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Effects of prostaglandin F_(2α) on small intestinal interstitial cells of Cajal

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World Journal of Gastroenterology 2011年第9期17卷 1143-1151页

作者： Chan Guk Park Young Dae Kim Man Yoo Kim Jae Woong Koh Jae Yeoul Jun Cheol Ho Yeum insuk so Seok Choi Department of Internal Medicine College of MedicineChosun University Department of Ophthalmology College of MedicineChosun University Department of Physiology College of MedicineChosun University Department of Physiology and Biophysics College of MedicineSeoul National University

AIM：To explore the role of prostaglandin F2α（PGF2α） on pacemaker activity in interstitial cells of Cajal（ICC）from mouse small intestine. METHODS：In this study,effects of PGF2αin the cultured ICC cells were investigated with patch clamp technology combined with Ca 2＋ image analysis. RESULTS：Externally applied PGF2α（10μmol/L）produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp *** application of flufenamic acid（a non-selective cation channel inhibitor）or niflumic acid（aCl channel inhibitor）abolished the generation of pacemaker currents but only flufenamic acid inhibited the PGF2α-induced tonic inward *** addition,the tonic inward currents induced by PGF2αwere not inhibited by intracellular application of 5’-[-thio]diphosphate trilithium *** with Ca 2＋ free solution, U-73122,an active phospholipase C inhibitor,and thapsigargin,a Ca 2＋ -ATPase inhibitor in endoplasmic reticulum,abolished the generation of pacemaker currents and suppressed the PGF2α-induced tonic inward ***,chelerythrine or calphostin C,protein kinase C inhibitors,did not block the PGF2α-induced effects on pacemaker *** recording intracellular Ca 2＋（[Ca 2＋ ]i）concentration using fluo-3/AM,PGF2α broadly increased the spontaneous[Ca 2＋ ]i oscillations. CONCLUSION：These results suggest that PGF2αcan modulate pacemaker activity of ICC by acting non-selective action channels through phospholipase C-dependent pathway via[Ca2＋]i regulation

关键词： Prostaglandin F2α Interstitial cells of Cajal Tonic inward currents Intestinal motility

Identification of TRPM7 channels in human intestinal interstitial cells of Cajal

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World Journal of Gastroenterology 2009年第46期15卷 5799-5804页

作者： Byung Joo Kim Kyu Joo Park Hyung Woo Kim Seok Choi Jae Yeoul Jun In Youb Chang Ju-Hong Jeon insuk so Seon Jeong Kim School of Korean Medicine Pusan National University Department of Surgery College of MedicineSeoul National University Department of Physiology College of MedicineChosun University Department of Anatomy College of MedicineChosun University Center for Bio-Artif icial Muscle and Department of Physiology College of MedicineSeoul National University Center for Bio-Artifi cial Muscle and Depart-ment of Biomedical Engineering Hanyang University

AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.

关键词： Electrophysiology Interstitial cells of Cajal TRPM cation channels Transient receptor potential melastatin-type 7 protein Human Gastrointestinal tract

Inhibition of pacemaker activity in interstitial cells of Cajal by LPS via NF-κB and MAP kinase

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World Journal of Gastroenterology 2013年第8期19卷 1210-1218页

作者： Dong Chuan Zuo Seok Choi Pawan Kumar Shahi Man Yoo Kim Chan Guk Park Young Dae Kim Jun Lee In Yeoup Chang insuk so Jae Yeoul Jun Department of Physiology College of MedicineChosun UniversityGwangju 501-759South Korea Department of Internal Medicine College of MedicineChosun UniversityGwangju 501-759South Korea Department of Anatomy College of MedicineChosun UniversityGwangju 501-759South Korea Department of Physiology and Biophysics College of MedicineSeoul National UniversitySeoul 56-1South Korea

