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Agronomic evaluation of Herbaspirillum seropedicae strain ZAE94 as an inoculant to improve maize yield in Brazil

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Pedosphere 2021年第4期31卷 583-595页

作者： Gabriela c.ALVES carlos L.R.DOS SANTOS Jerri E.ZILLI Fabio B.DOS reis JUNIOR Ivanildo E.mARRIEL Farley A.da F.BREDA Robert m.BODDEY Veronica m.reis Federal Rural University of Rio de Janeiro Department of Soilskm 7 BR 465Seropédica RJ 23900-000(Brazil) Federal University of mato Grosso 2367 Fernando Corrêa Costa AvenueBoa Esperança M 78060-900(Brazil) Embrapa Agrobiology km 07 BR 465Seropédica RJ 23891-000(Brazil) Embrapa cerrados BR-020 km 18Planaltina DF 73310-970(Brazil) Embrapa maize and Sorghum MG 424 km 45Sete Lagoas MG 35701-970(Brazil)

Diazotrophic bacteria applied as a seed inoculant can improve the grain yield of several crops including maize. The current study aimed to test the agronomic efficiency and contribution of biological nitrogen fixation(BNF) of the endophytic diazotroph Herbaspirillum seropedicae strain ZAE94 to maize under field conditions. Eighteen field assays were conducted in four different locations during consecutive years on two hybrids and two varieties of maize in a random block design with four replicates using a peat-based inoculant. The inoculant containing the ZAE94 strain was applied without nitrogen(N)fertilization or with 40 kg N ha^(-1) and was compared to the application of 40 and 80 kg N ha^(-1) without inoculation. crop productivity and N accumulation in the grain were evaluated in addition to ^(15)N natural abundance(δ^(15)N) to evaluate BNF in the treatments without N fertilization. Fertilization at 40 kg N ha^(-1) plus bacterial inoculation produced crop yields similar to the treatment with 80 kg N ha^(-1) and increased grain N content, especially in the off-season with 40 kg N ha^(-1). The inoculation treatments showed lower δ^(15)N values than the non-inoculated treatments, which was most evident in the off-season. The BNF contributed about 30% of N accumulated in plants inoculated with ZAE94. On average, 64% of the N fertilized plots showed an increase of the parameters evaluated in the inoculated treatments, compared with the control. Inoculation also increased root length, root volume, and leaf area, and these parameters were positively correlated with plant weight using a hydroponic assay. This study revealed that the application of H. seropedicae inoculant increased the amount of N in plants owing to BNF, and there is a better chance of yield response to inoculation under low N fertilizer application in the off-season.

关键词： agronomic efficiency biological nitrogen fixation endophytic diazotroph inoculation 15N natural abundance plant growth-promoting bacteria

Shielding AZ91D-1%ca from corrosion through ultrasound melt treatment:A study for stent design

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Journal of magnesium and Alloys 2023年第8期11卷 2901-2915页

作者： I.V.Gomes m.Pacheco m.Nienaber S.c.Neves D.mei A.Barros R.L.reis J.L.Alves H.Puga cmEmS–Uminho University of MinhoGuimarãesPortugal LABBELS–Associate Laboratory BragaGuimarãesPortugal B's Research Group-Research Institute on Biomaterials Biodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAvepark-Parque de Ciência e TecnologiaBarco GuimarãesPortugal IcVS/3B's-PT Government Associate Laboratory BragaGuimarãesPortugal Institute of material and Process Design Helmholtz-Zentrum HereonGeesthacht 21502Germany School of materials Science and Engineering&Henan Key Laboratory of Advanced magnesium Alloy Zhengzhou UniversityZhengzhou 450001China

magnesium-based materials show great potential for producing biodegradable stents,but their high corrosion rates are a *** study investigates whether ultrasound melt treatment can change the corrosion response of an extruded AZ91D-1.0%ca(wt.%)in Earle's Balanced Salt Solution by tailoring the intermetallics'morphology in the as-extruded *** results showed that the wires from ultrasound-treated ingots corroded faster than non-treated ones in immersion for up to 6 *** trend shifted for longer periods,and ultrasound-treated material showed lower corrosion rates and uniform corrosion,while the non-treated material displayed localized corrosion *** testing of the wires demonstrated that immersion in EBSS lowered the tensile strength and elongation at fracture due to material degradation,regardless of the processing ***,this decline was sharper in the non-treated *** findings suggest that ultrasound melt processing can be a promising method for improving the corrosion resistance of magnesium-based materials,paving the way for their use in manufacturing biodegradable stents.

