Mapping of QTL Associated with Seed Amino Acids Content in “MD96-5722” by “Spencer” RIL Population of Soybean Using SNP Markers
Mapping of QTL Associated with Seed Amino Acids Content in “MD96-5722” by “Spencer” RIL Population of Soybean Using SNP Markers作者机构：Plant Genomics and Biotechnology Laboratory Department of Biological Sciences Fayetteville State University Fayetteville NC USA Department of Plant Soil and Agricultural Systems Southern Illinois University Carbondale IL USA Crop Genetics Research Unit Agricultural Research Service USDA Stoneville MS USA The School of Arts and Sciences American University of Ras Al Khaimah Ras Al Khiamah United Arab Emirates
出 版 物：《Food and Nutrition Sciences》 (食品与营养科学（英文）)
年 卷 期：2015年第6卷第11期
页 面：974-984页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Soybean Amino Acids SNP QTLs Seed Composition Constituents
摘 要：Limited information is available on the genetic analysis of amino acid composition in soybean seeds. Previously, quantitative trait loci (QTLs) for seed isoflavones, protein, oil, and fatty acids were identified in the “MD96-5722 by “Spencer and other RIL populations. There were wide variations for these seed constituents among the RIL populations. Therefore, the objective of this study was to identify QTLs controlling different amino acids content in soybean seeds. To achieve this objective, ninety-two F5:7 recombinant inbred lines (RIL), developed from a cross of MD96-5722 and Spencer, using a total 5376 Single Nucleotide Polymorphism (SNPs) markers, were used. The RILs were genotyped by using 537 polymorphic, reliably segregating SNP markers, developed from the Illumina Infinium SoySNP6K BeadChip array. A total of 13 QTLs were identified with three QTLs for threonine on the linkage group (LG) A1, C2, and B2. Two QTLs were identified for each of the amino acids proline on LG D1a and B2, serine on LG A1 and C2, tryptophan on LG K and G, and cysteine on LG A1 and K. One QTL was identified for arginine on LG N and histidine on LG J. The new QTLs findings for seed amino acid will facilitate the development of soybean cultivars with higher protein and amino acid quality to help meet the industry and consumer needs.
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