Effects of prostaglandin F_(2α) on small intestinal interstitial cells of Cajal
Effects of prostaglandin F_(2α) on small intestinal interstitial cells of Cajal作者机构：Department of Internal MedicineCollege of MedicineChosun University Department of OphthalmologyCollege of MedicineChosun University Department of PhysiologyCollege of MedicineChosun University Department of Physiology and BiophysicsCollege of MedicineSeoul National University
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第9期
页 面：1143-1151页
核心收录：
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金：Supported by Research Fund from Chosun Hospital 2008
主 题：Prostaglandin F2α Interstitial cells of Cajal Tonic inward currents Intestinal motility
摘 要：AIM：To explore the role of prostaglandin F2α（PGF2α） on pacemaker activity in interstitial cells of Cajal（ICC）from mouse small intestine. METHODS：In this study,effects of PGF2αin the cultured ICC cells were investigated with patch clamp technology combined with Ca 2＋ image analysis. RESULTS：Externally applied PGF2α（10μmol/L）produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp *** application of flufenamic acid（a non-selective cation channel inhibitor）or niflumic acid（aCl channel inhibitor）abolished the generation of pacemaker currents but only flufenamic acid inhibited the PGF2α-induced tonic inward *** addition,the tonic inward currents induced by PGF2αwere not inhibited by intracellular application of 5’-[-thio]diphosphate trilithium *** with Ca 2＋ free solution, U-73122,an active phospholipase C inhibitor,and thapsigargin,a Ca 2＋ -ATPase inhibitor in endoplasmic reticulum,abolished the generation of pacemaker currents and suppressed the PGF2α-induced tonic inward ***,chelerythrine or calphostin C,protein kinase C inhibitors,did not block the PGF2α-induced effects on pacemaker *** recording intracellular Ca 2＋ （[Ca 2＋ ]i）concentration using fluo-3/AM,PGF2α broadly increased the spontaneous[Ca 2＋ ]i oscillations. CONCLUSION：These results suggest that PGF2αcan modulate pacemaker activity of ICC by acting non-selective action channels through phospholipase C-dependent pathway via[Ca2＋]i regulation
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