AIM:To investigate lipopolysaccharide(LPS) related signal transduction in interstitial cells of Cajal(ICCs) from mouse small ***:For this study,primary culture of ICCs was prepared from the small intestine of the *** was treated to the cells prior to measurement of the membrane currents by using whole-cell patch clamp *** was used to examine the expression of the proteins in ***:LPS suppressed the pacemaker currents of ICCs and this could be blocked by AH6809,a prostaglandin E2-EP2 receptor antagonist or NG-Nitro-Larginine Methyl Ester,an inhibitor of nitric oxide(NO) ***-like receptor 4,inducible NO synthase or cyclooxygenase-2 immunoreactivity by specific antibodies was detected on ***(antioxidant agent) had no action on LPS-induced action in *** actions were blocked by nuclear factor kB(NF-kB) inhibitor,actinomycin D(a gene transcription inhibitor),PD 98059(a p42/44 mitogen-activated protein kinases inhibitor) or SB 203580 [a p38 mitogen-activated protein kinases(MAPK) inhibitor].SB 203580 also blocked the prostaglandin E2-induced action on pacemaker currents in ICCs but not ***:LPS inhibit the pacemaker currents in ICCs via prostaglandin E2-and NO-dependent mechanism through toll-like receptor 4 and suggest that MAPK and NF-kB are implicated in these actions.

关键词： Interstitial cells of Cajal Lipopolysaccharide Mitogen-activated protein kinases Nuclear factor kB Small intestine

WheatNet： a Genome-Scale Functional Network for Hexaploid Bread Wheat, Triticum aestivum

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Molecular Plant 2017年第8期10卷 1133-1136页

作者： Tak Lee sohyun Hwang Chan Yeong Kim Hongseok Shim Hyojin Kim Pamela C. Ronald EdwardM. Marcotte insuk Lee Department of Biotechnology College of Life Science and Biotechnology Yonsei University Seou1120-749 Korea Center for Systems and Synthetic Biology Institute for Cellular and Molecular Biology University of Texas at Austin Austin TX 78712 USA Department of Biomedical Science College of Life Science CHA University Seongnam-si 13496 Korea Department of Plant Pathology University of California Davis CA 95616 USA Joint Bioenergy Institute Emeryville CA 94608 USA The Genome Center University of California Davis CA 95616 USA Department of Molecular Bioseiences University of Texas at Austin Austin TX 78712 USA

Dear Editor Gene networks provide a system-level overview of genetic organizations and enable the dissection of functional modules underlying complex traits. Integration of diverse genomics data based on the Bayesian statistics framework has been successfully applied to the construction of genome-scale functional networks for major crop species such as rice （Lee et al., 2011）, soybean （Kim et al., 2017）, and tomato （Kim et al.,

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LSA Based Classification of Advertising Spam Reviews

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Computer Technology and Application 2011年第12期2卷 998-1006页

作者： insuk Park Hanhoon Kang Seong Joon Yoo Department of Computer Engineering Sejong University 98 Gunja Gwangfin Seoul 143-747 Korea

In this study, methods to classify advertising reviews from shopping mall reviews are suggested. Advertising reviews are mostly written by companies and contain advertising contents. There are a few studies regarding the classification of opinion spam documents, which is very rare in foreign studies; however, there are no studies that classify advertising reviews from Korean reviews. In this study, the Naive Bayes Classifier was used to classify review documents and the POS （Part-of-Speech）-Tagging and bigram methods were used to extract specific words. The frequency calculation methods for the probability value of specific words were：（1） The general number of appearances of words （2） the frequency calculation of specific words through the suggested Latent Semantic Analysis （LSA）, and by recalculating the result from （1） in （2）, the performances of each method were compared. As a result, the methods from （2） showed 88.43% accuracy which is 8.89% higher than 79.54% which was the previous result from using the POS-Tagging ＋ Bigram method. Therefore, it was proved that the method suggested in this study is effective at classifying or extracting advertising reviews from Korean product review documents.

关键词： Opinion review spam review advertising review latent semantic analysis (LSA).

TomatoNet A Genome-wide Co-functional Network for Unveiling Complex Traits of Tomato, a Model Crop for Fleshy Fruits

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Molecular Plant 2017年第4期10卷 652-655页

作者： Hyojin Kim Bong Suk Kim Jung Eun Shim sohyun Hwang Sunmo Yang Eiru Kim Aniafi S. Iver-Pascuzzi2 insuk Lee Department of Biotechnology College of Life Science and Biotechnology Yonsei University Seou1120-749 Korea Department of Botany and Plant Pathology Purdue University West Lafayette IN 47907 USA

Tomato （solanum lycopersicum） is the second most important vegetable crop globally, with a total world production valued at over $74 billion （FAOSTAT Statistics Database, 2009）. Tomato is also an important model c... 详细信息

关键词： Genome-wide Co-functional Network Unveiling Complex Traits Tomato