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复合抗氧化剂处理对五香牛肉低温贮藏品质的影响

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食品工业科技 2021年第11期42卷 204-210页

作者：陈洪生国慧刁静静刘欢张根生黑龙江八一农垦大学食品学院黑龙江大庆163319 黑龙江八一农垦大学国家杂粮工程技术研究中心黑龙江大庆163319 哈尔滨商业大学食品工程学院黑龙江哈尔滨150076

为改善五香牛肉在低温贮藏期间的安全性,降低合成抗氧化剂的使用量,筛选一种可以改善五香牛肉贮藏品质的天然保鲜剂。本文以五香牛肉为试验材料,采用无添加抗氧化剂组作为第一组(对照组),另外分别设置四种复合天然抗氧化剂处理组,即0.05... 详细信息

为改善五香牛肉在低温贮藏期间的安全性,降低合成抗氧化剂的使用量,筛选一种可以改善五香牛肉贮藏品质的天然保鲜剂。本文以五香牛肉为试验材料,采用无添加抗氧化剂组作为第一组(对照组),另外分别设置四种复合天然抗氧化剂处理组,即0.05%乳酸链球菌素+0.05%丁香+0.12%肉桂为第二组,0.05%乳酸链球菌素+0.05%丁香+0.03%迷迭香为第三组,0.05%丁香+0.03%迷迭香+0.12%肉桂为第四组,0.05%乳酸链球菌素+0.05%丁香+0.03%迷迭香+0.12%肉桂为第五组,通过分析五香牛肉在贮藏期间微生物指标、理化指标和感官指标的变化,探讨复合天然抗氧化剂对五香牛肉贮藏保鲜效果的影响。结果表明,在低温贮藏条件下,与对照组相比,第五组可以显著减缓五香牛肉的氧化损伤,延缓产品品质降低(P<0.05),且具有较好的感官品质。60 d时,第五组的TBARS值为(0.30±0.01)mg/kg,挥发性盐基氮值为(12.08±0.21)mg/100 g,菌落总数为(3.81±0.04)lg(CFU/g),显著优于对照组(P<0.05);感官评定分析结果也同样得出:60 d时第五组的总体可接受性显著高于对照组(P<0.05)。综合试验结果得出,0.05%乳酸链球菌素+0.05%丁香+0.03%迷迭香+0.12%肉桂处理组对低温贮藏五香牛肉的保鲜效果最好。

关键词：五香牛肉理化指标天然抗氧化剂 N-二甲基亚硝胺

Clinical characteristics and prognosis analysis in septic patients with a high level of serum procalcitonin

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Clinical characteristics and prognosis analysis in septic pa...

第四届重症心脏全国学术大会暨第五届西湖重症医学大会、2019年浙江省重症医学学术大会（重症心脏-心与心外器官)

作者： Chuli Pan Feifei Zhou Xiuhui Lin Dongying Wang Nanxia Xuan Baoping Tian Junwei Tu Wei Cui gensheng zhang

Background:Although the application of serum procalcitonin(PCT) in guiding the diagnosis and treatment of sepsis becomes more and more widely,its significance in a subgroup of septic patients with a high level of serum PCT(>100μg/L) remains largely ***,we investigated the clinical characteristics and prognosis of such ***:A retrospective study for septic patients with a high level of serum PCT within 48 hours of hospitalization was conducted in our hospital from February 2013 to December *** characteristics including the demographics,main laboratory examinations and outcomes were extracted from the medical *** patients were divided into survivors and non-survivors based on 28-day *** differences of these variables were compared between groups of survivors and non-survivors.A multi-factorial logistic regression analysis was used for the evaluation of independent risk factors of ***:149 cases of such septic patients were *** were 98 males and 51 females with an average age of 63(52,77) years.98(65.8%) patients were treated in intensive care unit(ICU),and 51(34.2%) patients were treated in general *** total mortality rate was 32.2%(48/149).The median acute physiology and chronic health evaluation II(APACHE II) score was 15(11,19),and the median sequential organ failure assessment(SOFA) score was 10(7,14).The APACHE II [20(18,27) vs.14(10,16),P<0.001]and SOFA scores [14(12,18) vs.8(6,11),P<0.001],and the incidence of complications including multiple organ dysfunction syndrome(MODS)(87.5% vs.37.6%,P<0.001),septic shock(100% vs.60.4%,P<0.001) and respiratory failure(72.9% vs.38.6%,P<0.001) were significantly higher in non-survivors than in *** addition,some laboratory indexes including liver dysfunction,coagulation dysfunction,levels of myocardial enzymes and lactic acid were higher in non-survivors than in survivors(all P<0.05),whereas the platelet(PLT) count was significantly lowe

关键词： High level of serum PCT(>100μg/L) Sepsis Clinical characteristics Prognostic factors

Clinical characteristics and prognosis analysis in septic patients with a high level of serum procalcitonin

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Clinical characteristics and prognosis analysis in septic pa...

