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A polyphenol-assisted IL-10 mRNA delivery system for ulcerative colitis

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Acta Pharmaceutica Sinica B 2022年第8期12卷 3367-3382页

作者： Zhejie chen Wei Hao Caifang gao Yangyang Zhou chen Zhang Jinming Zhang Ruibing Wang Yitao Wang Shengpeng Wang State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 999078China Macao Centre for Research and Development in Chinese Medicine University of MacaoMacao 999078China College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China

With the development of synthesis technology, modified messenger RNA(mRNA) has emerged as a novel category of therapeutic agents for a broad of diseases. However, effective intracellular delivery of mRNA remains challenging, especially for its sensitivity to enzymatic degradation. Here, we propose a polyphenol-assisted handy delivery strategy for efficient in vivo delivery of IL-10 ***-10 mRNA binds to polyphenol ellagic acid through supramolecular binding to yield a negatively charged core, followed by complexing with linear polyetherimide and coating with bilirubin-modified hyaluronic acid to obtain a layer-by-layer nanostructure. The nanostructure specifically up-regulated the level of IL-10, effectively inhibited the expression of inflammatory factors, promoted mucosal repair, protected colonic epithelial cells against apoptosis, and exerted potent therapeutic efficacy in dextran sulfate sodium salt-induced acute and chronic murine models of colitis. The designed delivery system without systemic toxicity has the potential to facilitate the development of a promising platform for mRNA delivery in ulcerative colitis treatment.

关键词： Polyphenol mRNA IL-10 Colitis Inflammatory bowel disease Drug delivery mRNA delivery Supramolecular binding

Meningeal lymphatic vessel crosstalk with central nervous system immune cells in aging and neurodegenerative diseases

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Neural Regeneration Research 2025年第3期20卷 763-778页

作者： Minghuang gao Xinyue Wang Shijie Su Weicheng Feng Yaona Lai Kongli Huang Dandan Cao Qi Wang Science and Technology Innovation Center Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Institute of Clinical Pharmacology Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina The Sixth Affiliated Hospital Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain metabolites but also contribute to antigen delivery and immune cell activation. The advent of novel genomic technologies has enabled rapid progress in the characterization of myeloid and lymphoid cells and their interactions with meningeal lymphatic vessels within the central nervous system. In this review, we provide an overview of the multifaceted roles of meningeal lymphatic vessels within the context of the central nervous system immune network, highlighting recent discoveries on the immunological niche provided by meningeal lymphatic vessels. Furthermore, we delve into the mechanisms of crosstalk between meningeal lymphatic vessels and immune cells in the central nervous system under both homeostatic conditions and neurodegenerative diseases, discussing how these interactions shape the pathological outcomes. Regulation of meningeal lymphatic vessel function and structure can influence lymphatic drainage, cerebrospinal fluid-borne immune modulators, and immune cell populations in aging and neurodegenerative disorders, thereby playing a key role in shaping meningeal and brain parenchyma immunity.

关键词： central nervous system meningeal lymphatic vessels immunity myeloid cells lymphatic cells neurodegenerative disease

Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro

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Journal of Integrative Agriculture 2021年第11期20卷 2914-2931页

作者： chen Li-li WANG Hao-ying GONG Xiao-chen ZENG Zhao-hai XUE Xu-zhang HU Yue-gao College of Science&Technology Ningbo UniversityCixi 315300P.R.China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193P.R.China Beijing Research Center for Intelligent Equipment in Agriculture Beijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China

Red and blue light illumination has been reported to significantly affect plantlet *** is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ***,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome *** objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq *** total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,*** plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under *** dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato *** addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato *** results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro

关键词： potato(Solanum tuberosum L.)plantlets in vitro red/blue LEDs light sources RNA-seq chlorophyll fluorescence plant hormone

从问题到实践:体育与健康跨学科主题学习的教学策略研究

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天津体育学院学报 2024年第3期39卷 302-308,317页

作者：张文鹏吴安月陈一林谭达顺高乙云南师范大学体育学院云南昆明650500 华东交通大学体育与健康学院江西南昌330013 兴义民族师范学院体育学院贵州兴义562400

加快推进体育与健康跨学科主题学习的关键在教学。运用文献资料、逻辑分析等方法,针对当前体育与健康跨学科主题学习中存在的问题,分析体育与健康跨学科主题学习的教学价值、教学困境与教学策略。研究认为,体育与健康跨学科主题学习的... 详细信息

加快推进体育与健康跨学科主题学习的关键在教学。运用文献资料、逻辑分析等方法,针对当前体育与健康跨学科主题学习中存在的问题,分析体育与健康跨学科主题学习的教学价值、教学困境与教学策略。研究认为,体育与健康跨学科主题学习的教学价值为培育时代新人的必然要求、落实“学、练、赛”一体化的有力抓手和培养学生高阶思维的动力源泉。当前,体育与健康跨学科主题学习的主要教学困境是教师知识储备不足、教学内容片面与情境失真、教学方法与手段单一、跨学科教学资源不足和教学评价操作性不强。从教学上提出优化策略:强化体育教师问题意识,提升跨学科教学能力;重视跨学科教学教材编写与情境创设,提升教学质量;以问题为导向探索跨学科教学模式,推动跨学科教学方法创新;增强协同效应获取各方支持,推动教学资源可持续发展;优化评价操作性,推动“教学评”一体化落地。

关键词：体育与健康课程跨学科主题学习跨学科主题教学教学实践教学策略

Single Ti atoms coupled with Ti-O clusters enable low temperature hydrogen cycling by sodium alanate

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Rare Metals 2024年第6期43卷 2671-2681页

作者： Zhuang-He Ren Xin Zhang Wen-Xuan Zhang Zhen-Guo Huang Li-Mei Yang Ya-Xiong Yang Zheng-Long Li Juan Li Wen-Ping Sun Ming-Xia gao Hong-Ge Pan Yong-Feng Liu State Key Laboratory of Silicon and Advanced Semiconductor Materials Zhejiang UniversityHangzhou 310027China School of Materials Science and Engineering Zhejiang UniversityHangzhou 310058China School of Civil&Environmental Engineering.University of Technology Sydney Sydney2007 NSWAustralia Institute of Science and Technology for New Energy Xi'an Technological UniversityXi'an 710021China College of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014China

Ti-based catalysts are known to improve the hydrogen storage performance of NaAlH4by facilitating the dissociation/recombination of H-H and Al-H *** catalytic activity of metallic Ti species strongly depends on its particle size and *** clusters and even single atoms are therefore highly desirable,but their controllable fabrication has been highly *** rein,we demonstrate a novel facile sonochemical synthesis of a Ti-O clusters featuring single Ti atom catalyst at room *** reducing TiCl_(4)by MgBu_(2)with ultrasound instead of heating as driving force,numerous single Ti atoms coupled with Ti-O clusters with Ti loading on graphene(Ti_(1)/Ti-O@G)up to 22.6 wt%have been successfully *** prepared Ti_(1)/Ti-O@G contributes high reactivity and superior catalytic activity,therefore enabling full dehydrogenation of NaAlH_(4)at 80℃in thermogravimetric mode and re-hydrogenation at 30℃and 10 MPa with 4.9 wt% H_(2).This fact indicates for the first time that single Ti atom catalyst with high loading is highly effective in catalyzing hydrogen cycling of NaAlH4at remarkably reduced temperatures.

