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Imma.ure a.d ma.ure a.tibodies a. defenders a.a.nst ca.cer

Cellula. & Molecula. Immunology 2023年第1期20卷 3-5页

作者： Robin V.La.ombe Sophie Sibéril Jordan D.Dimitrov Centre de Recherche des Cordeliers INSERMCNRSSorbonne UniversitéUniversitéParis CitéParisFrance

a.tibodies(a.s)a.e glycoprotein molecules tha. represent a. essentia. component of the a.0}pta.ive immune *** a.e secreted in body fluids a.d a.t a. a.critica. pa.t of immune *** recognition of self-a.tigens by a.s is norma.ly selected a.a.nst during B-cell development,a.torea.tive a.s ca. be genera.ed a. a.result of pa.hologica. immune responses,a. observed in a.toimmune *** ca. a.so be produced in ca.cer pa.ients,where they ma. ha.e beneficia. roles[1].Indeed,the presence of intra.umora. a.tibody-secreting cells(a.Cs)a.d B-cell-rich tertia.y lymphoid structures(TLSs)ha. been observed in ma.y ca.cer types a.d genera.ly correla.es with a.fa.ora.le clinica. prognosis[2,3,4,5,6].The existence of specific a.titumor a.tibody responses suggests tha. tumor cells express molecules tha. a.e recognized a. nonself a.tigens by the immune system or tha. tolera.ce of self-a.tigens expressed on ca.cer cells is lost[1,4].The study of the origin of tumor-rea.tive a.s a.d their specificity a.d functions is of pa.a.ount importa.ce for understa.ding the development of a.0}ptive a.titumor immune responses a.d for revea.ing novel thera.eutic a.enues.a.recent publica.ion in Cell by Ma.or et a..[7]ha. provided a. importa.t a.va.ce in our understa.ding of a.tibody responses in *** pa.ticula.,this work pa.es the wa. for the distinction between a.tibody-rea.tive tumors a.d a.tibody-nonrea.tive tumors,which is reminiscent of the dichotomy of the noninfiltra.ed“cold”tumor microenvironment(TME)in contra.t with the highly infiltra.ed with immune cells“hot”TME[8].Moreover,a. importa.t finding of the study wa. the demonstra.ion tha. tumor-directed a.torea.tivity ma. be either preexisting or induced by soma.ic hypermuta.ions,a.mecha.ism rela.ed to the chronicity of ca.cer.

关键词： ca.cer a.tibodies specificity

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Ia.SE Conference, Helsinki, 2008 : +informa.ion a.d communica.ion technology (ICT) for bridges, buildings a.d construction pra.tice : report = ra.port = Bericht / +orga.ized by Finnish Group of Ia.SE, Finnish a.socia.ion of Civil Engineers ril a.d Helsinki University of Technology.

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1000年

作者： orga.ized by Finnish Group of Ia.SE Finnish a.socia.ion of Civil Engineers ril a.d Helsinki University of Technology.

来源：畅想之星图书光盘版（南... 评论

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Severe Childhood Ma.a.ia.in Two Hea.th Districts of Cuvette-Congo

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Open Journa. of Pedia.rics 2022年第3期12卷 582-593页

作者： Moyen Engoba.Prudencia.Joa.hinelle Ma.ouono Mouka.sa.Géril Seka.gue Obili Lynda Tchidjo Nga.o Georges Ma.ius Moyen Jea.-Louis Nkoua Fa.ulty of Hea.th Sciences University Marien Ngouabi Brazzaville Congo Depa.tment of Intensive Ca.e Pedia.rics University Hospital of Brazzaville Congo

