Identification of TRPM7 channels in human intestinal interstitial cells of Cajal

作     者：Byung Joo Kim Kyu Joo Park Hyung Woo Kim Seok Choi Jae Yeoul Jun In Youb Chang Ju-Hong Jeon Insuk So Seon Jeong Kim 

作者机构：School of Korean MedicinePusan National University Department of SurgeryCollege of MedicineSeoul National University Department of PhysiologyCollege of MedicineChosun University Department of AnatomyCollege of MedicineChosun University Center for Bio-Artif icial Muscle and Department of PhysiologyCollege of MedicineSeoul National University Center for Bio-Artifi cial Muscle and Depart-ment of Biomedical EngineeringHanyang University 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2009年第15卷第46期

页      面：5799-5804页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：Supported by The Creative Research Initiative Center for Bio-Artificial Muscle of the Ministry of Education Science and Technology (MEST) in Korea 

主　　题：Electrophysiology Interstitial cells of Cajal TRPM cation channels Transient receptor potential melastatin-type 7 protein Human Gastrointestinal tract 

摘      要：AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.