division of Nephrology Department of Internal MedicineBiomedical Research InstitutePusan National University HospitalBusan 49241South Korea Division of Nephrology Department of Internal MedicinePusan National University Yangsan HospitalYangsan 626-770South Korea

BACKGROUND Immunoglobulin A nephropathy(IgAN)is the most commonly encountered glomerular disease in Asian *** has a broad clinical presentation,and it is frequently associated with other *** liver disease is well recognized as the leading cause of secondary ***,cases of IgAN associated with autoimmune hepatitis(AIH)have seldom been *** SUMMARY A 63-year-old Korean woman was admitted to Pusan National University Hospital for an evaluation of abdominal pain and elevated liver *** weeks prior,she had presented to our hospital with proteinuria of approximately 1350 mg/d and hematuria and was diagnosed with *** profiles were highly positive for antinuclear antibodies,and symptoms related to portal hypertension including ascites and peripheral edema were present.A diagnosis of AIH was made according to the simplified scoring system of the International Autoimmune Hepatitis *** immunosuppression with prednisolone and azathioprine,rapid deterioration of liver function led to end-stage liver *** a living-donor liver transplantation,liver function gradually improved,and she had maintained stable liver and kidney function at the six months *** Cases of secondary IgAN with chronic liver disease have been frequently reported in the literature but are rarely associated with *** encountered an IgAN patient with concurrent progressive liver failure due to AIH.

关键词： Immunoglobulin A nephropathy Secondary immunoglobulin A nephropathy Autoimmune hepatitis Liver transplantation Case report