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Two Cases of Multi-antibiotic Resistant Cronobacter spp. Infections of Infants in China＊

Biomedical and Environmental Sciences 2017年第8期30卷 601-605页

作者： cui Jing Hua YU Bo XIANG Yun ZHANG Zhen ZHANG Ting ZENG Ying Chun cui zhi gang HUO Xi Xiang State Key Laboratory for infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Hubei provincial center for disease control and prevention Wuhan 430079 Hubei China Department of Laboratory Medicine Wuhan Medical and Health Center for Women and Children Tongji Medical College Huazhong University of Science and Technology Wuhan 430015 Hubei China

Infections by Cronobacter spp. are hazardous to infants since they can lead to neonatal meningitis, bacteremia, and necrotizing enterocolitis. Cronobacter spp. are frequently resistant to 13-1actam derivatives, macrolides, and aminoglycosides. In addition, multi-resistant strains have also been detected. In China, the isolation rate of Cronobacter spp. from commercial powdered infant formula （PIF） or follow-up formula （FUF） is relatively high. Nevertheless, clinical cases of Cronobacter infection have been ignored to date. Here we describe two cases of Cronobacter infection detected at the Wuhan Women and Children Medical Care Center Hospital （Wuhan City, China）. We provide the genomic analysis of the isolates and the antibiotic-resistance profiles of the two strains. The Cronobacter strains identified in this study were not susceptible to third-generation cephalosporins, aminoglycoside, and/or trimethoprim-sulfamethoxazole. Whole genome sequencing revealed various genes known to encode antibiotic resistance. Future studies are needed to determine whether the genes predicted in this study are functional. As with Enterobocter spp., the antibiotic resistance of Cronobocter is a serious issue that requires more attention.

关键词： Multi-antibiotic resistant Cronobacter spp. Infant Case report

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Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Aeromonas Isolates from Clinical Patients, Tap Water Systems, and Food

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Biomedical and Environmental Sciences 2020年第6期33卷 385-395页

作者： MENG Shuang WANG Yong Lu LIU Chen Geng YANG Jing YUAN Min BAI Xiang Ning JIN Dong LIANG Jun Rong cui zhi gang LI Juan State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 10026China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang 310058HangzhouChina Ma'anshan Center for Disease Control and Prevention Ma'anshan 243031AnhuiChina Clinical Laboratoty of Xuanwu Hospital Captital Medcial University'Beijing 100053China

Objective This study aimed to evaluate the genetic diversity,virulence,and antimicrobial resistance of Aeromonas isolates from clinical patients,tap water systems,and *** Ninety Aeromonas isolates were obtained from Ma’anshan,Anhui province,China,and subjected to multi-locus sequence typing(MLST)with six housekeeping *** taxonomy was investigated using concatenated gyr B-cpn60 sequences,while their resistance to 12 antibiotics was *** putative virulence factors and several resistance genes were identified by PCR and *** The 90 Aeromonas isolates were divided into 84 sequence types,80 of which were novel,indicating high genetic *** Aeromonas isolates were classified into eight different *** assays identified virulence genes in the isolates,with the enterotoxin and hemolysin genes act,aer A,alt,and ast found in 47(52.2%),13(14.4%),22(24.4%),and 12(13.3%)of the isolates,*** majority of the isolates(≥90%)were susceptible to aztreonam,imipenem,cefepime,chloramphenicol,gentamicin,tetracycline,and ***,several resistance genes were detected in the isolates,as well as a new mcr-3 *** Sequence type,virulence properties,and antibiotic resistance vary in Aeromonas isolates from clinical patients,tap water systems,and food.

