Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro

作     者：CHEN Li-li WANG Hao-ying GONG Xiao-chen ZENG Zhao-hai XUE Xu-zhang HU Yue-gao CHEN Li-li;WANG Hao-ying;GONG Xiao-chen;ZENG Zhao-hai;XUE Xu-zhang;HU Yue-gao

作者机构：College of Science&TechnologyNingbo UniversityCixi 315300P.R.China College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China Beijing Research Center for Intelligent Equipment in AgricultureBeijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第11期

页      面：2914-2931页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：funded by the Scientific Research Fund of College of Science&Technology Ningbo University for the Introduction of High-level Talents China(RC190006) 

主　　题：potato(Solanum tuberosum L.)plantlets in vitro red/blue LEDs light sources RNA-seq chlorophyll fluorescence plant hormone 

摘      要：Red and blue light illumination has been reported to significantly affect plantlet *** is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ***,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome *** objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq *** total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched inphotosynthesisandphotosynthesis-antenna proteinsmetabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,*** plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under *** dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato *** addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in theplant hormone signal transductionmetabolic pathway,which were closely related to the endogenous plant hormone levels in potato *** results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro