DNA methylation-dependent epigenetic regulationof Verticillium dahliae virulence in p
作者机构：Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant SciencesChinese Academy of ScienceShanghai 200032China University of Chinese Academy of SciencesBeijing 100049China College of Life SciencesAnhui Normal UniversityWuhu 241000China State Key Laboratory of Plant GenomicsInstitute of MicrobiologyChinese Academy of SciencesBeijing 100101China Qilu Zhongke Academy of Modern Microbiology TechnologyJinan 250000China
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2023年第4卷第3期
页 面：185-201页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 0836[工学-生物工程]
基 金：funded by the National Natural Science Foundation of China(31770155) the Chinese Academy of Sciences(Grant No.XDB27040203)Cheng-Guo Duan
主 题：DNA methylation Verticillium dahliae Pathogenicity H3K9me3
摘 要：As a conserved epigenetic mark, DNA cytosine methylation, at the 5’ position (5-mC), plays importantroles in multiple biological processes, including plant immunity. However, the involvement of DNAmethylation in the determinants of virulence of phytopathogenic fungi remains elusive. In this study,we profiled the DNA methylation patterns of the phytopathogenic fungus Verticillium dahliae, one of themajor causal pathogens of Verticillium wilt disease that causes great losses in many crops, and exploredits contribution in fungal pathogenicity. We reveal that DNA methylation modification is present in *** and is required for its full virulence in host plants. The major enzymes responsible for theestablishment of DNA methylation in V. dahliae were identified. We provided evidence that DNAmethyltransferase-mediated establishment of DNA methylation pattern positively regulates fungalvirulence, mainly through repressing a conserved protein kinase VdRim15-mediated Ca2? signaling andROS production, which is essential for the penetration activity of V. dahliae. In addition, we furtherdemonstrated that histone H3 lysine 9 trimethylation (H3K9me3), another heterochromatin markerthat is closely associated with 5-mC in eukaryotes, also participates in the regulation of V. dahliaepathogenicity, through a similar mechanism. More importantly, DNA methyltransferase genes VdRid,VdDnmt5, as well as H3K9me3 methyltransferase genes, were greatly induced during the early infection phase, implying that a dynamic regulation of 5-mC and H3K9me3 homeostasis is required for anefficient infection. Collectively, our findings uncover an epigenetic mechanism in the regulation ofphytopathogenic fungal virulence.
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