2019年浙江省医学会医学微生物与免疫学分会学术大会

作者： Chuli Pan Feifei Zhou Xiuhui Lin Dongying Wang Nanxia Xuan Baoping Tian Junwei Tu Wei Cui gensheng zhang Department of Respiratory Medicine Jinhua Municipal Central Hospital Department of Critical Care Medicine Second Affiliated HospitalZhejiang University School of Medicine Department of Critical Care Medicine Ningbo City Medical Treatment Center Lihuili Hospital Department of Respiratory Medicine Second Affiliated HospitalWenzhou Medical University

Background:Although the application of serum procalcitonin(PCT) in guiding the diagnosis and treatment of sepsis becomes more and more widely,its significance in a subgroup of septic patients with a high level of serum PCT(>100 μg/L) remains largely ***,we investigated the clinical characteristics and prognosis of such ***:A retrospective study for septic patients with a high level of serum PCT within 48 hours of hospitalization was conducted in our hospital from February 2013 to December *** characteristics including the demographics,main laboratory examinations and outcomes were extracted from the medical *** patients were divided into survivors and non-survivors based on 28-day *** differences of these variables were compared between groups of survivors and non-survivors.A multi-factorial logistic regression analysis was used for the evaluation of independent risk factors of ***:149 cases of such septic patients were *** were 98 males and 51 females with an average age of 63(52,77) years.98(65.8%) patients were treated in intensive care unit(ICU),and 51(34.2%) patients were treated in general *** total mortality rate was 32.2%(48/149).The median acute physiology and chronic health evaluation II(APACHE II) score was 15(11,19),and the median sequential organ failure assessment(SOFA)score was 10(7,14).The APACHE II [20(18,27) vs.14(10,16),P<0.001]and SOFA scores [14(12,18) vs.8(6,11),P<0.001],and the incidence of complications including multiple organ dysfunction syndrome(MODS)(87.5%vs.37.6%,P<0.001),septic shock(100% vs.60.4%,P<0.001) and respiratory failure(72.9% vs.38.6%,P<0.001)were significantly higher in non-survivors than in *** addition,some laboratory indexes including liver dysfunction,coagulation dysfunction,levels of myocardial enzymes and lactic acid were higher in non-survivors than in survivors(all P<0.05),whereas the platelet(PLT) count was significantly lower

关键词： High level of serum PCT(>100μg/L) Sepsis Clinical characteristics Prognostic factors

Clinical characteristics and risk factors in mixed-enterococcal bloodstream infections

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Clinical characteristics and risk factors in mixed-enterococ...

第四届重症心脏全国学术大会暨第五届西湖重症医学大会、2019年浙江省重症医学学术大会（重症心脏-心与心外器官)

作者： Cheng Zheng Jiachang Cai Haizhou Liu Shufang zhang Li Zhong Nanxia Xuan Hongwei Zhou Kai zhang Yesong Wang Xijiang zhang Baoping Tian Zhaocai zhang Wei Cui gensheng zhang

Objectives:Although the enterococcal bloodstream infections(EBSI) are often observed in clinic,the mixedEBSI were few *** aim of this study was to investigate the clinical characteristics and risk factors of mixedEBSI in comparison with monomicrobial EBSI(mono-EBSI).Methods:A single center retrospective observational study was performed between Jan 1,2013 and Dec 31,2018 in a tertiary *** patients with EBSI were enrolled,and their data were collected by reviewing electronic medical ***:A total of 451 patients with EBSI were enrolled including 157 cases(34.8%) with *** most common co-pathogens were Gram-negative bacteria(57.1%),followed by Gram-positive bacteria(38.3%) and fungi(4.6%).In multivariable analysis,burn injury(adjusted odds ratio [OR],0.136;95% confidence interval [CI],0.050-0.373),and length of prior hospital stay(aOR,1.009;95% CI,1.001-1.017) were associated with *** with mixed-EBSI developed with more proportion of septic shock(19% vs.31.8%,p=0.002),prolonged length of ICU stay [9(0,25) vs.15(2.5,36),p<0.001] and hospital stay [29(16,49) vs.33(18.5,63),p=0.031].The mortality was not significantly different between mixed-EBSI and mono-EBSI(p=0.219).Conclusions:A high rate of mixed-EBSI was among EBSI,and the Gram-negative bacteria were the predominant co-existed *** injury and length of prior hospital stay were independent risk factors for *** the mortality was not different,patients with mixed-EBSI had poor outcomes in comparison with mono-EBSI,which merits more attention by physicians in the future.

