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Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution

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Plant Communications 2024年第2期5卷 46-66页

作者： Pengtao Li Qiankun Liu Yangyang Wei Chaozhu Xing zhongping xu Fang Ding Yuling Liu Quanwei Lu Nan Hu Tao Wang Xiangqian Zhu Shuang Cheng Zhaoguo Li Zilin Zhao Yanfang Li Jiangping Han Xiaoyan Cai Zhongli Zhou Kunbo Wang Baohong Zhang Fang Liu Shuangxia Jin Renhai Peng College of Biology and Food Engineering Anyang Institute of TechnologyAnyangHenan 455000China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China Research Base Anyang Institute of TechnologyState Key Laboratory of Cotton BiologyAnyang 455000HenanChina School of Life Sciences Zhengzhou UniversityZhengzhouHenan 450001China Hubei Hongshan Laboratory National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanHubeiChina Hubei Key Laboratory of Plant Pathology Huazhong Agricultural UniversityWuhanHubei 430070China Department of Biology East Carolina UniversityGreenvilleNC 27858USA

Increasing soil salinization has led to severe reductions in plant yield and quality,and investigating the mo-lecular mechanism of salt stress response is therefore an urgent *** this study,we systematically analyzed the response of cotton roots to salt stress using single-cell transcriptomics technology;56281 high-quality cells were obtained from 5-day-old lateral root tips of Gossypium arboreum under natural growth conditions and different salt *** cell types with an array of novel marker genes were identified and confirmed by in situ RNA hybridization,and pseudotime analysis of some specific cell types revealed their potential differentiation *** changes in cell numbers under salt stress were observed for outer epidermal and inner endodermal cells,which were significantly enriched in response to stress,amide biosynthetic process,glutathione metabolism,and glycolysis/*** of differentially expressed genes identified in multiple comparisons revealed other functional ag-gregations concentrated on plant-type primary cell wall biogenesis,defense response,phenylpropanoid biosynthesis,and metabolic *** candidate differentially expressed genes encoding transcrip-tion factors or associated with plant hormones also responsive to salt stress were identified,and the func-tion of Ga03G2153,annotated as auxin-responsive GH3.6,was confirmed by virus-induced gene *** GaGH3.6-silenced plants showed a severe stress-susceptible phenotype,and physiological and biochemical measurements indicated that they suffered more significant oxidative *** results suggest that GaGH3.6 might participate in cotton salt tolerance by regulating redox *** thus construct a transcriptional atlas of salt-stressed cotton roots at single-cell resolution,enabling us to explore cellular heterogeneity and differentiation trajectories and providing valuable insights into the mo-lecular mechanisms that underlie plan

关键词： cotton salt stress scRNA-seq root cell types virus-induced gene silencing

基于网格搜索优化支持向量机多分类参数识别不同工艺酱酒的应用研究

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中国酿造 2024年第6期43卷 213-217页

作者：陈旭东许忠平童凯王德良四川轻化工大学生物工程学院四川宜宾644005 中国食品发酵工业研究院有限公司北京100015 国家酒类品质与安全国际联合研究中心北京100015 天津科技大学生物工程学院天津300457

为提升支持向量机(SVM)在不同工艺酱酒分类预测中的准确度,该实验利用网格搜索优化支持向量机参数,建立最优参数的支持向量机分类预测模型。通过对不同工艺酱香型白酒客观结构特征定量分析,将提取的特征信息数据经过预处理(异常值处理... 详细信息

为提升支持向量机(SVM)在不同工艺酱酒分类预测中的准确度,该实验利用网格搜索优化支持向量机参数,建立最优参数的支持向量机分类预测模型。通过对不同工艺酱香型白酒客观结构特征定量分析,将提取的特征信息数据经过预处理(异常值处理、归一化操作等)后存储为样本数据集。其中样本数据分为训练样本与测试样本,通过训练样本对最优参数的SVM白酒品牌分类预测模型进行训练,测试样本对模型进行预测分类。经过试验验证,该模型的不同工艺分类识别率达到94.44%,较传统的SVM等分类算法能够快速、有效地对不同工艺的酱酒进行分类识别,显著改善分类的精度,改进后的方法实现过程也比较简单。

关键词：不同工艺酱酒支持向量机网格搜索分类预测

计及不可调度DG的孤岛串级交流微电网功率控制方法

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电源学报 2024年第3期22卷 298-306页

作者：王一妹李俊领曾杰许中平郑州电力高等专科学校信息通信学院郑州450000 国网西藏电力有限公司拉萨850000

针对由可调度和不可调度分布式电源DG(distributed generation)组成的串级孤岛微电网MGs(microgrids)功率控制机理复杂、电压和频率调节难度大等问题,提出了一种新型分散主从功率分配控制方法,以实现含二类DG串级孤岛微电网的电压、频... 详细信息

