Replication of natural surface topographies to generate advanced cell culture substrates

作     者：N.O.Monteiro J.F.Fangueiro R.L.Reis N.M.Neves 

作者机构：3B’s Research GroupI3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkParque de Ciencia e Tecnologi1Zona Industrial da Gandr14805-017BarcoGuimaraesPortugal ICVS/3B’s-PT Government Associate LaboratoryBrag1Guimar˜aesPortugal 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第28卷第10期

页      面：337-347页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This work is a result of the project FROnTHERA(NORTE-01-0145-FEDER-000023) supported by Norte Portugal Regional Operational Programme(NORTE 2020) under the PORTUGAL 2020 Partnership Agreement,through the European Regional Development Fund(ERDF) Portuguese Foundation for Science and Technology under the doctoral programme in Tissue Engineering,Regenerative Medicine and Stem Cells(PD/59/2013) PD/BD/128087/2016 by the project Cells4_IDs(PTDC/BTM-SAL/28882/2017) 

主　　题：Biomimetic topography Cell culture substrates Natural surface topographies 

摘      要：Surface topographies of cell culture substrates can be used to generate in vitro cell culture environments similar to the in vivo cell niches. In vivo, the physical properties of the extracellular matrix (ECM), such as its topography, provide physical cues that play an important role in modulating cell function. Mimicking these properties remains a challenge to provide in vitro realistic environments for cells. Artificially generated substrates’ topographies were used extensively to explore this important surface cue. More recently, the replication of natural surface topographies has been enabling to exploration of characteristics such as hierarchy and size scales relevant for cells as advanced biomimetic substrates. These substrates offer more realistic and mimetic environments regarding the topographies found in vivo. This review will highlight the use of natural surface topographies as a template to generate substrates for in-vitro cell culture. This review starts with an analysis of the main cell functions that can be regulated by the substrate’s surface topography through cell-substrate interactions. Then, we will discuss research works wherein substrates for cell biology decorated with natural surface topographies were used and investigated regarding their influence on cellular performance. At the end of this review, we will highlight the advantages and challenges of the use of natural surface topographies as a template for the generation of advanced substrates for cell culture.