关键词： Bloodstream infections Mixed-enterococcal bloodstream infections Monomicrobial enterococcal bloodstream infections Clinical characteristics Risk factors

Clinical characteristics and risk factors in mixed-enterococcal bloodstream infections

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Clinical characteristics and risk factors in mixed-enterococ...

2019年浙江省医学会医学微生物与免疫学分会学术大会

作者： Cheng Zheng Jiachang Cai Haizhou Liu Shufang zhang Li Zhong Nanxia Xuan Hongwei Zhou Kai zhang Yesong Wang Xijiang zhang Baoping Tian Zhaocai zhang Changming Wang Wei Cui gensheng zhang Department of Critical Care Medicine Second Affiliated HospitalZhejiang University School of Medicine Department of Critical Care Medicine Taizhou Municipal Hospital Clinical Microbiology Laboratory Second Affiliated HospitalZhejiang University School of Medicine Department of Critical Care Medicine Zhejiang Rehabilitation Hospital Department of Cardiology Second Affiliated HospitalZhejiang University School of Medicine Department of Critical Care Medicine Huzhou First People's Hospital

Objectives:Although the enterococcal bloodstream infections(EBSI) are often observed in clinic,the mixedEBSI were few *** aim of this study was to investigate the clinical characteristics and risk factors of mixedEBSI in comparison with monomicrobial EBSI(mono-EBSI).Methods:A single center retrospective observational study was performed between Jan 1,2013 and Dec 31,2018 in a tertiary *** patients with EBSI were enrolled,and their data were collected by reviewing electronic medical ***:A total of 451 patients with EBSI were enrolled including 157 cases(34.8%) with *** most common co-pathogens were Gram-negative bacteria(57.1%),followed by Gram-positive bacteria(38.3%) and fungi(4.6%).In multivariable analysis,burn injury(adjusted odds ratio [OR],0.136;95% confidence interval [CI],0.050-0.373),and length of prior hospital stay(aOR,1.009;95% CI,1.001-1.017) were associated with *** with mixed-EBSI developed with more proportion of septic shock(19% vs.31.8%,p=0.002),prolonged length of intensive care unit(ICU) stay [9(0,25) vs.15(2.5,36),p<0.001] and hospital stay [29(16,49) vs.33(18.5,63),p=0.031].The mortality was not significantly different between mixed-EBSI and mono-EBSI(p=0.219).Conclusions:A high rate of mixed-EBSI was among EBSI,and the Gram-negative bacteria were the predominant co-existed *** injury and length of prior hospital stay were independent risk factors for *** the mortality was not different,patients with mixed-EBSI had poor outcomes in comparison with mono-EBSI,which merits more attention by physicians in the future.

关键词： Bloodstream infections Mixed-enterococcal bloodstream infections Monomicrobial enterococcal bloodstream infections Clinical characteristics Risk factors

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低脂和低盐萨拉米发酵香肠的研究进展

肉类研究 2020年第9期34卷 68-73页

作者：韩冰郑野张天琪张根生哈尔滨商业大学食品工程学院黑龙江哈尔滨150076

萨拉米发酵香肠具有营养价值高及风味独特等优点,深受消费者喜爱。与大部分肉制品相比,传统萨拉米发酵香肠中食盐(约8%)和脂肪(约32%)含量较高,长期食用高脂高盐食品可能会诱发心脑血管疾病,因此,降低萨拉米发酵香肠中食盐和脂肪添加量... 详细信息

萨拉米发酵香肠具有营养价值高及风味独特等优点,深受消费者喜爱。与大部分肉制品相比,传统萨拉米发酵香肠中食盐(约8%)和脂肪(约32%)含量较高,长期食用高脂高盐食品可能会诱发心脑血管疾病,因此,降低萨拉米发酵香肠中食盐和脂肪添加量势在必行。本文综述了近年来萨拉米发酵香肠的降脂、降盐方法,介绍了非热加工技术在低脂和低盐萨拉米发酵香肠中的应用,为优化低脂和低盐萨拉米发酵香肠提供理论参考。