关键词： magnesium Ultrasound treatment Stent corrosion mechanical properties

Enabling affordances of blockchain in agri-food supply chains:A value-driver framework using Q-methodology

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International Journal of Innovation Studies 2023年第4期7卷 325-343页

作者： Pouyan Jahanbin Stephen c.Wingreen Ravishankar Sharma Behrang Ijadi marlon m.reis college of Business University of CanterburyChristchurch8140New Zealand college of Technological Innovation Zayed UniversityAbu DhabiUnited Arab Emirates AgResearch Ltd Lincoln7674New Zealand

The application of blockchain beyond cryptocurrencies has received increasing attention from industry and scholars *** predicted looming food crises,some of the most impactful deployments of blockchains are likely to concern food supply *** study outlined how blockchain adoption can result in positive affordances in the food supply *** Q-methodology,this study explored the current status of the agri-food supply chain and how blockchain technology could be useful in addressing existing *** theorization leads to the proposition of the 3TIc value-driver framework for determining the enabling affordances of blockchain that would increase shared value for ***,we propose a framework based on the most promising features of blockchain technology to overcome current challenges in the agri-food *** value-driver framework is driven by the Q-study findings of respondents closely associated with the agri-food supply *** framework can provide supply chain stakeholders with a clear perception of blockchain affordances and serve as a guideline for utilizing appropriate features of technology that match organizations’capabilities,core competencies,goals,and ***,it could assist top-level decision-makers in systematically evaluating parts of the organization to focus on and improve the infrastructure for successful blockchain implementation along the agri-food supply *** conclude by noting certain significant challenges that must be carefully addressed to successfully adopt blockchain technology.

关键词： Blockchain technology Agricultural food supply chain Q-methodology Trustworthy platform

An Outlook on Implantable Biosensors for Personalized medicine

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Engineering 2021年第12期7卷 1696-1699页

作者： Rita Rebelo Ana Isabel Barbosa Vitor m.correlo Rui L.reis Research Institute on Biomaterials Biodegradablesand BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineBarcoGuimarães 4805-017Portugal IcVS/3B’s-PT Government-Associated Laboratory BragaGuimarães 4710-057Portugal

Biosensors are a fast-growing field,as they have been shown to be very helpful in our daily life,playing roles in industries such as agriculture,food safety,homeland security,bioprocessing,environmental monitoring,and industrial *** these,the application of biosensing in medicine and biomedical engineering may have the highest potential for growth and for affecting human quality of life in the near *** potential is driven by the need for new and improved devices and technologies with improved sensitivity,specificity,reliability,and biocompatibility.

关键词： Outlook specificity playing

Replication of natural surface topographies to generate advanced cell culture substrates

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Bioactive materials 2023年第10期28卷 337-347页

作者： N.O.monteiro J.F.Fangueiro R.L.reis N.m.Neves 3B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkParque de Ciencia e Tecnologi1Zona Industrial da Gandr14805-017BarcoGuimaraesPortugal IcVS/3B’s-PT Government Associate Laboratory Brag1Guimar˜aesPortugal

Surface topographies of cell culture substrates can be used to generate in vitro cell culture environments similar to the in vivo cell niches. In vivo, the physical properties of the extracellular matrix (Ecm), such as its topography, provide physical cues that play an important role in modulating cell function. mimicking these properties remains a challenge to provide in vitro realistic environments for cells. Artificially generated substrates’ topographies were used extensively to explore this important surface cue. more recently, the replication of natural surface topographies has been enabling to exploration of characteristics such as hierarchy and size scales relevant for cells as advanced biomimetic substrates. These substrates offer more realistic and mimetic environments regarding the topographies found in vivo. This review will highlight the use of natural surface topographies as a template to generate substrates for in-vitro cell culture. This review starts with an analysis of the main cell functions that can be regulated by the substrate’s surface topography through cell-substrate interactions. Then, we will discuss research works wherein substrates for cell biology decorated with natural surface topographies were used and investigated regarding their influence on cellular performance. At the end of this review, we will highlight the advantages and challenges of the use of natural surface topographies as a template for the generation of advanced substrates for cell culture.

关键词： Biomimetic topography cell culture substrates Natural surface topographies

Neurochemical plasticity of müller cells after retinal injury: overexpression of GAT-3 may potentiate excitotoxicity

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Neural Regeneration Research 2015年第9期10卷 1376-1378页

作者： clarissa S.Schitine Fernando G.de mello Ricardo A.m.reis Laboratório de Neuroquímica Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Laboratório de Neuroanatomia celular Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro

The retina is a multilayered tissue that develops following a central-to-peripheral gradient. Its structure derives from multipotent precursors, as shown through clonal analysis of retinal cell lineage. These progenitors generate diverse cell types, controlled by complex influences of intrinsic and extrinsic factors （Hatakevama and Kagevama, 2004）.