针对由可调度和不可调度分布式电源DG(distributed generation)组成的串级孤岛微电网MGs(microgrids)功率控制机理复杂、电压和频率调节难度大等问题,提出了一种新型分散主从功率分配控制方法,以实现含二类DG串级孤岛微电网的电压、频率调节以及功率分配。该方法分别设计了主DG和从DG的控制方法,其中,主DG负责调节微电网的频率和电压,从属DG的MPPT可在一定母线电压下运行,其可最大限度地利用不可调度DG的输出功率,在轻负荷条件下可采用自动功率削减,并有效保障系统的安全稳定性。最后,通过仿真和实验验证了所提方法的有效性。

关键词：分散控制不可调度单元功率削减串级微电网

改进的Faster R-CNN算法及其在电力作业安全穿戴检测中的应用

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武汉大学学报（工学版） 2024年第4期57卷 462-469页

作者：李强赵峰李博强许中平余金沄秦亮国网信息通信产业集团有限公司北京102211 武汉大学电气与自动化学院湖北武汉430072 北京国网信通埃森哲信息技术有限公司北京100052

运维人员正确的安全穿戴是确保电力作业安全的重要措施,计算机视觉深度学习算法为电力作业安全穿戴监管提供了一种新的手段。Faster R-CNN(faster region-based convolutional neural network)是一种有效的目标检测方法,在其基础上进行... 详细信息

运维人员正确的安全穿戴是确保电力作业安全的重要措施,计算机视觉深度学习算法为电力作业安全穿戴监管提供了一种新的手段。Faster R-CNN(faster region-based convolutional neural network)是一种有效的目标检测方法,在其基础上进行改进,首先以轻量级卷积神经网络EfficientNetV2(efficient network version 2)作为Faster RCNN的骨干,均衡提升算法的检测精度与速度;然后,在RPN(region proposal network)前引入注意力机制CBAM(convolutional block attention module)进一步提升检测精度。结合实际电力作业安全穿戴检测场景对该方法进行测试实验,结果表明,相对目前以VGG-16(visual geometry group-16)、ResNet-50(residual network-50)为骨干的Faster R-CNN算法而言,改进Faster R-CNN算法的检测精度和速度均得到提高,其平均精度均值(mean average precision,mAP)达到85.46%,检测速度达到47.82帧/s(frames per second,FPS),一定程度上提升了Faster R-CNN在电力作业安全穿戴检测中的应用能力。

关键词：电力作业安全穿戴 Faster R-CNN EfficientNetV2 CBAM

体外膜肺氧合转流方式对肺移植受者术后生化指标的影响

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实用器官移植电子杂志 2024年第4期12卷 292-296页

作者：徐忠平王大鹏田静董妍许红阳南京医科大学附属无锡人民医院重症医学科江苏无锡214023

目的对比分析肺移植受者采用不同的体外膜肺氧合(extracorporeal membrane oxygenation,ECMO)转流方式的术后7 d内的血红蛋白、血小板以及肝肾功能情况。方法回顾性分析2021年4月至2022年12月于南京医科大学附属无锡人民医院就诊并接受... 详细信息

目的对比分析肺移植受者采用不同的体外膜肺氧合(extracorporeal membrane oxygenation,ECMO)转流方式的术后7 d内的血红蛋白、血小板以及肝肾功能情况。方法回顾性分析2021年4月至2022年12月于南京医科大学附属无锡人民医院就诊并接受肺移植的144例患者临床资料,根据肺移植患者是否接受ECMO以及转流方式不同分为3组:无ECMO组(n=19),V-V ECMO组(n=107)和V-A ECMO组(n=18)。并采用卡方或似然比检验进行比较。结果与无ECMO治疗组相比,V-V ECMO和V-A ECMO组患者术后首次的血红蛋白更低,V-V ECMO组术后第2天的血小板更低,V-A ECMO组2~4 d的血小板水平更低。V-V ECMO和V-A ECMO组术后7 d内的血红蛋白和血小板比较差异并无统计学意义(P>0.05)。与无ECMO组比较,在术后第2天,V-V ECMO组患者肌酐较低,在术后3 d内,V-A ECMO组肌酐较无ECMO组低,在术后4~7 d组间无显著差异。术后3~4 d,V-A ECMO组受者肌酐较V-V ECMO组低。对于肾小球滤过率,无ECMO组与V-V ECMO组患者无显著差异,无ECMO组受者在术后4 d内的肾小球滤过率较V-A ECMO组低,在V-V和V-A两组中,V-V ECMO组在术后7 d内的肾小球滤过率较低,差异有统计学意义(P<0.05)。此外,V-A ECMO组患者术后第3天的胆红素水平比无ECMO组更高。结论本研究发现,ECMO治疗对于患者术后的血小板水平以及肾小球滤过率有一定的影响,且V-A转流方式下患者的术后预后指标要比V-V转流方式佳。

关键词：体外膜肺氧合肺移植肝肾功能

Improved edge lightweight YOLOv4 and its application in on-site power system work

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Global Energy Interconnection 2022年第2期5卷 168-180页

作者： Kexin Li Liang Qin Qiang Li Feng Zhao zhongping xu Kaipei Liu School of Electrical Engineering and Automation Wuhan UniverstiyWuhan430072P.R.China State Grid Information&Telecommunication Group Co. LtdBeijing102211P.R.China Beijing State Grid Information&Telecommunication Accenture Information Technology Co. Ltd.Beijing 100052P.R.China

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and *** to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively *** study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the ***,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature ***,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved ***,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on *** to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

关键词： On-site power system work YOLOv4-Tiny Convolution block attention mechanism Efficient channel attention Optimized training methods.

