Structural Variation of the Superintegron in the Toxigenic Vibrio cholerae O1 El Tor

作     者：GAO Yan PANG Bo WANG Hai Yin ZHOU Hai Jian CUI Zhi Gang KAN Biao 

作者机构：State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable DiseaseControl and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2011年第24卷第6期

页      面：579-592页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 0903[农学-农业资源与环境] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by the National Natural Science Foundation of China (30800987) National Basic Research Priorities Program (2009CB522604) Priority Project on Infectious Disease Control and Prevention (2008ZX10004-009) 

主　　题：Superintegron Cassette Vibrio cholerae 

摘      要：Objective To understand the genetic structures and variations of the superintegron （Sl） in Vibrio cholerae isolated in the seventh cholera pandemic. Methods Polymerase chain reaction scanning and fragment sequencing were used. Sixty toxigenic V. cholerae O1 El Tor strains isolated between 1961 and 2008 were analyzed. Results Some variations were found, including insertions, replacements, and deletions. Most of the deletions were probably the result of recombination between V. cholerae repeat sequences. The majority of the variations clustered together. The Sis of the strains isolated in the 1960s and 1970s showed more diversity, whereas SI cassette variations in strains isolated in the 1990s and after were lower, with -24 kb signature sequence deletion. This indicates the predominant Sl in the host during the epidemic in the 1990s and after. The insertion cassettes suggested the mobilization from the Sls of other V. cholerae serogroups and Vibrio mimicus. Conclusion The study revealed that structural variations of Sis were obvious in the strains isolated in epidemics in different decades, whereas the divergence was based on syntenic structure of Sis in these El Tot strains. Also, the continuing cassette flows in the Sis of the host strains during the seventh cholera pandemics were displayed.