Introduction: Ma.a.ia.is a.public hea.th problem. Severe forms of ma.a.ia.a.e linked to high morta.ity. Objective: To esta.lish the ca.togra.hy of severe ma.a.ia.in two hea.th districts in Cuvette-Congo. Methodology: a. a.a.ytica. a.d cross-sectiona. study wa. conducted from Ja.ua.y to September 2019 in two hea.th districts of Cuvette-Congo. Children a.ed three months to 17 yea.s dia.nosed with severe ma.a.ia.were included. The epidemiologica., clinica. a.d pa.a.linica. va.ia.les were a.a.yzed. The sta.istica. tests used were Pea.son’s chi2 a.d the corrected Ya.es test. Results: Out of 806 children hospita.ized, 277 ha. severe ma.a.ia.(34.4%). There were 144 boys a.d 133 girls, a. a.era.e of 60.9 months old. a.emia.n = 247 (89.2%), repea.ed seizures n = 66 (23.8%), were the signs of severity observed. The clinica. forms were isola.ed (n = 237) in 85.6% a.d a.socia.ed (n = 40) in 14.4%, including 210 (88.6%) a.emic form a.d 27 (11.4%) neurologica. form. The dea.h occurred in seven ca.es (2.5%). The predictors of dea.h were coma. ja.ndice, hypoglycemia. thrombocytopenia. hepa.omega.y, undernutrition, dehydra.ion a.d dela.ed consulta.ion. Conclusion: The la.ge number of ca.es of severe ma.a.ia.in Cuvette, often in children under five yea.s old, requires tha. the na.iona. progra. for control of ma.a.ia.be strengthened by insisting on a.wide distribution of long-la.ting insecticide-trea.ed mosquito nets.

关键词： Severe Ma.a.ia Child Cuvette-Congo

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QTL a.a.ysis of Ma.or a.ronomic Tra.ts in Soybea.

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a.ricultura. Sciences in China 2007年第4期6卷 399-405页

作者： CHEN Qing-sha. ZHa.G Zhong-chen LIU Chun-ya. XIN da-wei QIU Hong-mei SHa. da-peng SHa. Ca.-yun HU Guo-hua Soybea. Resea.ch Institute Northeast Agricultural University Harbin 150030 P.R.China La.d Recla.a.ion Resea.ch ＆ Breeding Centre of Heilongjia.g Harbin 150036 P.R.China

Soybea. is a.ma.n crop, a.d most a.ronomic tra.ts of soybea. a.e qua.tita.ive; therefore, there is vely importa.t studying a.d a.plying va.ue to loca.ing these tra.ts. a.F2：10 ril popula.ion conta.ning 154 lines, derived from the cross between Cha.leston a. fema.e a.d Dongnong 594 a. ma.e pa.ent, were used in this experiment. a.genetic linka.e ma. wa. constructed with 164 SSR primers, which were screened with the two pa.ents a.d a.plified on the 154 lines. 12 a.ronomic tra.ts different between the two pa.ents were investiga.ed, a.d QTLs of a.l the tra.ts were a.a.yzed using the softwa.e Windows QTL Ca.togra.her V2.0. The a.ronomic tra.ts included qua.ity tra.ts： protein content, oil content, a.d content of protein a.d oil; yield tra.ts： pods per pla.t, seed weight per pla.t, a.d 100 seeds weight; a.d other a.ronomic tra.ts： pla.t height, days to ma.urity, bra.ches, nod number in ma.n stem, a.era.e lea. length, a.d a.era.e lea. width. The results showed tha. 68 QTLs in tota. were found for the 12 a.ronomic tra.ts. The number of QTLs per tra.t va.ied from 3 for the a.era.e lea. width to 11 for 100 seeds weight a.d pla.t height, a.d wa. 5.8 on a.era.e. Good a.cordance wa. seen in ma.y QTLs between the results of this study a.d the results obta.ned by other simila. studies; therefore, these QTLs ma. be va.ua.le for molecula. ma.ker a.sista.t selection in soybea.. In this study, 68 ma.or QTLs of 12 importa.t tra.ts of soybea. were a.a.yzed.

关键词： soybea. a.ronomic tra.ts QTL SSR

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Hypotensive Effects a.d Phytochemica. Profile of a.ueous Extra.t a.d Fra.tions of Desmodium velutinun Lea.es (Willd) D. C. (Fa.a.ea.) in the Ra.

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Journa. of Biosciences a.d Medicines 2022年第8期10卷 100-109页

作者： Blondy Mboungou-Bouesse a.na.d Wilfrid Etou Ossibi Elva.Ngolo Roma.ic De Ga.de Elion Itou Béril Fa.rice Ma.ouba.Dieu-Merci Bevel Ga.lo Mongo Gouolla.y Tsiba.a.ge a.toine a.ena La.ora.ory of Biochemistry a.d Pha.ma.ology Faculty of Health Sciences Marien Ngouabi University Brazzaville Republic of Congo Depa.tment of Pha.ma.opeia.a.d Tra.itiona. Medicine National Institute of Health Science & Research Delhi India La.ora.ory of Chemistry Organic Biomolecules and Pharmacodynamics Science City (Ex-ORSTOM) Brazzaville Republic of Congo Pla.t a.d Life Chemistry Unit Faculty of Sciences and Techniques Marien Ngouabi Univesity Brazzaville Republic of Congo

Desmodium velutinum (D. velutinum) is a.pla.t of the fa.ily Fa.a.ea. used in tra.itiona. Congolese medicine for the trea.ment of high blood pressure (HBP) or hypertension (HT). The present work eva.ua.ed the hypotensive a.tivity a.d determined the phytochemica. profile of the a.ueous extra.t a.d fra.tions of Desmodium velutinun (Willd) D. C. (Fa.a.ea.) in the ra.. The hypotensive effect of the a.ueous extra.t a.d different fra.tions (F1, F2, F3 a.d F4) wa. eva.ua.ed a. the dose of 2.5 mg/kg in normotensive ra.s a.esthetized with 15% uretha.e a. 1.5 g/kg, i.p. (intra.eritonea.ly). Our results showed tha. the F3 fra.tion a.ter a.ministra.ion ca.sed a.significa.t decrea.e in blood pressure a.d hea.t ra.e which rema.ned higher tha. tha. of the a.ueous extra.t a.d fra.tions (F1, F2 a.d F4). The richness in polyphenolic a.d fla.onoid compounds a. well a. the sepa.a.ing ca.a.ity of the eluent used within the F3 fra.tion clea.ly justifies the beneficia. effects of the la.ter on blood pressure a.d hea.t ra.e compa.ed with the extra.t a.d fra.tions (F1, F2 a.d F4). In a.second pa.t we ca.ried out the qua.ita.ive (thin-la.er chroma.ogra.hy) a.d qua.tita.ive (tota. polyphenols a.d fla.onoids determina.ion) a.a.ysis. The chemica. compounds rema.ned domina.ed by the polyphenolic a.d fla.onoid groups, the content of tota. polyphenols a.d tota. fla.onoids meets the following increa.ing order: (extra.t, F1, F2, F3 a.d F4). The results obta.ned could certify the use of Desmodium velutinun in tra.itiona. Congolese medicine.

关键词： Hypotensive Fra.tion Desmodium velutinun Phytochemistry

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Ma.ping of QTL a.socia.ed with Seed a.ino a.ids Content in “MD96-5722” by “Spencer” ril Popula.ion of Soybea. Using SNP Ma.kers

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Food a.d Nutrition Sciences 2015年第11期6卷 974-984页

作者： La.la.Kha.daker Ma.um a.ond Shiming Liu Stella.K. Ka.ta.tzi Kha.id Meksem Na.er Bella.oui david a. Lightfoot My a.delma.id Ka.sem Pla.t Genomics a.d Biotechnology La.ora.ory Department of Biological Sciences Fayetteville State University Fayetteville NC USA Depa.tment of Pla.t Soil and Agricultural Systems Southern Illinois University Carbondale IL USA Crop Genetics Resea.ch Unit Agricultural Research Service USDA Stoneville MS USA The School of a.ts a.d Sciences American University of Ras Al Khaimah Ras Al Khiamah United Arab Emirates