关键词： Aeromonas Multi-locus sequence typing Multidrug resistance Virulence gene Antimicrobial resistance gene

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Comparative Study of the Genetic Diversity, Antimicrobial Resistance, and Pathogenicity of Aeromonas Isolates from Clinical Patients and Healthy Individuals

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Biomedical and Environmental Sciences 2021年第6期34卷 454-464页

作者： MENG Shuang DU Xiao Li WANG Yong Lu QU Feng Tian XIE Gui Lin ZHOU Hai Jian HU Jin Rui QIN Zheng WANG Yue KAN Biao cui zhi gang State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Ma'anshan Center for Disease Control and Prevention Ma'anshan 243000AnhuiChina College of Life Science Northeast Agricultural UniversityHa'erbin 150000HeilongjiangChina School of Public Health Jiamusi UniversityJiamusi 154000HeilongjiangChina

Objective This study was performed to compare the genetic diversity,virulence,and antimicrobial resistance of Aeromonas strains isolated from patients and healthy *** A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma’anshan City,Anhui *** taxonomy was investigated using concatenated gyrB-cpn60 sequences,and their resistance to 12 antibiotics was *** pathogenicity of these strains was examined through beta-hemolysis,protease activity,and virulence gene *** The 57 Aeromonas strains were divided into 55 sequence *** these types,21 were novel,suggesting that their genetic diversity was *** Aeromonas isolates could be divided into 7 species,and the positive rates of beta-hemolysis and protease activity were 49.1%and 73.7%,*** detection rate of clinical patients in terms of beta-hemolysis and protease activity was higher than that of healthy *** the four most common Aeromonas strains,*** had the highest detection rate of virulence *** multidrug resistance rate of the clinical isolates was much higher than that of the strains isolated from healthy *** The taxonomy,virulence properties,and antibiotic resistance of Aeromonas isolates from patients differ from those of the isolates from healthy individuals.

关键词： Aeromonas Genetic diversity Multidrug resistance Virulence gene Antimicrobial resistance gene

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Genetic Diversity,Antibiotic Resistance,and Pathogenicity of Aeromonas Species from Food Products in Shanghai,China

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Biomedical and Environmental Sciences 2022年第9期35卷 842-853页

作者： QU Feng Tian WANG Wen Qing LIU Qian ZHOU Hai Jian HU Jin Rui DU Xiao Li WANG Yue XUE Jia Qi cui zhi gang XIE Gui Lin MENG Shuang College of Life Science Northeast Agricultural UniversityHa’erbin 150000HeilongjiangChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Shanghai Pudong New Area Center for Disease Control and Prevention Shanghai 200136China Laboratory Medicine Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Clinic Medical College North China University of Science and TechnologyTangshan 063000HebeiChina

Objective Aeromonas has recently been recognized as an emerging human *** diarrhea is a phenomenon occurring *** study was designed to determine the prevalence,genetic diversity,antibiotic resistance,and pathogenicity of Aeromonas strains isolated from food products in *** Aeromonas isolates(n=79)collected from food samples were analyzed using concatenated gyrB-cpn60 *** antibiotic resistance of these isolates was determined using antimicrobial susceptibility *** was assessed usingβ-hemolytic,extracellular protease,virulence gene detection,*** liquid toxicity(LT),and cytotoxicity *** Eight different species were identified among the 79 *** most prevalent Aeromonas species were ***[62(78.5%)],***[6(7.6%)],***[3(3.8%)],and ***[3(3.8%)].The Aeromonas isolates were divided into 73 sequence types(STs),of which 65 were *** isolates were hemolytic(45.6%)and protease-positive(81.0%).The most prevalent virulence genes were act(73.4%),fla(69.6%),aexT(36.7%),and ascV(30.4%).The results of *** LT and cytotoxicity assays revealed that *** and *** were more virulent than ***,***,and *** resistance genes[tetE,blaTEM,tetA,qnrS,aac(6)-Ib,mcr-1,and mcr-3]were detected in the *** multidrug-resistance rate of the Aeromonas isolates was 11.4%,and 93.7%of the Aeromonas isolates were resistant to *** The taxonomy,antibiotic resistance,and pathogenicity of different Aeromonas species *** Aeromonas isolates *** and *** were highly pathogenic,indicating that food-derived Aeromonas isolates are potential risks for public health and food *** monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China.