Transcriptome and metabolome analysis reveal that oral secretions from Helicoverpa armigera and Spodoptera litura influence wound-induced host response in cotton

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The Crop Journal 2020年第6期8卷 929-942页

作者： Huan Si Hongling Liu Yiwen Sun zhongping xu Sijia Liang Bo Li Xiao Ding Jianying Li Qiongqiong Wang Lin Sun Xianlong Zhang Shuangxia Jin National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Cotton(Gossypium hirsutum)is an important fiber crop *** attack causes cotton yield and quality ***,little is known about the mechanism of cotton response to insect *** simulated insect feeding by applying insect oral secretions(OS)to wounds,and combined transcriptome and metabolome analysis to investigate how OS from two major pest species(Helicoverpa armigera and Spodoptera litura)affect cotton defense *** found that respectively 12,668 and 13,379 genes were differentially expressed in comparison with wounding *** addition of OS,the jasmonic acid signaling pathway was rapidly and strongly induced,whereas genes involved in salicylic acid biosynthesis were *** constructing a coexpression gene network,we identified a hub gene encoding a leucine-rich repeat receptor kinase that may play an important role in early signal recognition and *** from the two insect species altered the abundance of flavonoid-related compounds in different *** remained in lower concentration after OS application than after wounding alone,suggesting a suppressive effect of OS on cotton defense *** study illustrated transcriptional and metabolic changes of cotton in responding to OS from two chewing insect species,identified potential key response genes,and revealed evidence for OS inhibition of wounding-induced cotton defense response.

关键词： Oral secretions Helicoverpa armigera Spodoptera litura Cotton Host plant defense

不同地区酱香型白酒风格特征及风味物质构成差异相关性研究

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中国酿造 2024年第2期43卷 35-42页

作者：许忠平梁明锋张娇娇雷显仲沈世明张明松孙志伟刘海坡张翠英韩兴林天津科技大学生物工程学院工业发酵微生物教育部重点实验室天津300457 中国食品发酵工业研究院有限公司北京100015 国家酒类品质与安全国际联合研究中心北京100015 贵州迎宾酒股份有限公司贵州遵义564500 中国酒业协会北京100831

采用感官评价和气相色谱(GC)法探究不同地区酱香型白酒感官特征和骨架风味物质含量,并对结果进行主成分分析(PCA)及Spearman相关性分析,解析不同地区酱香型白酒感官特征与骨架风味物质的差异及相关性。结果表明,贵州地区酱香型白酒酱香... 详细信息

采用感官评价和气相色谱(GC)法探究不同地区酱香型白酒感官特征和骨架风味物质含量,并对结果进行主成分分析(PCA)及Spearman相关性分析,解析不同地区酱香型白酒感官特征与骨架风味物质的差异及相关性。结果表明,贵州地区酱香型白酒酱香、曲香和焦香得分较高,南方其他地区花果香和草木香较突出,山东地区和北方其他地区醇香和粮香风格明显。风味物质分析结果发现,贵州地区酱香型白酒酯类含量最高,山东地区和南方其他地区酱香型白酒醇类含量较高,北方其他地区酱香型白酒羰基类含量较低,且贵州地区的酱香型白酒乙酸乙酯和乳酸乙酯的比率均在1.4~1.9范围内。PCA结果表明,贵州地区酱香型白酒风味物质结构相似性最高。Spearman相关性分析结果表明,酱香、焦香、曲香和陈香被聚为一类,与正丁醇、3-羟基-2-丁酮、异戊酸乙酯、辛酸乙酯、异戊醛和糠醛呈极显著正相关(P<0.01)。

关键词：酱香型白酒不同地区风格特征骨架风味物质主成分分析相关性

Identification and selection of resistance to Bemisia tabaci among 550 cotton genotypes in the field and greenhouse experiments

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Frontiers of Agricultural Science and Engineering 2018年第2期5卷 236-252页

作者： Lizhen ZHU Jianying LI zhongping xu Hakim MANGHWAR Sijia LIANG Suli LI Muna ALARIQI Shuangxia JIN Xianlong ZHANG National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Plants have developed sophisticated systems to cope with herbivore challenge, including morphological barriers and secondary metabolites to reduce damage. In this study, 550 Gossypium genotypes were evaluated for whitefly(Bemisia tabaci) resistance in five experiments including two in the field and three in the greenhouse, with23 resistant and 19 susceptible genotypes ***-resistance index determination showed that a leaf having a high density of hairs had resistance to whitfly egg/nymph production. Longer leaf hairs were also important for resistance. This study revealed that okra shaped leaves reduced adult whitefly oviposition preference, while glabrous leaves and high hair density helped not only in the reduction of the adults but also decreased oviposition preference. Gossypol was also observed to be involved in the reduction of adult whitefly development and/or survival.

关键词： Bemisia tabaci Gossypium genotypes gossypol leaf hair density leaf hair length