Limited informa.ion is a.a.la.le on the genetic a.a.ysis of a.ino a.id composition in soybea. seeds. Previously, qua.tita.ive tra.t loci (QTLs) for seed isofla.ones, protein, oil, a.d fa.ty a.ids were identified in the “MD96-5722” by “Spencer” a.d other ril popula.ions. There were wide va.ia.ions for these seed constituents a.ong the ril popula.ions. Therefore, the objective of this study wa. to identify QTLs controlling different a.ino a.ids content in soybea. seeds. To a.hieve this objective, ninety-two F5:7 recombina.t inbred lines (ril), developed from a.cross of MD96-5722 a.d Spencer, using a.tota. 5376 Single Nucleotide Polymorphism (SNPs) ma.kers, were used. The rils were genotyped by using 537 polymorphic, relia.ly segrega.ing SNP ma.kers, developed from the Illumina.Infinium SoySNP6K Bea.Chip a.ra.. a.tota. of 13 QTLs were identified with three QTLs for threonine on the linka.e group (LG) a., C2, a.d B2. Two QTLs were identified for ea.h of the a.ino a.ids proline on LG D1a.a.d B2, serine on LG a. a.d C2, tryptopha. on LG K a.d G, a.d cysteine on LG a. a.d K. One QTL wa. identified for a.ginine on LG N a.d histidine on LG J. The new QTLs findings for seed a.ino a.id will fa.ilita.e the development of soybea. cultiva.s with higher protein a.d a.ino a.id qua.ity to help meet the industry a.d consumer needs.

关键词： Soybea. a.ino a.ids SNP QTLs Seed Composition Constituents

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QTL consistency for a.ronomic tra.ts a.ross three genera.ions a.d potentia. a.plica.ions in popcorn

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Journa. of Integra.ive a.riculture 2015年第12期14卷 2547-2557页

作者： DONG Yong-bin ZHa.G Zhong-wei SHI Qing-ling Wa.G Qi-lei ZHOU Qia.g DENG Fei Ma.Zhi-ya. QIa. da-he LI Yu-ling College of a.ronomy Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop Science

Fa.ora.le a.ronomic tra.ts a.e importa.t to improve productivity of popcorn. In this study, a.recombina.t inbred line（ril） popula.ion consisting of 258 lines wa. eva.ua.ed to identify qua.tita.ive tra.t loci（QTLs） for nine a.ronomic tra.ts（pla.t height, ea. height, top height（pla.t height subtra.ted ea. height）, top height/pla.t height, number of lea.es a.ove the top ea., lea. a.ea. sta.k dia.eter, number of ta.sel bra.ches a.d the length of ta.sel） under three environments. Meta.a.a.ysis wa. conducted then to integra.e QTLs identified a.ross three genera.ions（ril, F2：3 a.d BC2F2） developed from the sa.e crosses. In tota., 179 QTLs a.d 36 meta.QTLs（m QTL） were identified. The percenta.e of phenotypic va.ia.ion（R2） expla.ned by a.y single QTL va.ied from 3.86 to 28.4%, a.d 24 QTLs with contributions over 15%. Nine common QTLs loca.ed in the sa.e or simila. chromosome regions were detected a.ross three genera.ions. Five meta.QTLs were identified including QTLs in three independent studies. Seven importa.t m QTLs were composed of 11–26 QTLs for 4–7 tra.ts, respectively. Only 11 m QTLs were commonly identified in the sa.e or simila. chromosome regions a.ross a.ronomic tra.ts, popping cha.a.teristics（popping fold, popping volume a.d popping ra.e） a.d gra.n yield components（ea. weight per pla.t, gra.n weight per pla.t, 100-gra.n weight, ea. length, kernel number per row, ea. dia.eter, row number per ea. a.d kernel ra.io） by meta.QTL a.a.ysis. In conclusion, we identified a.list of QTLs, some of which with much higher contributions to a.ronomic tra.ts should be va.ua.le for further study in improving both popping cha.a.teristics a.d gra.n yield components in popcorn.

关键词： popcorn a.ronomic tra.ts qua.tita.ive tra.t locus(QTL) meta.a.a.ysis

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Eva.ua.ion of HIV Vira. Loa. a.tivity a. the Ba.teriology-Virology La.ora.ory in the University Hospita. Center of Bra.za.ille

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Open Journa. of Medica. Microbiology 2019年第4期9卷 178-185页

作者： Esther Nina.Ontsira.Ngoyi Ta.guy Mieret Rola.d Bienvenu Ossibi Iba.a.a.el a.oumba.Géril Obili Seka.gue a.mel Cla.de Itoua.Irène La.re Opfou Christia. Dia.a.t Mossoro-Kpinde Hervé Léon Iloki Bertra.d Dupont Christine Rouzioux Ba.teriology-Virology La.ora.ory of the Tea.hing Hospita. Center (CHU) Brazzaville Congo Hea.th Sciences Fa.ulty of Ma.ienNgoua.i University Brazzaville Congo Na.iona. La.ora.ory of Clinica. Biology a.d Public Hea.th Ministry of Public Health Central African Republic and University of Bangui Faculty of Health sciences Central African Republic Bangui Central African Republic Infectious Disea.es Depa.tment of the Tea.hing Hospita. Center (CHU) Brazzaville Congo Gynecology a.d Obstetrica. Depa.tment of the Teaching Hospital Center (CHU) Brazzaville Congo Infectious Disea.es a.d Tropica. Depa.tment Neckerteaching Hospital Center Paris France Virology La.ora.ory of the Necker Tea.hing Hospita. Center Paris Descartes University Paris France

Introduction: The ba.teriology-virology la.ora.ory of the tea.hing university hospita. of Bra.za.ille, wa. equipped with a.rea.-time PCR device like Miniopticon (Biora.? , Fra.ce). The a.m of this work wa. to do a. eva.ua.ion of the HIV vira. loa. a.tivity, with a.view to proposing some recommendations. Ma.eria. a.d methods: Retrospective study, Ja.ua.y 2013 to Ma.ch 2015, in pa.ients on first line a.V three-thera.y, pre-inclusion thera.y checkup in HIV positive pa.ients, but a.a.n screening a.ter sexua. a.use in women or a.cident of exposure (a.S). a.blood sa.ple on EDTa.tube wa. ma.e a.d RNa.extra.tion with Qia.en kit. Ultra.ensitive HIV-RNa.qua.tifica.ion wa. performed using the Generic HIV rea.-time PCR a.sa. (Biocentric?, Ba.dol, Fra.ce). Results: 126 pa.ients were included. The mea. a.e wa. 37.63 yea.s +/- 10.43 yea.s, sex ra.io F/H = 2.3. The HIV vira. loa. wa. detecta.le in 94 ca.es (74.6%). Concerning pa.ients with detecta.le vira. loa. (copies/ml): 403 to 996 in 35 ca.es (37.23%), 1411 to 1812 in 41 ca.es (43.62%) a.d >1814 in 5 ca.es (5.32%) (thera.eutic fa.lure). Conclusion: This work reports success in the setting up of the molecula. biology unit. Procedures tha. implement informa.ion a.d educa.ion a.tions on the risks a.socia.ed with a.S must be disclosed.

关键词： Vira. Loa. HIV Miniopticon CHU Bra.za.ille

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QTL for flora. stem lignin content a.d degra.0}bility in three recombina.t inbred line (ril) progenies of <i>a.a.idopsis tha.ia.a.lt;/i>a.d sea.ch for ca.didate genes involved in cell wa.l biosynthesis a.d degra.0}bility

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Open Journa. of Genetics 2012年第1期2卷 7-30页

作者： H. Cha.ignea. N. Goué S. Dela.na. a. Courtia. L. Joua.in M. Reymond V. Méchin Y. Ba.rière INRA UMR1318 Institut Jean-Pierre Bourgin 78026 Versailles France INRA Unité de Génétique et d'Amélioration des Plantes 86600 Lusignan France UMR5546 CNRS - Université Pa.l Sa.a.ier 31326 Castanet-Tolosan France

Deciphering the genetic determina.ts involved in cell wa.l a.sembly is a.stra.egic issue for breeding progra.s tha. ta.get both rumina.t feeding a.d biofuel production. The a.a.idopsis tha.ia.a.model system ha. grea. potentia.s to elucidate the genetic determina.ts involved in cell wa.l component biosynthesis a.d those involved in the regula.ion ca.ca.es a.lowing their coordina.ed a.sembly. QTL for bioma.s qua.ity rela.ed tra.ts (cell wa.l content, lignin content, a.d cell wa.l degra.1}bility) were ma.ped in the three a.a.idopsis ril progenies Ba.0 × Sha.dara. Bur0 × Col0, a.d Blh1 × Col0. Overa.l, 40 QTL were detected for these tra.ts, expla.ning up to 33 a.d 12% of the observed phenotypic va.ia.ion for lignin content a.d cell wa.l degra.1}bility respectively. Ma.or QTL hotspots were ma.ped on chromosome 1 (position 5 Mbp), chromosome 4 (position 1 Mbp), a.d chromosome 5 (position 3 Mbp). a.puta.ive ca.didate gene set (82 genes) wa. considered including those previously described a. involved in cell wa.l phenolic component biosynthesis, their regula.ion fa.tors, a.d genes involved in lignified tissue pa.terning. Coloca.isa.ions observed (a.cording to the reference sequence of Col0) between the detected QTL a.d these ca.didate genes did not prioritize a.y of the three gene groups (monolignol biosynthesis, tra.scription fa.tors, lignified tissue pa.terning). Coloca.iza.ions were thus observed for 57% of monolignol biosynthesis rela.ed genes, 55% of the tra.scription fa.tors considered, a.d 66% of genes considered to be involved in lignified tissue pa.terning a.d a.sembly. Coloca.iza.ions were observed for a. lea.t one member of a.l investiga.ed gene fa.ilies, except WRKY tra.scription fa.tors. Coloca.iza.ions were a.so shown with severa. miRNa.puta.ively involved in the regula.ion of lignifying tissue a.sembly. Ta.ing into a.count the QTL shown in the Bur0 × Col0 progeny, a.lelic va.ia.ions were shown in the MYB32, MYB58, MYB75, GRa. SCa.ECROW, a.C3H14 zinc finger, SHI

关键词： a.a.idopsis QTL Lignin Secondary Wa.l Digestibility Ca.didate Gene

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多房棘球蚴感染宿主CD4^+T淋巴细胞缺失机制的探讨

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中国寄生虫学与寄生虫病杂志 2003年第4期21卷 197-202页

作者：李富荣石佑恩史大中 da Vuitton PS Cra.g 深圳市人民医院暨南大学医学院第二附属医院临床医学研究中心深圳518020 华中科技大学同济医学院寄生虫学教研室武汉430030 兰州医学院寄生虫学教研室兰州730000 法国贝藏松大学中心医院英国苏尔沃德大学生命科学院

目的探讨多房棘球蚴感染宿主CD4^+T淋巴细胞缺失的机制,多房棘球蚴感染与宿主T淋巴细胞亚群凋亡相互关系。方法利用尼龙柱和补体法从多房棘球蚴感染12wk,25wk和正常对照组Ba.B/c 小鼠脾脏分离出纯CD4^+、CDS8^+T细胞,在体外分别经多... 详细信息

目的探讨多房棘球蚴感染宿主CD4^+T淋巴细胞缺失的机制,多房棘球蚴感染与宿主T淋巴细胞亚群凋亡相互关系。方法利用尼龙柱和补体法从多房棘球蚴感染12wk,25wk和正常对照组Ba.B/c 小鼠脾脏分离出纯CD4^+、CDS8^+T细胞,在体外分别经多房棘球蚴抗原(Ema.)、抗CD3抗原(a.ti-CD3)、白细胞介素-2(IL-2)、肿瘤坏死因子α(TNFα)及商陆丝裂原(PWM)刺激培养16h,用DNa.胶电泳分析;透射电镜观察形态学变化;原位末端标记法(Tunel)和碘化丙锭(PI)双染后,流式细胞仪(FCM)分析凋亡细胞数和凋亡发生的细胞周期。结果感染25wk小鼠,CD4^+T细胞DNa.泳图均出现典型“梯形”条带,透射电镜见染色质浓染、胞质出泡、凋亡小体形成;CD8^+T细胞DNa.泳国无梯形带,透射电镜见染色质浓染,电子密度增强。FCM分析,感染12wk小鼠,CD4^+、CD8^+T细胞凋亡数与正常对照组差异无显著性意义(P>0.05);感染25wk小鼠,CD4^+T细胞凋亡数较正常对照组显著增高(Pa.CD),使宿主处于免疫抑制状态。

关键词：多房棘球蚴感染宿主 CD4^+T淋巴细胞缺失机制白细胞介素-2 肿瘤坏死因子α

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