关键词： Aeromonas Genetic diversity Antibiotic resistance Virulence gene Cytotoxicity assay Multidrug-resistance

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Enzyme-Linked Immunosorbent Assay for Detecting Syphilis Dry Blood Spot Samples

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Chinese Journal of Biomedical Engineering(English Edition) 2022年第2期31卷 64-71页

作者： cui zhi-gang DU Yan-li JIANG Yu-shan ZHENG Lu Department of Clinical Laboratory Harbin Blood CenterHarbin Heilongjiang 150056China

Objective:To explore the feasibility of enzyme-linked immunosorbent assay(ELISA)in detecting syphilis dry blood ***:Based on dry blood spot samples,laboratory linear dial,laboratory basic dial,laboratory interference dial,and laboratory precision dial were *** linear range,sensitivity,specificity,precision and other performances of ELISA for detecting syphilis dry blood spot samples were comprehensively evaluated,and the stability of dry blood spot samples at 37℃was *** addition,112 suspected syphilis antibody-positive plasma samples were selected as the control,and dry blood spot samples were prepared *** clinical sensitivity,clinical specificity,positive predictive value and negative predictive value of dry blood spot samples and plasma samples in ELISA for syphilis detection were compared,and the consistency and correlation between the two samples were analyzed by the Kappa consistency test and Spearman rank correlation ***:The results of the linear analysis showed that the serial dilution of dry blood spot samples in ELISA for syphilis antibody ranged from 23to 27,and there was a good linear range[R2=0.9862(P<0.05)].Sensitivity and specificity analysis showed that the positive coincidence rate and negative coincidence rate of the two detection methods were 100%(15/15).The results of the interference dial test showed that ELISA based on dry blood spot samples could accurately detect 6 syphilis antibody samples from 18samples,and the detection accuracy rate was 100.00%(6/6).The results of the precision test showed that the RSD of syphilis antibody detection in dry blood spot samples with different dilution times(23,25and 27)was 0.24%to 3.87%between spots,0.06%to4.07%between batches and 0.49%to 3.88%between *** 7 days,the inter-day RSD of dry blood spots with different dilution times(23,25,27)were 0.27%,0.65%and 0.95%,*** clinical sensitivity,clinical specificity,positive predictive value a

关键词： syphilis blood spot sample enzyme-linked immunosorbent assay

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Structural Variation of the Superintegron in the Toxigenic Vibrio cholerae O1 El Tor

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Biomedical and Environmental Sciences 2011年第6期24卷 579-592页

作者： GAO Yan PANG Bo WANG Hai Yin ZHOU Hai Jian cui zhi gang KAN Biao State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable DiseaseControl and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To understand the genetic structures and variations of the superintegron （Sl） in Vibrio cholerae isolated in the seventh cholera pandemic. Methods Polymerase chain reaction scanning and fragment sequencing were used. Sixty toxigenic V. cholerae O1 El Tor strains isolated between 1961 and 2008 were analyzed. Results Some variations were found, including insertions, replacements, and deletions. Most of the deletions were probably the result of recombination between V. cholerae repeat sequences. The majority of the variations clustered together. The Sis of the strains isolated in the 1960s and 1970s showed more diversity, whereas SI cassette variations in strains isolated in the 1990s and after were lower, with -24 kb signature sequence deletion. This indicates the predominant Sl in the host during the epidemic in the 1990s and after. The insertion cassettes suggested the mobilization from the Sls of other V. cholerae serogroups and Vibrio mimicus. Conclusion The study revealed that structural variations of Sis were obvious in the strains isolated in epidemics in different decades, whereas the divergence was based on syntenic structure of Sis in these El Tot strains. Also, the continuing cassette flows in the Sis of the host strains during the seventh cholera pandemics were displayed.