关键词： GAT Neurochemical plasticity of m ller cells after retinal injury overexpression of GAT-3 may potentiate excitotoxicity RGcs

Towards an integrated framework for the risk assessment of coastal structures exposed to earthquake and tsunami hazards

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Resilient cities and Structures 2022年第2期1卷 57-75页

作者： cláudia reis mário Lopes maria Ana Baptista Stéphane clain civil Engineering Research and Innovation for Sustainability CERISInstituto Superior TécnicoUniversidade de LisboaAvenida Rovisco Pais1Lisboa 1049-001Portugal Instituto Superior de Engenharia de Lisboa Instituto Politécnico de LisboaR.Conselheiro Navarro 1Lisboa 1959-007Portugal Departamento de matemática Centro de FísicaUniversidade do MinhoCampus de GualtarBraga 4710-057Portugal

The spatial distribution of the world population is uneven,with a density of about 40%living in coastal *** trend is expected to continue in both demographic indicators and urban development rate,being many coastal cities in seismic-and tsunami-prone regions and built through informal and unplanned settlements,exposing their population and assets to such *** tectonic-triggered events raised awareness of the cascading earthquake and tsunami threat and highlighted the paucity of structural design criteria considering the cumulative effects of *** being exposed to the ground-motion,the structures’resistance may decrease and become residual/non-existent to support the incoming tsunami,implying an underestimation of the *** management can benefit from reinforcing the ties between natural hazards and engineering practitioners,linking science and industry,and promoting dialogue between risk analysts and *** by the expansion plans of an internationally-sized deep-water port located in a tsunami-prone region,a reflection on the work needed to perform a multi-risk assessment and the challenges yet to overcome is introduced to emphasize the challenge of combining safety requirements with financial and ecologic concerns.A conceptual interdisciplinarybased methodology is proposed to support uncertainty-aware,systematic and informed decisions.

关键词： cascading earthquake and tsunami multi-hazard Structural vulnerability to earthquake and derivative tsunami Performance-based engineering to cascading ground motion and tsunami

^(222)Rn calibration procedure for water analyses by liquid scintillation counting

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Radiation Detection Technology and methods 2023年第2期7卷 311-319页

作者： Ana Rita Gomes Sérgio Dias Anabela mourato Joao Abrantes mário reis Laboratório de Protecao e Seguranca Radiológica Instituto Superior TécnicoUniversidade de LisboaEstrada Nacional 10km 13972695‑066 Bobadela LRSPortugal Laboratórios de massa e massa Volúmica de Sólidos Instituto Portugues da QualidadeRua António Giao22829‑513 CaparicaPortugal centro de ciências e Tecnologias Nucleares Instituto Superior TécnicoUniversidade de LisboaEstrada Nacional 10km 13972695‑066 Bobadela LRSPortugal

Purpose Among the several methods to measure ^(222)Rn in water,the liquid scintillation counting(LSc)technique is one of the most sensitive and widely used when analysing non-saline waters by liquid extraction,providing excellent accuracy,precision and low-level detection *** using the liquid scintillation counter Tri-carb 3170 TR/SL(Perkin-Elmer)it is not possible to make an automatic determination of the alpha/beta discrimination parameter,being necessary to evaluate the pulse decay discriminator(PDD)value manually to the ^(222)Rn *** this work,we describe the steps to perform the calibration of that type of *** The method consists in the preparation of standard samples from a ^(226)Ra certified standard solution and the adjustment of the PDD value by hand until the percentage of alpha events reaches a plateau of a near-constant fraction of total *** process consists in taking several measures between PDD 80 and 200 with 15 min counting time *** the plateau,the alpha/beta ratio was used to determine the plateau final range as well as the optimum PDD *** that,the calibration standards and blanks were measured and the region of interest(ROI)was established by finding the maximum value for the figure of merit(FOm)in the upper and lower *** The optimal PDD value of 148 was established by the alpha/beta ratio,and after that,it was possible to measure ^(226)Ra standards to perform the *** efficiency of(9.758±0.035)×1^(0-1) was *** routine measurements,a detection limit of about 0.50 Bq L^(-1) is achieved for a 120 min counting *** evaluate the uncertainty budget,the uncertainties of the net count rate,the efficiency,the sample amount and the decay correction factor were *** A method was developed for the calibration of LSc Tri-carb 3170 TR/SL with the optimization of the PDD using a ^(226)Ra standard *** acquisition to obtain the plot of the variation

关键词： Radon Waters Pulse decay discriminator Liquid scintillation counting calibration

Interaction between cannabinoid and nucleotide systems as a new mechanism of signaling in retinal cell death

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Neural Regeneration Research 2019年第12期14卷 2093-2094页

作者： Hércules R. Freitas Ricardo A. m. reis Ana L. m. Ventura Guilherme R. Franca Laboratory of Neurochemistry Institute of Biophysics Carlos Chagas Filho Universidade Federal do Rio de Janeiro Rio de Janeiro RJ Brazil School of Health Sciences Centro Universitário IBMR Rio de Janeiro RJ Brazil Laboratory of Neurochemistry Institute of Biology Universidade Federal Fluminense Niterói RJ Brazil Biomedical Institute Departament of Physiological Sciences/ Pharmacology Universidade Federal do Estado do Rio de Janeiro Rio de Janeiro RJ Brazil

Nervous system emerges from complex signaling interactions where extrinsic (neurotransmitters and trophic factors, among others) and intrinsic factors (transcription factors) interplay in the developing tissue to control gene activity promoting chronic changes in cell genesis, migration, differentiation and death. The retinal microenvironment is regulated by a broad variety of chemicals, including endocannabinoids and nucleotides that modulate embryonic progenitor-neuron-müller glia signaling in very early developing or pathophysiological conditions.

关键词： Interaction nucleotide systems cell death