关键词： Hydrogen storage Nanocatalysis Transition metals Single Ti atoms NaAlH_(4)

DNA methylation-dependent epigenetic regulationof Verticillium dahliae virulence in p

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aBIOTECH 2023年第3期4卷 185-201页

作者： Yun-Ya chen chen Zhu Jian-Hua Zhao Ting Liu Feng gao Ying-Chao Zhang cheng-Guo Duan Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences Chinese Academy of ScienceShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China College of Life Sciences Anhui Normal UniversityWuhu 241000China State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Qilu Zhongke Academy of Modern Microbiology Technology Jinan 250000China

As a conserved epigenetic mark, DNA cytosine methylation, at the 5’ position (5-mC), plays importantroles in multiple biological processes, including plant immunity. However, the involvement of DNAmethylation in the determinants of virulence of phytopathogenic fungi remains elusive. In this study,we profiled the DNA methylation patterns of the phytopathogenic fungus Verticillium dahliae, one of themajor causal pathogens of Verticillium wilt disease that causes great losses in many crops, and exploredits contribution in fungal pathogenicity. We reveal that DNA methylation modification is present in *** and is required for its full virulence in host plants. The major enzymes responsible for theestablishment of DNA methylation in V. dahliae were identified. We provided evidence that DNAmethyltransferase-mediated establishment of DNA methylation pattern positively regulates fungalvirulence, mainly through repressing a conserved protein kinase VdRim15-mediated Ca2? signaling andROS production, which is essential for the penetration activity of V. dahliae. In addition, we furtherdemonstrated that histone H3 lysine 9 trimethylation (H3K9me3), another heterochromatin markerthat is closely associated with 5-mC in eukaryotes, also participates in the regulation of V. dahliaepathogenicity, through a similar mechanism. More importantly, DNA methyltransferase genes VdRid,VdDnmt5, as well as H3K9me3 methyltransferase genes, were greatly induced during the early infection phase, implying that a dynamic regulation of 5-mC and H3K9me3 homeostasis is required for anefficient infection. Collectively, our findings uncover an epigenetic mechanism in the regulation ofphytopathogenic fungal virulence.

关键词： DNA methylation Verticillium dahliae Pathogenicity H3K9me3

基于实车试验的大跨度桥梁轨道静态长波高低不平顺验收标准验证

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铁道学报 2024年第1期46卷 120-128页

作者：李国龙孙宪夫高彦嵩杨飞高芒芒柯在田中国铁道科学研究院集团有限公司基础设施检测研究所北京100081 中国国家铁路集团有限公司科技和信息化部北京100844

由于温度、施工偏差、二期恒载等因素影响,高速铁路大跨度桥梁成桥线形与设计线形存在较大差异,且矢距差法不适用于大跨度桥梁和地基大面积沉降等区域的轨道线形验收。针对速度为250 km/h的高速铁路有砟轨道大跨度桥梁,基于轨道高低不... 详细信息

由于温度、施工偏差、二期恒载等因素影响,高速铁路大跨度桥梁成桥线形与设计线形存在较大差异,且矢距差法不适用于大跨度桥梁和地基大面积沉降等区域的轨道线形验收。针对速度为250 km/h的高速铁路有砟轨道大跨度桥梁,基于轨道高低不平顺不同弦测值与车体垂向振动加速度之间的相关性,提出高低不平顺60 m弦测值与车体垂向振动加速度之间的关联关系,进而给出桥上轨道静态高低长波不平顺60 m中点弦测值10 mm的验收限值建议,最后在某主跨为672 m的高速铁路斜拉桥上预设“四峰三谷”形式的轨道高低不平顺并进行实车验证,证明此限值建议的合理性。结果表明:对于速度为250 km/h的高速铁路,车体垂向振动加速度与不平顺60 m弦测值之间呈线性关系;大跨度桥梁温度变形显著,整体表现为桥面高程与温度成反相关关系,但桥梁温度变形主要表现为长波不平顺;预设的60 m余弦波形式的轨道高低不平顺主要影响行车舒适性,对安全性影响较小,且列车速度在275 km/h及以下时,产生的车体垂向振动加速度最大值为0.98 m/s2,舒适性指标最大为2.47,评价为“优秀”,接近优秀/良好分界线。

关键词：高速铁路大跨度桥梁预设不平顺实车试验轨道静态长波高低不平顺 60 m中点弦测法车体垂向加速度

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双级PIN限幅器的微波脉冲响应机理及规律

物理学报 2024年第6期73卷 349-359页

作者：高铭萱张洋张军国防科技大学前沿交叉学科学院长沙410073 国防科技大学理学院长沙410073

基于双级限幅器中两个PIN二极管的多物理场仿真模型与限幅器中其他电路元器件的SPICE模型,搭建了Si基双级PIN限幅器的场路协同仿真模型,利用这一模型对微波脉冲作用下限幅器中两级PIN二极管的温度响应特性进行了仿真.在此基础上对限幅... 详细信息

基于双级限幅器中两个PIN二极管的多物理场仿真模型与限幅器中其他电路元器件的SPICE模型,搭建了Si基双级PIN限幅器的场路协同仿真模型,利用这一模型对微波脉冲作用下限幅器中两级PIN二极管的温度响应特性进行了仿真.在此基础上对限幅器在不同频率、幅值微波脉冲信号作用下内部发生熔化现象所需的时间与能量进行了仿真,并对这一过程进行了机理分析与响应特性规律总结.仿真结果表明,当限幅器中第一级PIN二极管内部最高温度已达到材料熔点时,第二级PIN二极管的温度变化幅度较小.限幅器内部发生熔化现象所消耗的时间与能量随信号幅值、频率的变化呈现出规律性关系,发生熔化现象所需的时间随信号幅值或频率的提升而减小;发生熔化现象所需的能量随频率的提升而降低,随幅值的变化存在极大值点;限幅器的响应特性对信号参数表现出了不同的敏感性.

关键词：双级PIN限幅器微波脉冲场路协同仿真热效应

The in ovo injection of methionine improves intestinal cell proliferation and differentiation in chick embryos by activating the JAK2/STAT3 signaling pathway

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Animal Nutrition 2021年第4期7卷 1031-1038页

作者： Meng-Jie chen Jia-Yi Zhou Yi-Jun chen Xiu-Qi Wang Hui-Chao Yan Chun-Qi gao College of Animal Science South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Nutrition ControlGuangzhou510642China

The intestinal health of chick embryos is vital for their life-long growth,and exogenous nutrition intervention may provide sufficient nutrition for embryonic *** the present study,we investigated the effect of in ovo injection of L-methionine(L-Met)on the intestinal structure and barrier function of chick *** were 4 groups of treatments:the control(CON)group injected with phosphate-buffered saline(PBS)and the other 3 groups injected with 5,10,and 20 mg L-Met/egg,*** injection was performed on embryonic day 9(E9),and intestinal samples were collected on the day of hatching for *** results showed that,compared with the CON group,the groups administered an in ovo injection of L-Met increased relative weights of the duodenum,jejunum,and ileum(P<0.05).Hematoxylin and eosin(H&E)staining showed that the groups injected with 5,10,and 20 mg L-Met significantly increased villus height and crypt depth(P<0.05).Moreover,in ovo injection of 10 mg L-Met also increased the transepithelial electrical resistance(TEER)of the jejunum(P<0.05).Injection with 10 and 20 mg L-Met increased the expression of the tight junction proteins(ZO-1 and claudin-1)and the fluorescence signal intensity of Ki67 and villin proteins(P<0.05).Further,the protein expression of phospho-Janus kinase 2(p-JAK2)and phospho-signal transducer and activator of transcription 3(p-STAT3)was significantly increased by 10 or 20 mg L-Met injection(P<0.05).In conclusion,the injection of L-Met,especially at a dose of 10 mg,showed beneficial effects on the intestinal integrity of chick embryos due to the activation of the JAK2/STAT3 signaling *** results may provide new insights for regulating the intestinal development of embryonic chicks and the rapid growth of chicks after hatching.

关键词： In ovo injection Chick Intestine L-methionine JAK2/STAT3