关键词： Superintegron Cassette Vibrio cholerae

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光锥耦合对ICCD成像系统分辨力的影响分析

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光学技术 2008年第6期34卷 803-805页

作者：崔志刚白廷柱高稚允北京理工大学信息科学技术学院光电工程系北京100081

对耦合过程中的每个环节的分辨力变化情况进行了实际测试和对比分析。阐述了制约ICCD分辫率的瓶颈因素,提出了通过耦合高清数码相机CCD来提高ICCD分辨力的方案。

关键词： ICCD 光锥耦合分辨力产生机理

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Cronobacter Carriage in Neonate and Adult Intestinal Tracts

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Biomedical and Environmental Sciences 2013年第10期26卷 861-864页

作者： LIU Hui cui Jing Hua cui zhi gang HU Guang Chun YANG Yue Lian LI Jian SHI Yu Wen Jinan Municipal Center for Disease Control and Prevention State Key Laboratory for Communicable Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention

A total of 7 Cronobocter strains were isolated from 703 fecal samples collected in Jinan from June 13 to December 30, 2011, with the positive rate of Cronobacter spp. being 1.0% （95% confidence interval 0.6%-1.4%）. Three Cronobacter sakazakii stains were isolated from 157 fecal samples of healthy neonates （95% confidence interval 0.4%-5.5%）. This number was slightly higher than that isolated from 273 fecal samples of healthy adults, in which 1 strain of C. sakazakii and 1 strain of Cronobacter malonaticus were isolated, and that from 173 fecal samples of adults with acute diarrhea, in which 1 strain of C sakazakii and 1 strain of C. malonaticus were isolated, but the differences were not statistically significant （P〉O.05）. The Cronobocter isolates were all from different genetic sources. It should be noted that Cronobacter carriage may cause infection under certain conditions, especially in neonates.

关键词： Cronobacter Carriage in Neonate and Adult Intestinal Tracts

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光锥耦合对ICCD响应不均匀性的影响分析

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北京理工大学学报 2007年第4期27卷 354-357页

作者：崔志刚白廷柱高稚允金伟其北京理工大学信息科学技术学院光电工程系北京100081

为提高光锥耦合ICCD成像系统的光响应均匀性,对光锥耦合过程造成光响应不均匀性的机理进行了分析,并用微观图解的方法清晰剖析不均匀性的产生机理.从CCD光敏元、光锥纤维、像增强器光纤面板输出面的微观结构出发,通过实测图像合成,模拟... 详细信息

为提高光锥耦合ICCD成像系统的光响应均匀性,对光锥耦合过程造成光响应不均匀性的机理进行了分析,并用微观图解的方法清晰剖析不均匀性的产生机理.从CCD光敏元、光锥纤维、像增强器光纤面板输出面的微观结构出发,通过实测图像合成,模拟再现了不均匀性的产生过程,分析了其主要因素,阐明了不均匀度的严重性,提出了通过减小光锥光纤直径改善ICCD的响应不均匀性的解决方案.

关键词：增强CCD 光锥耦合不均匀性产生机理

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基于HART协议的微差压智能变送器

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仪表技术与传感器 2005年第11期 22-23页

作者：崔志岗李小京天津工业大学计算机与自动化学院天津300160

在普通微差压变送器的基础上,设计了带有HART通讯协议的智能微差压变送器。介绍了整个智能变送器系统所涉及的硬件,阐述了系统的整体硬件结构以及通讯程序流程和数据采集流程。给出了部分的HART协议智能变送器的电路图,它可以实现HART... 详细信息

在普通微差压变送器的基础上,设计了带有HART通讯协议的智能微差压变送器。介绍了整个智能变送器系统所涉及的硬件,阐述了系统的整体硬件结构以及通讯程序流程和数据采集流程。给出了部分的HART协议智能变送器的电路图,它可以实现HART协议变送器的基本功能。

关键词： HART协议单片机智能变送器差压

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