Enzyme-Linked Immunosorbent Assay for Detecting Syphilis Dry Blood Spot Samples

作     者：CUI Zhi-gang DU Yan-li JIANG Yu-shan ZHENG Lu CUI Zhi-gang;DU Yan-li;JIANG Yu-shan;ZHENG Lu

作者机构：Department of Clinical LaboratoryHarbin Blood CenterHarbin Heilongjiang 150056China 

出 版 物：《Chinese Journal of Biomedical Engineering(English Edition)》 (中国生物医学工程学报（英文版）)

年 卷 期：2022年第31卷第2期

页      面：64-71页

学科分类：1002[医学-临床医学] 100206[医学-皮肤病与性病学] 10[医学] 

主　　题：syphilis blood spot sample enzyme-linked immunosorbent assay 

摘      要：Objective:To explore the feasibility of enzyme-linked immunosorbent assay(ELISA)in detecting syphilis dry blood ***:Based on dry blood spot samples,laboratory linear dial,laboratory basic dial,laboratory interference dial,and laboratory precision dial were *** linear range,sensitivity,specificity,precision and other performances of ELISA for detecting syphilis dry blood spot samples were comprehensively evaluated,and the stability of dry blood spot samples at 37℃was *** addition,112 suspected syphilis antibody-positive plasma samples were selected as the control,and dry blood spot samples were prepared *** clinical sensitivity,clinical specificity,positive predictive value and negative predictive value of dry blood spot samples and plasma samples in ELISA for syphilis detection were compared,and the consistency and correlation between the two samples were analyzed by the Kappa consistency test and Spearman rank correlation ***:The results of the linear analysis showed that the serial dilution of dry blood spot samples in ELISA for syphilis antibody ranged from 23to 27,and there was a good linear range[R2=0.9862(P0.05)].Sensitivity and specificity analysis showed that the positive coincidence rate and negative coincidence rate of the two detection methods were 100%(15/15).The results of the interference dial test showed that ELISA based on dry blood spot samples could accurately detect 6 syphilis antibody samples from 18samples,and the detection accuracy rate was 100.00%(6/6).The results of the precision test showed that the RSD of syphilis antibody detection in dry blood spot samples with different dilution times(23,25and 27)was 0.24%to 3.87%between spots,0.06%to4.07%between batches and 0.49%to 3.88%between *** 7 days,the inter-day RSD of dry blood spots with different dilution times(23,25,27)were 0.27%,0.65%and 0.95%,*** clinical sensitivity,clinical specificity,positive predictive value a