关键词：低脂低盐萨拉米发酵香肠品质非热加工技术研究进展

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中西医结合治疗气虚血瘀型冠心病50例

光明中医 2016年第16期31卷 2402-2403页

作者：张根生河南省周口市中医院心内科周口466000

目的探析参芪血府逐瘀汤合西药对气虚血瘀型冠心病的治疗效果。方法选取2013年2月—2014年6月气虚血瘀型冠心病患者100例为研究对象,分为观察组与对照组各50例,其中予以对照组西药常规治疗方案,观察组在对照组的基础上配合口服参芪血府... 详细信息

目的探析参芪血府逐瘀汤合西药对气虚血瘀型冠心病的治疗效果。方法选取2013年2月—2014年6月气虚血瘀型冠心病患者100例为研究对象,分为观察组与对照组各50例,其中予以对照组西药常规治疗方案,观察组在对照组的基础上配合口服参芪血府逐瘀汤治疗,一剂/天,早晚各服用一次,连续服用一个月,观察比较两组患者相关指标改变情况以及病情好转情况。结果观察组治疗心绞痛的总有效率达96.0%,优胜于对照组(76.0%),差异显著有意义(P<0.05);观察组血脂、血液流变学改善情况远远优胜于对照组,差异显著有意义(P<0.05)。结论参芪血府逐瘀汤治疗冠心病具有卓越成效,是值得推广应用的中医疗法。

关键词：冠心病参芪血府逐瘀汤气虚血瘀型

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护国运动前夕日本对华政策的变化原因

大理学院学报（综合版） 2013年第1期12卷 48-51页

作者：张根生云南中医学院思想政治理论课教学研究部昆明650500

"一战"的爆发为日本侵华势力提供了良机,不过在对华问题上日本国内朝野的意见并不一致,尤其是在对待袁世凯的问题上矛盾更为尖锐。"倒袁"派一度占到上风,致使北洋政府被迫接受"二十一条",然而出乎日本军国主义者意料的是中国人民爆发了... 详细信息

"一战"的爆发为日本侵华势力提供了良机,不过在对华问题上日本国内朝野的意见并不一致,尤其是在对待袁世凯的问题上矛盾更为尖锐。"倒袁"派一度占到上风,致使北洋政府被迫接受"二十一条",然而出乎日本军国主义者意料的是中国人民爆发了声势浩大的反日运动,在一定程度上促使日本政府被迫采取温和的对华政策,即从"迫袁附日"到"亲善提携"。

关键词：元老派少壮派迫袁附日亲善提携

生长素响应因子GmARF10正调节大豆叶片的衰老进程

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西北植物学报 2014年第9期34卷 1749-1756页

作者：李小平曾庆发张根生赵娟淮北师范大学生命科学学院安徽省资源植物重点实验室安徽淮北235000

采用实时荧光定量PCR(qPCR)分析了GmARF10表达模式。通过挖掘大豆基因组信息并参考拟南芥同源基因序列,分析了大豆生长素响应因子GmARF10和小RNA分子Gm-miR160作用位点,构建了pGmARF10∷mGmARF10(mGmARF10)抗降解表达载体;利用农杆菌介... 详细信息

采用实时荧光定量PCR(qPCR)分析了GmARF10表达模式。通过挖掘大豆基因组信息并参考拟南芥同源基因序列,分析了大豆生长素响应因子GmARF10和小RNA分子Gm-miR160作用位点,构建了pGmARF10∷mGmARF10(mGmARF10)抗降解表达载体;利用农杆菌介导法转化大豆,并对转基因植株叶片发育表型进行了分析。结果表明:GmARF10在大豆[Glycine max(L.)Merri.]根、茎、叶、花和果荚中均有一定程度的表达,其中花内表达量最高,茎内最低,第一复叶表达量低于子叶和第一对真叶。mGmARF10转基因植株复叶形状和大小与对照组没有明显的差异,但其叶绿素含量和最大光量子效率(Fv/Fm)明显下降,叶片衰老标记基因GmCYSP1表达显著增加。研究认为,大豆生长素响应因子GmARF10参与了叶片衰老进程并可能是叶片衰老信号的重要组份。

关键词：生长素响应因子小RNA 抗降解表达叶片衰老